PMID- 27844077
OWN - NLM
STAT- MEDLINE
DCOM- 20171103
LR  - 20180313
IS  - 1756-591X (Electronic)
IS  - 1756-5901 (Linking)
VI  - 8
IP  - 12
DP  - 2016 Dec 7
TI  - Fe-oxy adducts of heme-Abeta and heme-hIAPP complexes: intermediates in ROS 
      generation.
PG  - 1266-1272
AB  - Iron (Fe) is the most abundant transition metal ion in the human body and its 
      role, in the form of heme, has been implicated in Alzheimer's disease (AD) and 
      type 2 diabetes mellitus (T2Dm). Heme binds both amyloid beta (Abeta) and human 
      islet amyloid polypeptide (hIAPP) to form heme-Abeta and heme-hIAPP complexes, 
      respectively, and form reactive oxygen species (ROS) like H(2)O(2), O(2) (-)etc., 
      which are known to cause oxidative damage. However the intermediates involved 
      during ROS formation have not yet been isolated. In this study the oxygen bound 
      intermediates of both heme-Abeta(1-16) and heme-hIAPP(1-19) have been isolated and 
      characterized using absorption, EPR and resonance Raman (rR) spectroscopy. Fe-O 
      stretches have been found at 575 cm(-1) and 577 cm(-1) for heme-Abeta(1-16) and 
      heme-hIAPP(1-19) respectively. The oxy intermediates are stable at low 
      temperatures. The isolation of the intermediates reveals a mechanistic pathway of 
      ROS generation through the two heme complexes.
FAU - Seal, Manas
AU  - Seal M
AD  - Department of Inorganic Chemistry, Indian Association for the Cultivation of 
      Science, Jadavpur, Kolkata 700032, India. icsgd@iacs.res.in.
FAU - Mukherjee, Soumya
AU  - Mukherjee S
AD  - Department of Inorganic Chemistry, Indian Association for the Cultivation of 
      Science, Jadavpur, Kolkata 700032, India. icsgd@iacs.res.in.
FAU - Dey, Somdatta Ghosh
AU  - Dey SG
AD  - Department of Inorganic Chemistry, Indian Association for the Cultivation of 
      Science, Jadavpur, Kolkata 700032, India. icsgd@iacs.res.in.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Metallomics
JT  - Metallomics : integrated biometal science
JID - 101478346
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Iron Compounds)
RN  - 0 (Islet Amyloid Polypeptide)
RN  - 0 (Reactive Oxygen Species)
RN  - 42VZT0U6YR (Heme)
SB  - IM
MH  - Amyloid beta-Peptides/*metabolism
MH  - Heme/*metabolism
MH  - Humans
MH  - Iron Compounds/*metabolism
MH  - Islet Amyloid Polypeptide/*metabolism
MH  - Reactive Oxygen Species/*metabolism
EDAT- 2016/11/16 06:00
MHDA- 2017/11/04 06:00
CRDT- 2016/11/16 06:00
PHST- 2016/11/16 06:00 [pubmed]
PHST- 2017/11/04 06:00 [medline]
PHST- 2016/11/16 06:00 [entrez]
AID - 10.1039/c6mt00214e [doi]
PST - ppublish
SO  - Metallomics. 2016 Dec 7;8(12):1266-1272. doi: 10.1039/c6mt00214e.