PMID- 27845861
OWN - NLM
STAT- MEDLINE
DCOM- 20170526
LR  - 20171116
IS  - 1557-7600 (Electronic)
IS  - 1096-620X (Linking)
VI  - 19
IP  - 11
DP  - 2016 Nov
TI  - Apigetrin from Scutellaria baicalensis Georgi Inhibits Neuroinflammation in BV-2 
      Microglia and Exerts Neuroprotective Effect in HT22 Hippocampal Cells.
PG  - 1032-1040
AB  - Apigetrin is a flavonoid isolated from various herbal medicines such as 
      Scutellaria baicalensis Georgi, Matricaria chamomilla, Stachys tibetica Vatke, 
      and Teucrium gnaphalodes. In the present study, we investigated the inhibitory 
      effects of apigetrin on neuroinflammation using the BV-2 microglia cell line. Our 
      data revealed that apigetrin significantly reduced secretion and mRNA expression 
      of inflammatory cytokines, tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 
      (IL-6), in lipopolysaccharide (LPS)-stimulated BV-2 mouse microglia. Apigetrin 
      also significantly decreased LPS-mediated production of prostaglandin E(2) 
      (PGE(2)) level and nitric oxide (NO) production as well as expression of 
      cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) in BV-2 
      cells. In addition, apigetrin suppressed nuclear expression of nuclear factor 
      kappa B (NF-kappaB) in LPS-stimulated BV-2 cells. Furthermore, apigetrin 
      significantly impaired reactive oxygen species (ROS) generation and enhanced 
      expression of antioxidant enzymes, hempxygenase 1 (HO-1) and nuclear factor-like 
      2 (Nrf2), in BV-2 cells. Apigetrin also increased 
      2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging 
      activity, indicating antioxidative activity of apigetrin. Moreover, we found that 
      apigetrin inhibited hydrogen peroxide (H(2)O(2))-induced cell death in HT22 
      hippocampal cells. Overall, our findings indicate that apigetrin has inhibitory 
      effects on neuroinflammation as well as antioxidation and neuroprotection, 
      suggesting the potential prophylactic activity for neurodegenerative diseases 
      through the inter-regulation of neuroinflammation, oxidative stress, and neuronal 
      injury.
FAU - Lim, Hye-Sun
AU  - Lim HS
AD  - 1 Herbal Medicine Research Division, Korea Institute of Oriental Medicine , 
      Daejeon, Korea.
FAU - Kim, Ohn-Soon
AU  - Kim OS
AD  - 1 Herbal Medicine Research Division, Korea Institute of Oriental Medicine , 
      Daejeon, Korea.
FAU - Kim, Bu-Yeo
AU  - Kim BY
AD  - 1 Herbal Medicine Research Division, Korea Institute of Oriental Medicine , 
      Daejeon, Korea.
FAU - Jeong, Soo-Jin
AU  - Jeong SJ
AD  - 1 Herbal Medicine Research Division, Korea Institute of Oriental Medicine , 
      Daejeon, Korea.
AD  - 2 Korean Medicine Life Science, University of Science and Technology , Daejeon, 
      Korea.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Med Food
JT  - Journal of medicinal food
JID - 9812512
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Plant Extracts)
RN  - 0 (Scutellaria baicalensis extract)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 7OF2S66PCH (apigetrin)
RN  - 7V515PI7F6 (Apigenin)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Apigenin/isolation & purification/*pharmacology/toxicity
MH  - Cell Line
MH  - Cyclooxygenase 2/metabolism
MH  - Cytokines/metabolism
MH  - Hippocampus/*drug effects/immunology/metabolism/pathology
MH  - Inflammation/drug therapy/immunology/metabolism/pathology
MH  - Lipopolysaccharides/pharmacology
MH  - Mice
MH  - Microglia/*drug effects/immunology/metabolism/pathology
MH  - Neuroprotective Agents/chemistry/isolation & purification/*pharmacology
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase Type II/metabolism
MH  - Plant Extracts/chemistry/*pharmacology
MH  - Scutellaria baicalensis/*chemistry
MH  - Signal Transduction/drug effects
OTO - NOTNLM
OT  - Scutellaria baicalensis
OT  - anti-neuroinflammation
OT  - apigetrin
OT  - neuroprotection
EDAT- 2016/11/16 06:00
MHDA- 2017/05/27 06:00
CRDT- 2016/11/16 06:00
PHST- 2016/11/16 06:00 [entrez]
PHST- 2016/11/16 06:00 [pubmed]
PHST- 2017/05/27 06:00 [medline]
AID - 10.1089/jmf.2016.0074 [doi]
PST - ppublish
SO  - J Med Food. 2016 Nov;19(11):1032-1040. doi: 10.1089/jmf.2016.0074.