PMID- 27840902
OWN - NLM
STAT- MEDLINE
DCOM- 20170417
LR  - 20211201
IS  - 1791-2423 (Electronic)
IS  - 1019-6439 (Linking)
VI  - 49
IP  - 6
DP  - 2016 Dec
TI  - Identification of a novel alternative splicing transcript variant of the 
      suppressor of fused: Relationship with lymph node metastasis in pancreatic ductal 
      adenocarcinoma.
PG  - 2611-2619
LID - 10.3892/ijo.2016.3753 [doi]
AB  - Pancreatic ductal adenocarcinoma (PDAC) is one of the most fatal diseases, and 
      the median survival time is very short. Upregulation of hedgehog signaling 
      pathway activity is a vital factor in pathogenesis of PDAC. However, as a 
      negative regulator of hedgehog signaling, it is not very clear what role the 
      suppressor of fused (SUFU) plays in PDAC tissue. In our study for the 
      identification of alternative splicing transcripts of SUFU gene in human PDAC 
      cells, a novel transcript variant of SUFU (SUFUvN) was discovered by 3' rapid 
      amplification of cDNA ends (3'RACE) and cDNA clone. SUFUvN contained an 
      additional new protein-coding exon compared with the transcript variant 1 of SUFU 
      (SUFUv1, NM_016169) published in NCBI website. The sequence of the new 
      protein-coding exon was the same as a fragment of intron between exon 10 and 11 
      of SUFUv1. Thus, an exon skipping occurred in transcription of SUFUv1. Compared 
      with the expression vector of SUFUvN transfected PDAC cells, the corresponding 
      protein expression encoded by SUFUvN (SUFU isoform N) was detected in PDAC 
      tissue. Furthermore, it was observed that elevated SUFUvN transcription level was 
      related with lymph node metastasis in PDAC tissues, while neither SUFUv1 nor 
      transcript variant 2 of SUFU (SUFUv2, NM_001178133) did. Our data indicate that 
      there exists a novel transcript variant of SUFU which can be transcribed and 
      translated into corresponding protein and its transcription is related with 
      metastasis of lymph nodes in PDAC.
FAU - Xu, Qing
AU  - Xu Q
AD  - Department of Gastroenterology in Changhai Hospital, Second Military Medical 
      University, Shanghai 200433, P.R. China.
FAU - Gao, Jun
AU  - Gao J
AD  - Department of Gastroenterology in Changhai Hospital, Second Military Medical 
      University, Shanghai 200433, P.R. China.
FAU - Li, Zhaoshen
AU  - Li Z
AD  - Department of Gastroenterology in Changhai Hospital, Second Military Medical 
      University, Shanghai 200433, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20161103
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (GLI2 protein, human)
RN  - 0 (GLI3 protein, human)
RN  - 0 (Hedgehog Proteins)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Repressor Proteins)
RN  - 0 (SUFU protein, human)
RN  - 0 (Zinc Finger Protein Gli2)
RN  - 0 (Zinc Finger Protein Gli3)
SB  - IM
MH  - Aged
MH  - Alternative Splicing/*genetics
MH  - Carcinoma, Pancreatic Ductal/genetics/*pathology
MH  - Cell Line, Tumor
MH  - Cloning, Molecular
MH  - Female
MH  - Hedgehog Proteins/metabolism
MH  - Humans
MH  - Kruppel-Like Transcription Factors/metabolism
MH  - Lymph Nodes/*pathology
MH  - Lymphatic Metastasis/genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Nerve Tissue Proteins/metabolism
MH  - Nuclear Proteins/metabolism
MH  - Pancreatic Ducts/pathology
MH  - Pancreatic Neoplasms/genetics/*pathology
MH  - Polymerase Chain Reaction
MH  - Protein Isoforms/genetics/metabolism
MH  - Repressor Proteins/*genetics
MH  - Zinc Finger Protein Gli2
MH  - Zinc Finger Protein Gli3
EDAT- 2016/11/15 06:00
MHDA- 2017/04/18 06:00
CRDT- 2016/11/15 06:00
PHST- 2016/08/08 00:00 [received]
PHST- 2016/10/19 00:00 [accepted]
PHST- 2016/11/15 06:00 [pubmed]
PHST- 2017/04/18 06:00 [medline]
PHST- 2016/11/15 06:00 [entrez]
AID - 10.3892/ijo.2016.3753 [doi]
PST - ppublish
SO  - Int J Oncol. 2016 Dec;49(6):2611-2619. doi: 10.3892/ijo.2016.3753. Epub 2016 Nov 
      3.