PMID- 27844056
OWN - NLM
STAT- MEDLINE
DCOM- 20171031
LR  - 20231229
IS  - 2373-2822 (Electronic)
IS  - 2373-2822 (Linking)
VI  - 3
IP  - 5
DP  - 2016 Sep-Oct
TI  - Hyperpolarization-Activated Currents and Subthreshold Resonance in Granule Cells 
      of the Olfactory Bulb.
LID - ENEURO.0197-16.2016
AB  - An important contribution to neural circuit oscillatory dynamics is the ongoing 
      activation and inactivation of hyperpolarization-activated currents (I(h)). 
      Network synchrony dynamics play an important role in the initial processing of 
      odor signals by the main olfactory bulb (MOB) and accessory olfactory bulb (AOB). 
      In the mouse olfactory bulb, we show that I(h) is present in granule cells (GCs), 
      the most prominent inhibitory neuron in the olfactory bulb, and that I(h) 
      underlies subthreshold resonance in GCs. In accord with the properties of I(h), 
      the currents exhibited sensitivity to changes in extracellular K(+) concentration 
      and ZD7288 (4-ethylphenylamino-1,2-dimethyl-6-methylaminopyrimidin chloride), a 
      blocker of I(h). ZD7288 also caused GCs to hyperpolarize and increase their input 
      resistance, suggesting that I(h) is active at rest in GCs. The inclusion of cAMP 
      in the intracellular solution shifted the activation of I(h) to less negative 
      potentials in the MOB, but not in the AOB, suggesting that channels with 
      different subunit composition mediate I(h) in these regions. Furthermore, we show 
      that mature GCs exhibit I(h)-dependent subthreshold resonance in the theta 
      frequency range (4-12 Hz). Another inhibitory subtype in the MOB, the 
      periglomerular cells, exhibited I(h)-dependent subthreshold resonance in the 
      delta range (1-4 Hz), while principal neurons, the mitral cells, do not exhibit 
      I(h)-dependent subthreshold resonance. Importantly, I(h) size, as well as the 
      strength and frequency of resonance in GCs, exhibited a postnatal developmental 
      progression, suggesting that this development of I(h) in GCs may differentially 
      contribute to their integration of sensory input and contribution to oscillatory 
      circuit dynamics.
FAU - Hu, Ruilong
AU  - Hu R
AD  - Department of Biology, University of Maryland , College Park, Maryland 20742.
FAU - Ferguson, Katie A
AU  - Ferguson KA
AD  - Neurobiology Course, Marine Biology Laboratory , Woods Hole, Massachusetts 02543.
FAU - Whiteus, Christina B
AU  - Whiteus CB
AUID- ORCID: 0000-0003-2093-7223
AD  - Neurobiology Course, Marine Biology Laboratory , Woods Hole, Massachusetts 02543.
FAU - Meijer, Dimphna H
AU  - Meijer DH
AD  - Neurobiology Course, Marine Biology Laboratory , Woods Hole, Massachusetts 02543.
FAU - Araneda, Ricardo C
AU  - Araneda RC
AUID- ORCID: 0000-0001-8728-3063
AD  - Department of Biology, University of Maryland , College Park, Maryland 20742.
LA  - eng
GR  - R01 AG049937/AG/NIA NIH HHS/United States
GR  - R01 DC009817/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20161104
PL  - United States
TA  - eNeuro
JT  - eNeuro
JID - 101647362
RN  - 0 (Cations, Monovalent)
RN  - 0 (Neurotransmitter Agents)
RN  - 0 (Pyrimidines)
RN  - 133059-99-1 (ICI D2788)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - E0399OZS9N (Cyclic AMP)
RN  - RWP5GA015D (Potassium)
SB  - IM
EIN - eNeuro. 2023 Dec 26;10(12):. PMID: 38148163
MH  - Animals
MH  - Cations, Monovalent/metabolism
MH  - Cyclic AMP/metabolism
MH  - Electroporation
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Membrane Potentials/drug effects/*physiology
MH  - Mice, Inbred C57BL
MH  - Neural Inhibition/drug effects/physiology
MH  - Neural Pathways/cytology/drug effects/growth & development/physiology
MH  - Neurons/cytology/drug effects/*physiology
MH  - Neurotransmitter Agents/pharmacology
MH  - Olfactory Bulb/cytology/drug effects/*growth & development/*physiology
MH  - Patch-Clamp Techniques
MH  - Potassium/metabolism
MH  - Pyrimidines/pharmacology
MH  - Theta Rhythm
MH  - Tissue Culture Techniques
PMC - PMC5095762
OTO - NOTNLM
OT  - HCN
OT  - granule cell
OT  - neurogenesis
OT  - olfactory bulb
OT  - resonance
EDAT- 2016/11/16 06:00
MHDA- 2017/11/01 06:00
PMCR- 2016/11/04
CRDT- 2016/11/16 06:00
PHST- 2016/07/09 00:00 [received]
PHST- 2016/10/19 00:00 [revised]
PHST- 2016/10/24 00:00 [accepted]
PHST- 2016/11/16 06:00 [entrez]
PHST- 2016/11/16 06:00 [pubmed]
PHST- 2017/11/01 06:00 [medline]
PHST- 2016/11/04 00:00 [pmc-release]
AID - eN-NWR-0197-16 [pii]
AID - 10.1523/ENEURO.0197-16.2016 [doi]
PST - epublish
SO  - eNeuro. 2016 Nov 4;3(5):ENEURO.0197-16.2016. doi: 10.1523/ENEURO.0197-16.2016. 
      eCollection 2016 Sep-Oct.