PMID- 27855402
OWN - NLM
STAT- MEDLINE
DCOM- 20170206
LR  - 20211203
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 39
IP  - 6
DP  - 2016
TI  - Up-Regulation of the Excitatory Amino Acid Transporters EAAT1 and EAAT2 by 
      Mammalian Target of Rapamycin.
PG  - 2492-2500
AB  - BACKGROUND: The excitatory amino-acid transporters EAAT1 and EAAT2 clear 
      glutamate from the synaptic cleft and thus terminate neuronal excitation. The 
      carriers are subject to regulation by various kinases. The EAAT3 isoform is 
      regulated by mammalian target of rapamycin (mTOR). The present study thus 
      explored whether mTOR influences transport by EAAT1 and/or EAAT2. METHODS: cRNA 
      encoding wild type EAAT1 (SLC1A3) or EAAT2 (SLC1A2) was injected into Xenopus 
      oocytes without or with additional injection of cRNA encoding mTOR. Dual 
      electrode voltage clamp was performed in order to determine electrogenic 
      glutamate transport (IEAAT). EAAT2 protein abundance was determined utilizing 
      chemiluminescence. RESULTS: Appreciable IEAAT was observed in EAAT1 or EAAT2 
      expressing but not in water injected oocytes. IEAAT was significantly increased 
      by coexpression of mTOR. Coexpression of mTOR increased significantly the maximal 
      IEAAT in EAAT1 or EAAT2 expressing oocytes, without significantly modifying 
      affinity of the carriers. Moreover, coexpression of mTOR increased significantly 
      EAAT2 protein abundance in the cell membrane. CONCLUSIONS: The kinase mTOR 
      up-regulates the excitatory amino acid transporters EAAT1 and EAAT2.
CI  - (c) 2016 The Author(s) Published by S. Karger AG, Basel.
FAU - Abousaab, Abeer
AU  - Abousaab A
AD  - Department of Cardiology, Vascular Medicine and Physiology I, University of 
      Tuebingen, Tuebingen, Germany.
FAU - Uzcategui, Nestor Luis
AU  - Uzcategui NL
FAU - Elsir, Bhaeldin
AU  - Elsir B
FAU - Lang, Florian
AU  - Lang F
LA  - eng
PT  - Journal Article
DEP - 20161114
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Excitatory Amino Acid Transporter 1)
RN  - 0 (Excitatory Amino Acid Transporter 2)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Biological Transport
MH  - Cell Membrane/metabolism
MH  - Excitatory Amino Acid Transporter 1/*metabolism
MH  - Excitatory Amino Acid Transporter 2/*metabolism
MH  - Glutamic Acid/metabolism
MH  - Oocytes
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - *Up-Regulation
MH  - Xenopus laevis
EDAT- 2016/11/18 06:00
MHDA- 2017/02/07 06:00
CRDT- 2016/11/18 06:00
PHST- 2016/10/26 00:00 [accepted]
PHST- 2016/11/18 06:00 [entrez]
PHST- 2016/11/18 06:00 [pubmed]
PHST- 2017/02/07 06:00 [medline]
AID - 000452516 [pii]
AID - 10.1159/000452516 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2016;39(6):2492-2500. doi: 10.1159/000452516. Epub 2016 Nov 
      14.