PMID- 27859417
OWN - NLM
STAT- MEDLINE
DCOM- 20170131
LR  - 20220330
IS  - 1099-0844 (Electronic)
IS  - 0263-6484 (Linking)
VI  - 34
IP  - 8
DP  - 2016 Dec
TI  - RUNX3 inhibits laryngeal squamous cell carcinoma malignancy under the regulation 
      of miR-148a-3p/DNMT1 axis.
PG  - 597-605
LID - 10.1002/cbf.3233 [doi]
AB  - Laryngeal squamous cell carcinoma (LSCC) is a highly aggressive malignant cancer 
      and accounts for 1% to 2% of all malignancies diagnosed worldwide. Runt-related 
      transcription factor 3 (RUNX3), an important tumor suppressor, is known to 
      related to lymph node metastasis and the development of LSCC. However, the 
      biological roles and potential mechanisms RUNX3 expression was not well 
      understood. In this study, we reported that the RUNX3 was significantly 
      downregulated and highly methylated in LSCC compared with their matched normal. 
      The enforced expression of RUNX3 inhibited LSCC cell migration, invasion, and 
      proliferation, whereas the inhibition of RUNX3 did the opposite. We identified 
      that RUNX3 was regulated by miR-148a-3p and found that the expression level of 
      miR-148-3p was significantly decreased and positively related with the expression 
      of RUNX3 in LSCC. We also identified that DNA methyltransferase enzyme DNA 
      (cytosine-5-)-methyltransferase 1 (DNMT1) was targeted by miR-148a-3p in LSCC. 
      The knockdown of DNMT1 promoted the expression of RUNX3 and inhibited migration, 
      invasion, and proliferation in LSCC cells. In summary, our study demonstrated 
      that miR-148a-3p may regulate RUNX3 expression through the modulation of 
      DNMT1-dependent DNA methylation in LSCC, providing a novel target and a potential 
      therapeutic pathway against LSCC. LSCC is a highly aggressive malignant cancer 
      and accounts for 1% to 2% of all malignancies diagnosed worldwide. In this study, 
      we reported that RUNX3, an important tumor suppressor, was significantly 
      downregulated and highly methylated in LSCC compared with their matched normal. 
      The overexpression of RUNX3 inhibited LSCC cell migration, invasion, and 
      proliferation, whereas the inhibition of RUNX3 did the opposite. Moreover, RUNX3 
      was regulated by miR-148a-3p, which targeted DNA methyltransferase enzyme DNMT1 
      in LSCC cells. Therefore, miR-148a-3p may regulate RUNX3 expression through the 
      modulation of DNMT1-dependent DNA methylation in LSCC, providing a novel target 
      and a potential therapeutic pathway against LSCC.
CI  - Copyright (c) 2016 John Wiley & Sons, Ltd.
FAU - Jili, Su
AU  - Jili S
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The first Affiliated 
      Hospital, and College of Clinical Medicine of Henan University of Science and 
      Technology, Luoyang, 471003, China.
FAU - Eryong, Lu
AU  - Eryong L
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The first Affiliated 
      Hospital, and College of Clinical Medicine of Henan University of Science and 
      Technology, Luoyang, 471003, China.
FAU - Lijuan, Lu
AU  - Lijuan L
AD  - Department of Obstetrics and Gynecology, The first Affiliated Hospital, and 
      College of Clinical Medicine of Henan University of Science and Technology, 
      Luoyang, 471003, China.
FAU - Chao, Zhang
AU  - Chao Z
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The first Affiliated 
      Hospital, and College of Clinical Medicine of Henan University of Science and 
      Technology, Luoyang, 471003, China.
LA  - eng
PT  - Journal Article
DEP - 20161115
PL  - England
TA  - Cell Biochem Funct
JT  - Cell biochemistry and function
JID - 8305874
RN  - 0 (Core Binding Factor Alpha 3 Subunit)
RN  - 0 (MIRN148 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferase 1)
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases)
RN  - EC 2.1.1.37 (DNMT1 protein, human)
SB  - IM
MH  - Base Sequence
MH  - Carcinoma, Squamous Cell/*genetics/*pathology
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation
MH  - Core Binding Factor Alpha 3 Subunit/genetics/*metabolism
MH  - DNA (Cytosine-5-)-Methyltransferase 1
MH  - DNA (Cytosine-5-)-Methyltransferases/genetics/*metabolism
MH  - DNA Methylation/genetics
MH  - Disease Progression
MH  - Down-Regulation/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/*pathology
MH  - MicroRNAs/genetics/*metabolism
MH  - Neoplasm Invasiveness
MH  - Tumor Stem Cell Assay
OTO - NOTNLM
OT  - DNMT1
OT  - LSCC
OT  - RUNX3
OT  - methylation
OT  - miR-148a-3p
EDAT- 2016/11/20 06:00
MHDA- 2017/02/01 06:00
CRDT- 2016/11/19 06:00
PHST- 2016/08/22 00:00 [received]
PHST- 2016/10/07 00:00 [revised]
PHST- 2016/10/07 00:00 [accepted]
PHST- 2016/11/20 06:00 [pubmed]
PHST- 2017/02/01 06:00 [medline]
PHST- 2016/11/19 06:00 [entrez]
AID - 10.1002/cbf.3233 [doi]
PST - ppublish
SO  - Cell Biochem Funct. 2016 Dec;34(8):597-605. doi: 10.1002/cbf.3233. Epub 2016 Nov 
      15.