PMID- 2786090
OWN - NLM
STAT- MEDLINE
DCOM- 19890627
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 63
IP  - 6
DP  - 1989 Jun
TI  - Identification of proteins specific for human herpesvirus 6-infected human T 
      cells.
PG  - 2835-40
AB  - Proteins specific for human herpesvirus 6 (HHV-6)-infected human T cells (HSB-2) 
      were examined by using polyclonal rabbit antibodies and monoclonal antibodies 
      against HHV-6-infected cells and human sera. More than 20 proteins and six 
      glycoproteins specific for HHV-6-infected cells were identified from 
      [35S]methionine- and [3H]glucosamine-labeled total-cell extracts. Polyclonal 
      rabbit antibodies immunoprecipitated 33 [35S]methionine-labeled HHV-6-specific 
      polypeptides with approximate molecular weights ranging from 180,000 to 31,000. 
      In immunoprecipitation and Western immunoblot reactions, a patient's serum also 
      recognized more than 30 HHV-6-specific proteins and seven glycoproteins. In 
      contrast, sera from individuals with high-titered antibodies against other human 
      herpesviruses reacted with fewer HHV-6-infected cell proteins, and only a 
      135,000-Mr polypeptide was prominent. Monoclonal antibodies to HHV-6-infected 
      cells reacted with single and multiple polypeptides specific for virus-infected 
      cells and immunoprecipitated three distinct sets of glycoproteins, which were 
      designated gp105k and gp82k, gp116k, gp64k, and gp54k, and gp102k.
FAU - Balachandran, N
AU  - Balachandran N
AD  - Department of Microbiology, Molecular Genetics and Immunology, University of 
      Kansas Medical Center, Kansas City 66103.
FAU - Amelse, R E
AU  - Amelse RE
FAU - Zhou, W W
AU  - Zhou WW
FAU - Chang, C K
AU  - Chang CK
LA  - eng
GR  - AI-24224/AI/NIAID NIH HHS/United States
GR  - S07-RR0573/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Immune Sera)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Antibodies, Monoclonal/immunology
MH  - Antibodies, Viral/immunology
MH  - Antigens, Viral/analysis/immunology
MH  - Blotting, Western
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Herpesviridae/*analysis/immunology/physiology
MH  - Humans
MH  - Immune Sera/immunology
MH  - Precipitin Tests
MH  - T-Lymphocytes/*microbiology
MH  - Viral Proteins/*analysis/immunology
PMC - PMC250791
EDAT- 1989/06/01 00:00
MHDA- 1989/06/01 00:01
PMCR- 1989/06/01
CRDT- 1989/06/01 00:00
PHST- 1989/06/01 00:00 [pubmed]
PHST- 1989/06/01 00:01 [medline]
PHST- 1989/06/01 00:00 [entrez]
PHST- 1989/06/01 00:00 [pmc-release]
AID - 10.1128/JVI.63.6.2835-2840.1989 [doi]
PST - ppublish
SO  - J Virol. 1989 Jun;63(6):2835-40. doi: 10.1128/JVI.63.6.2835-2840.1989.