PMID- 27860425
OWN - NLM
STAT- MEDLINE
DCOM- 20171121
LR  - 20180226
IS  - 2047-2927 (Electronic)
IS  - 2047-2919 (Linking)
VI  - 5
IP  - 1
DP  - 2017 Jan
TI  - Centrally mediated ejaculatory response via sympathetic outflow in rats: role of 
      N-methyl-D-aspartic acid receptors in paraventricular nucleus.
PG  - 153-159
LID - 10.1111/andr.12274 [doi]
AB  - Ejaculation is mediated by a spinal generator, which integrates inputs related to 
      the sexual activity and coordinates sympathetic, parasympathetic, and motor 
      outflow. Previous clinical studies indicate that primary premature ejaculation is 
      related to the hyperactivity of the sympathetic nervous system. In this study, we 
      explored the roles of N-methyl-D-aspartic acid (NMDA) receptors in 
      paraventricular nucleus of the hypothalamus (PVN) on ejaculatory responses and 
      its potential mechanism in the rats. We found that microinjection of 0.20 nmol 
      NMDA into the PVN reduced the latency of intromission and facilitated ejaculation 
      during copulation. Moreover, delayed ejaculation and intromission were observed 
      after the rats were microinjected with NMDA receptor antagonist D 
      (-)-2-Amino-5-phosphonopentanoic acid (AP-5). However, we discovered that 
      microinjection of NMDA into PVN significantly increased baseline lumbar 
      splanchnic nerve activity (LSNA), and the NMDA dose was positively correlated 
      with the increased LSNA (r = 0.875, p = 0.04). Meanwhile, the plasma 
      norepinephrine level in rats injected with NMDA was much higher than that in rats 
      injected with saline (1453.4 +/- 136.4 pg/mL vs. 492.3 +/- 36.8 pg/mL, p < 0.01). 
      Additionally, AP-5 reduced the baseline LSNA and abrogated the enhancing activity 
      of NMDA in LSNA. Thus, we propose that NMDA receptors in PVN may facilitate 
      ejaculation through enhancing the activity of sympathetic system.
CI  - (c) 2016 American Society of Andrology and European Academy of Andrology.
FAU - Xia, J-D
AU  - Xia JD
AD  - Department of Urology, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, China.
FAU - Chen, J
AU  - Chen J
AD  - Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing 
      Medical University, Nanjing, China.
FAU - Sun, H-J
AU  - Sun HJ
AD  - Key Laboratory of Cardiovascular Disease and Molecular Intervention, Department 
      of Physiology, Nanjing Medical University, Nanjing, China.
FAU - Zhou, L-H
AU  - Zhou LH
AD  - Department of Urology, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, China.
FAU - Zhu, G-Q
AU  - Zhu GQ
AD  - Key Laboratory of Cardiovascular Disease and Molecular Intervention, Department 
      of Physiology, Nanjing Medical University, Nanjing, China.
FAU - Chen, Y
AU  - Chen Y
AD  - Department of Andrology, Nanjing Drum Tower Hospital, Nanjing Medical University, 
      Nanjing, China.
FAU - Dai, Y-T
AU  - Dai YT
AD  - Department of Andrology, Nanjing Drum Tower Hospital, Nanjing Medical University, 
      Nanjing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161116
PL  - England
TA  - Andrology
JT  - Andrology
JID - 101585129
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 118492-04-9 (2-amino-5-phosphono-3-pentenoic acid)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - 76726-92-6 (2-Amino-5-phosphonovalerate)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - 2-Amino-5-phosphonovalerate/analogs & derivatives/pharmacology
MH  - Animals
MH  - Ejaculation/drug effects/*physiology
MH  - Female
MH  - Male
MH  - Microinjections
MH  - N-Methylaspartate/pharmacology
MH  - Norepinephrine/blood
MH  - Paraventricular Hypothalamic Nucleus/drug effects/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
MH  - Sexual Behavior, Animal/drug effects/*physiology
MH  - Sympathetic Nervous System/drug effects/*metabolism
OTO - NOTNLM
OT  - ejaculation
OT  - paraventricular nucleus
OT  - sexual activity
OT  - sympathetic system nervous
EDAT- 2016/11/20 06:00
MHDA- 2017/11/29 06:00
CRDT- 2016/11/19 06:00
PHST- 2016/03/17 00:00 [received]
PHST- 2016/07/03 00:00 [revised]
PHST- 2016/07/19 00:00 [accepted]
PHST- 2016/11/20 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2016/11/19 06:00 [entrez]
AID - 10.1111/andr.12274 [doi]
PST - ppublish
SO  - Andrology. 2017 Jan;5(1):153-159. doi: 10.1111/andr.12274. Epub 2016 Nov 16.