PMID- 27842980
OWN - NLM
STAT- MEDLINE
DCOM- 20171204
LR  - 20190816
IS  - 1477-2566 (Electronic)
IS  - 1465-3249 (Linking)
VI  - 19
IP  - 1
DP  - 2017 Jan
TI  - Establishment, characterization and long-term culture of human endocrine 
      pancreas-derived microvascular endothelial cells.
PG  - 141-152
LID - S1465-3249(16)30565-5 [pii]
LID - 10.1016/j.jcyt.2016.10.005 [doi]
AB  - BACKGROUND: In vitro primary cultures of microvascular endothelial cells from 
      endocrine pancreas are difficult to obtain, but can be a very helpful tool for 
      studies of islet biology, transplantation and regenerative medicine. METHODS: We 
      applied a protocol recently described for the isolation and culture of brain 
      microvascular endothelial cells (EC) on human pancreatic islets. EC obtained were 
      characterized in terms of morphological (light and transmission electron 
      microscopy), phenotypical (by immunofluorescence and flow cytometry) and 
      functional (cord formation assay and protein secretion by multiplex bead-based 
      assay) characteristics. RESULTS: EC were obtained from 25% of islet preparations 
      processed. Two primary endothelial cell lines showed high proliferative potential 
      and were deeply characterized: they presented endothelial cell morphology and 
      expressed CD31, CD49a, CD49e, CD34, von Willebrand Factor (vWF), Vascular 
      Endothelial CAdherin (VE-CAD), Tyrosine Kinase with Ig and EGF Homology Domains-2 
      (TIE2), Vascular Endothelial Growth Factor Receptor 1 (VEGFR1), Ulex lectin and 
      the endothelium endocrine-specific marker nephrin. Besides, they were able to 
      form cordons in vitro and secreted factors involved in the process of 
      angiogenesis such as Vascular Endothelial Growth Factor (VEGF), Monocyte 
      Chemotactic Protein 1 (MCP-1), interleukin (IL)-8 and Melanoma Growth Stimulatory 
      Activity Alpha (GROalpha). These cell lines were termed Human Islet Microvascular 
      Endothelial Cells (HIMEC). DISCUSSION: This study establishes a simple and 
      effective strategy for isolation and long-term culture of EC derived from human 
      pancreatic islet. HIMEC in culture preserve phenotype and functional properties 
      and are, therefore, a useful tool for future experiments of in vitro pancreas 
      modelling, co-transplantation with pancreatic islets, re-vascularization of 
      scaffold or matrix for regenerative medicine purposes.
CI  - Copyright (c) 2017 International Society for Cellular Therapy. Published by 
      Elsevier Inc. All rights reserved.
FAU - Sordi, Valeria
AU  - Sordi V
AD  - Diabetes Research Institute, Istituto di Ricovero e Cura a Carattere Scientifico 
      San Raffaele Scientific Institute, Milan, Italy. Electronic address: 
      sordi.valeria@hsr.it.
FAU - Ferri, Anna
AU  - Ferri A
AD  - Cellular Neurobiology Laboratory, Department of Cerebrovascular Diseases, IRCCS 
      Neurological Institute C. Besta, Milan, Italy.
FAU - Ceserani, Valentina
AU  - Ceserani V
AD  - Cellular Neurobiology Laboratory, Department of Cerebrovascular Diseases, IRCCS 
      Neurological Institute C. Besta, Milan, Italy.
FAU - Ciusani, Emilio
AU  - Ciusani E
AD  - Cellular Neurobiology Laboratory, Department of Cerebrovascular Diseases, IRCCS 
      Neurological Institute C. Besta, Milan, Italy.
FAU - Dugnani, Erica
AU  - Dugnani E
AD  - Diabetes Research Institute, Istituto di Ricovero e Cura a Carattere Scientifico 
      San Raffaele Scientific Institute, Milan, Italy.
FAU - Pellegrini, Silvia
AU  - Pellegrini S
AD  - Diabetes Research Institute, Istituto di Ricovero e Cura a Carattere Scientifico 
      San Raffaele Scientific Institute, Milan, Italy.
FAU - Nano, Rita
AU  - Nano R
AD  - Diabetes Research Institute, Istituto di Ricovero e Cura a Carattere Scientifico 
      San Raffaele Scientific Institute, Milan, Italy.
FAU - Pecciarini, Lorenza
AU  - Pecciarini L
AD  - Pathology Department, Istituto di Ricovero e Cura a Carattere Scientifico San 
      Raffaele Scientific Institute, Milan, Italy.
FAU - Pessina, Augusto
AU  - Pessina A
AD  - Department of Biomedical, Surgical and Dental Sciences, University of Milan, 
      Milan, Italy.
FAU - Pascucci, Luisa
AU  - Pascucci L
AD  - Department of Veterinary Medicine, University of Perugia, Perugia, Italy.
FAU - Piemonti, Lorenzo
AU  - Piemonti L
AD  - Diabetes Research Institute, Istituto di Ricovero e Cura a Carattere Scientifico 
      San Raffaele Scientific Institute, Milan, Italy.
FAU - Alessandri, Giulio
AU  - Alessandri G
AD  - Cellular Neurobiology Laboratory, Department of Cerebrovascular Diseases, IRCCS 
      Neurological Institute C. Besta, Milan, Italy.
LA  - eng
PT  - Journal Article
DEP - 20161111
PL  - England
TA  - Cytotherapy
JT  - Cytotherapy
JID - 100895309
RN  - 0 (Antigens, CD)
RN  - 0 (CXCL8 protein, human)
RN  - 0 (Cadherins)
RN  - 0 (Interleukin-8)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (cadherin 5)
RN  - 0 (von Willebrand Factor)
RN  - EC 2.7.10.1 (FLT1 protein, human)
RN  - EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-1)
SB  - IM
MH  - Antigens, CD/metabolism
MH  - Cadherins/metabolism
MH  - Cells, Cultured
MH  - Endothelial Cells/cytology/*metabolism
MH  - Endothelium, Vascular/*cytology
MH  - Humans
MH  - Interleukin-8/metabolism
MH  - Islets of Langerhans/*cytology
MH  - Microvessels/cytology
MH  - Vascular Endothelial Growth Factor A/metabolism
MH  - Vascular Endothelial Growth Factor Receptor-1
MH  - von Willebrand Factor/metabolism
OTO - NOTNLM
OT  - endothelial cell line
OT  - islets
OT  - pancreas
OT  - primary culture
EDAT- 2016/11/16 06:00
MHDA- 2017/12/05 06:00
CRDT- 2016/11/16 06:00
PHST- 2016/08/04 00:00 [received]
PHST- 2016/10/07 00:00 [revised]
PHST- 2016/10/12 00:00 [accepted]
PHST- 2016/11/16 06:00 [pubmed]
PHST- 2017/12/05 06:00 [medline]
PHST- 2016/11/16 06:00 [entrez]
AID - S1465-3249(16)30565-5 [pii]
AID - 10.1016/j.jcyt.2016.10.005 [doi]
PST - ppublish
SO  - Cytotherapy. 2017 Jan;19(1):141-152. doi: 10.1016/j.jcyt.2016.10.005. Epub 2016 
      Nov 11.