PMID- 27840933
OWN - NLM
STAT- MEDLINE
DCOM- 20170407
LR  - 20170407
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 14
IP  - 6
DP  - 2016 Dec
TI  - Nobiletin ameliorates isoflurane-induced cognitive impairment via antioxidant, 
      anti-inflammatory and anti-apoptotic effects in aging rats.
PG  - 5408-5414
LID - 10.3892/mmr.2016.5919 [doi]
AB  - A recent study reported that nobiletin is an active ingredient in Fructus 
      Aurantii immaturus and Pericarpium Citri Reticulatae, which may be capable of 
      preventing ischemic stroke. Therefore, the present study aimed to determine the 
      neuroprotective effects of nobiletin, and to evaluate whether it could ameliorate 
      isoflurane‑induced cognitive impairment via antioxidant, anti‑inflammatory and 
      anti‑apoptotic effects in aging rats. Male Sprague‑Dawley rats (age, 18 months) 
      were used to analyze the neuroprotective effects of nobiletin. Morris water maze 
      test was used to determine cognitive competence. Enzyme‑linked immunosorbent 
      assay and western blot analysis were also used to quantify nuclear factor‑kappaB, 
      tumor necrosis factor (TNF)‑alpha, IL‑1beta, IL‑6, glutathione, (GSH), GSH‑peroxidase, 
      superoxide dismutase and malondialdehyde concentration and relevant protein 
      expression levels Cognitive competence was increased in isoflurane-treated rats 
      following treatment with nobiletin. In addition, as expected, nobiletin exerted 
      antioxidant, anti-inflammatory and anti‑apoptotic effects on isoflurane‑induced 
      cognitive impairment in aging rats. Treatment with nobiletin induced the 
      activation of phosphorylated (p)‑Akt, p‑cAMP response element binding protein 
      (CREB) and brain‑derived neurotrophic factor (BDNF) protein expression and 
      reduced the levels of B‑cell lymphoma 2‑associated X protein (Bax) in 
      isoflurane‑induced rats. In conclusion, the present study demonstrated that 
      nobiletin may ameliorate isoflurane-induced cognitive impairment through 
      antioxidant, anti‑inflammatory and anti‑apoptotic effects via modulation of Akt, 
      Bax, p‑CREB and BDNF in aging rats. These findings provide support for the 
      molecular mechanisms underlying the effects of nobiletin treatment on 
      isoflurane-induced damage.
FAU - Bi, Junying
AU  - Bi J
AD  - Department of Anesthesiology, Qilu Hospital, Shandong University, Jinan, Shandong 
      250012, P.R. China.
FAU - Zhang, Haiyan
AU  - Zhang H
AD  - Gynaecology Ward‑1, Linyi People's Hospital, Linyi, Shandong 276000, P.R. China.
FAU - Lu, Jing
AU  - Lu J
AD  - Department of Anesthesiology, Linyi People's Hospital, Linyi, Shandong 276000, 
      P.R. China.
FAU - Lei, Weifu
AU  - Lei W
AD  - Department of Anesthesiology, Qilu Hospital, Shandong University, Jinan, Shandong 
      250012, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20161101
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Cytokines)
RN  - 0 (Flavones)
RN  - 0 (Inflammation Mediators)
RN  - 0 (bcl-2-Associated X Protein)
RN  - CYS9AKD70P (Isoflurane)
RN  - D65ILJ7WLY (nobiletin)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Aging/*metabolism/psychology
MH  - Animals
MH  - Anti-Inflammatory Agents/chemistry/*pharmacology
MH  - Antioxidants/chemistry/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cognitive Dysfunction/*chemically induced/drug therapy/*metabolism
MH  - Cyclic AMP Response Element-Binding Protein/metabolism
MH  - Cytokines/metabolism
MH  - Flavones/chemistry/*pharmacology
MH  - Inflammation Mediators
MH  - Isoflurane/*adverse effects
MH  - Male
MH  - Maze Learning/drug effects
MH  - Memory/drug effects
MH  - Oxidation-Reduction/drug effects
MH  - Oxidative Stress/drug effects
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Rats
MH  - bcl-2-Associated X Protein/metabolism
EDAT- 2016/11/15 06:00
MHDA- 2017/04/08 06:00
CRDT- 2016/11/15 06:00
PHST- 2015/08/07 00:00 [received]
PHST- 2016/08/22 00:00 [accepted]
PHST- 2016/11/15 06:00 [pubmed]
PHST- 2017/04/08 06:00 [medline]
PHST- 2016/11/15 06:00 [entrez]
AID - 10.3892/mmr.2016.5919 [doi]
PST - ppublish
SO  - Mol Med Rep. 2016 Dec;14(6):5408-5414. doi: 10.3892/mmr.2016.5919. Epub 2016 Nov 
      1.