PMID- 27842852 OWN - NLM STAT- MEDLINE DCOM- 20180615 LR - 20181202 IS - 1879-1344 (Electronic) IS - 0144-8617 (Linking) VI - 156 DP - 2017 Jan 20 TI - Characteristics of glucomannan isolated from fresh tuber of Porang (Amorphophallus muelleri Blume). PG - 56-63 LID - S0144-8617(16)31032-3 [pii] LID - 10.1016/j.carbpol.2016.08.080 [doi] AB - Porang is a potential source of glucomannan. This research objective was to find a direct glucomannan isolation method from fresh porang corm to produce high purity glucomannan. Two isolation methods were performed. In first method, sample was water dissolved using Al(2)(SO(4))(3) as flocculant for 15 (AA15) or 30 (AA30) minutes with purification. In second method, sample was repeatedly milled using ethanol as solvent and filtered for 5 (EtOH5) or 7 (EtOH7) times without purification. The characteristics of obtained glucomannan were compared to those of commercial porang flour (CPF) and purified konjac glucomannan (PKG). High purity (90.98%), viscosity (27,940 cps) and transparency (57.74%) of amorphous glucomannan were isolated by EtOH7. Ash and protein level significantly reduced to 0.57% and 0.31%, respectively, with no starch content. Water holding capacity (WHC) of EtOH7 glucomannan significantly enhanced, whereas its solubility was lower than those of PKG due to its ungrounded native granular form. CI - Copyright (c) 2016 Elsevier Ltd. All rights reserved. FAU - Yanuriati, Anny AU - Yanuriati A AD - Department of Agricultural Technology, Sriwijaya University, Jl. Palembang Prabumulih Km. 32, Inderalaya, Ogan Ilir 30662, South Sumatra, Indonesia; Faculty of Agricultural Technology, Gadjah Mada University, Jl. Flora No.1, Yogyakarta 55281, Indonesia. Electronic address: annyyanuriati@fp.unsri.ac.id. FAU - Marseno, Djagal Wiseso AU - Marseno DW AD - Faculty of Agricultural Technology, Gadjah Mada University, Jl. Flora No.1, Yogyakarta 55281, Indonesia. FAU - Rochmadi AU - Rochmadi AD - Chemical Engineering Department, Faculty of Engineering, Gadjah Mada University, Jl. Grafika No. 2, Yogyakarta 55281, Indonesia. FAU - Harmayani, Eni AU - Harmayani E AD - Faculty of Agricultural Technology, Gadjah Mada University, Jl. Flora No.1, Yogyakarta 55281, Indonesia. LA - eng PT - Journal Article DEP - 20160826 PL - England TA - Carbohydr Polym JT - Carbohydrate polymers JID - 8307156 RN - 0 (Mannans) RN - 36W3E5TAMG ((1-6)-alpha-glucomannan) SB - IM MH - Amorphophallus/*chemistry MH - Flour MH - Mannans/*analysis MH - Plant Tubers/*chemistry MH - Viscosity OTO - NOTNLM OT - Amorphophallus muelleri Blume OT - Characteristics OT - Fresh tuber OT - Glucomannan OT - Isolation OT - Porang EDAT- 2016/11/16 06:00 MHDA- 2018/06/16 06:00 CRDT- 2016/11/16 06:00 PHST- 2016/03/24 00:00 [received] PHST- 2016/08/15 00:00 [revised] PHST- 2016/08/25 00:00 [accepted] PHST- 2016/11/16 06:00 [entrez] PHST- 2016/11/16 06:00 [pubmed] PHST- 2018/06/16 06:00 [medline] AID - S0144-8617(16)31032-3 [pii] AID - 10.1016/j.carbpol.2016.08.080 [doi] PST - ppublish SO - Carbohydr Polym. 2017 Jan 20;156:56-63. doi: 10.1016/j.carbpol.2016.08.080. Epub 2016 Aug 26.