PMID- 27841196
OWN - NLM
STAT- MEDLINE
DCOM- 20181105
LR  - 20181105
IS  - 0028-3886 (Print)
IS  - 0028-3886 (Linking)
VI  - 64
IP  - 6
DP  - 2016 Nov-Dec
TI  - Fluorescence in situ hybridization for chromosome 14q deletion in subsets of 
      meningioma segregated by MIB-1 labelling index.
PG  - 1256-1263
LID - 10.4103/0028-3886.193768 [doi]
AB  - AIM: To correlate histopathological grading of meningiomas segregated into 
      subgroups based on the MIB-1 labelling index (MIB-1 LI) with chromosomal loss of 
      14q using fluorescence in situ hybridization (FISH). SETTINGS AND DESIGN: 
      Retrospective study conducted in a tertiary hospital. METHODS AND MATERIAL: 
      Forty-six cases from January to December 2011 were segregated into 5 categories 
      based on the MIB-1 LI. Slides were reviewed to ascertain the grade. 
      Immunohistochemical staining for MIB-1 was performed using a Ventana Benchmark XT 
      autostainer. Commercially available FISH paraffin pretreatment kit and 
      SpectrumOrange fluorophore labelled probe were used. The Statistical Package for 
      the Social Sciences version 16.0 for Windows was used for statistical analysis. 
      RESULTS: There were 21 World Health Organisation (WHO) grade I, 24 grade II, and 
      1 grade III meningiomas. There was a statistically significant difference between 
      the mean duration of symptoms, maximum dimension, and the MIB-1 LI of grade I and 
      grade II meningiomas. 33.3% grade I cases showed 14q deletion, compared to 84% of 
      grade II and III meningiomas. Histologically, hypercellularity, small cell 
      formation, prominent nucleoli, and sheet-like growth were significantly 
      associated with 14q deletion. All brain invasive meningiomas had 14q deletion. As 
      MIB-1% increased, the prevalence of deletions was significantly higher. The mean 
      MIB-1 of the 7 grade I meningiomas that had 14q deletions was 8.86 +/- 1.95% when 
      compared to 4.14 +/- 1.35% for those without 14q deletions. CONCLUSIONS: A strong 
      association existed between histologic grade, MIB-1 LI, and the presence of 
      chromosome 14q deletion. Association of high MIB-1 LI with 14q deletions, even in 
      meningiomas with a Grade I histology, defines a distinct subset of benign 
      meningiomas.
FAU - Gupta, Noopur
AU  - Gupta N
AD  - Department of General Pathology, Christian Medical College, Vellore, Tamil Nadu, 
      India.
FAU - Chacko, Geeta
AU  - Chacko G
AD  - Department of General Pathology, Christian Medical College, Vellore, Tamil Nadu, 
      India.
FAU - Chacko, Ari G
AU  - Chacko AG
AD  - Department of Neurological Sciences, Christian Medical College, Vellore, Tamil 
      Nadu, India.
FAU - Rajshekhar, Vedantam
AU  - Rajshekhar V
AD  - Department of Neurological Sciences, Christian Medical College, Vellore, Tamil 
      Nadu, India.
FAU - Jayprakash, Muyilil
AU  - Jayprakash M
AD  - Division of Gastrointestinal Sciences, Christian Medical College, Vellore, Tamil 
      Nadu, India.
LA  - eng
PT  - Journal Article
PL  - India
TA  - Neurol India
JT  - Neurology India
JID - 0042005
SB  - IM
MH  - *Chromosome Deletion
MH  - *Chromosomes, Human, Pair 14
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Meningeal Neoplasms/*genetics
MH  - Meningioma/*genetics
MH  - Retrospective Studies
EDAT- 2016/11/15 06:00
MHDA- 2018/11/06 06:00
CRDT- 2016/11/15 06:00
PHST- 2016/11/15 06:00 [entrez]
PHST- 2016/11/15 06:00 [pubmed]
PHST- 2018/11/06 06:00 [medline]
AID - ni_2016_64_6_1256_193768 [pii]
AID - 10.4103/0028-3886.193768 [doi]
PST - ppublish
SO  - Neurol India. 2016 Nov-Dec;64(6):1256-1263. doi: 10.4103/0028-3886.193768.