PMID- 27855370
OWN - NLM
STAT- MEDLINE
DCOM- 20170213
LR  - 20210103
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 40
IP  - 1-2
DP  - 2016
TI  - CD47 is a Potential Target for the Treatment of Laryngeal Squamous Cell 
      Carcinoma.
PG  - 126-136
AB  - BACKGROUND/AIMS: This study aims to investigate the effect of CD47 on the 
      development of laryngeal squamous cell carcinoma (LSCC) and the therapeutic 
      potential of monoclonal antibody against CD47 and its ligand SIRPalpha in the 
      treatment of LSCC. METHODS: We firstly detected the expressions of CD47 mRNA and 
      protein in LSCC and para-carcinoma tissues, introduced the most efficient 
      CD47siRNA sequence into LSCC cells by lentiviral transfection and employed three 
      monoclonal antibodies to evaluate their anti-LSCC effects in vitro and in vivo. 
      RESULTS: We observed that the mRNA and protein expressions of CD47 in LSCC tissue 
      had significant increase in LSCC tissues compared with those in para-carcinoma 
      tissue (p < 0.05). After the treatments of three monoclonal antibodies, i.e. 
      anti-SIRPalpha, anti-CD47 BRIC126, anti-CD47 B6H12.2, in rats transfected with Hep-2 
      cell, it has been showed that the mRNA and protein expressions of CD47 in LSCC 
      tissue decreased, macrophage efficiency was promoted when anti-SIRPalpha and/or 
      CD47siRNA were used, the amounts, viabilities and expressions of CD47 protein of 
      tumor cell were significantly inhibited. Additionally, combined use of CD47siRNA 
      and anti-SIRPalpha seemed more efficient than solo use of CD47siRNA/anti-SIRPalpha. 
      CONCLUSION: The results suggested a critical role of CD47 in LSCC development and 
      the promising treatment of antiCD47/SIRPalpha and/or CD47siRNA in LSCC.
CI  - (c) 2016 The Author(s) Published by S. Karger AG, Basel.
FAU - Yang, ChunPing
AU  - Yang C
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated 
      Hospital of Nanchang University, Nanchang, China.
FAU - Gao, ShuFeng
AU  - Gao S
FAU - Zhang, HaiZhen
AU  - Zhang H
FAU - Xu, Lian
AU  - Xu L
FAU - Liu, JianGuo
AU  - Liu J
FAU - Wang, Meiqun
AU  - Wang M
FAU - Zhang, ShaoRong
AU  - Zhang S
LA  - eng
PT  - Journal Article
DEP - 20161118
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (CD47 Antigen)
RN  - 0 (CD47 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Animals
MH  - CD47 Antigen/genetics/*metabolism
MH  - Carcinoma, Squamous Cell/genetics/pathology/*therapy
MH  - Cell Line, Tumor
MH  - Flow Cytometry
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/pathology/*therapy
MH  - Lentivirus/metabolism
MH  - Macrophages/metabolism
MH  - *Molecular Targeted Therapy
MH  - Phagocytosis
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA, Small Interfering/metabolism
MH  - Rats
MH  - Transfection
EDAT- 2016/11/18 06:00
MHDA- 2017/02/14 06:00
CRDT- 2016/11/18 06:00
PHST- 2016/10/04 00:00 [accepted]
PHST- 2016/11/18 06:00 [pubmed]
PHST- 2017/02/14 06:00 [medline]
PHST- 2016/11/18 06:00 [entrez]
AID - 000452530 [pii]
AID - 10.1159/000452530 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2016;40(1-2):126-136. doi: 10.1159/000452530. Epub 2016 Nov 
      18.