PMID- 27862629
OWN - NLM
STAT- MEDLINE
DCOM- 20171219
LR  - 20180331
IS  - 1549-4918 (Electronic)
IS  - 1066-5099 (Linking)
VI  - 35
IP  - 3
DP  - 2017 Mar
TI  - Secreted Ectodomain of Sialic Acid-Binding Ig-Like Lectin-9 and Monocyte 
      Chemoattractant Protein-1 Synergistically Regenerate Transected Rat Peripheral 
      Nerves by Altering Macrophage Polarity.
PG  - 641-653
LID - 10.1002/stem.2534 [doi]
AB  - Peripheral nerves (PNs) exhibit remarkable self-repairing reparative activity 
      after a simple crush or cut injury. However, the neuronal transection involving a 
      nerve gap overwhelms their repairing activity and causes persistent paralysis. 
      Here, we show that an implantation of the serum-free conditioned medium from stem 
      cells from human exfoliated deciduous teeth (SHED-CM) immersed in a collagen 
      sponge into the nerve gap formed by rat facial nerves transection restored the 
      neurological function. In contrast, SHED-CM specifically depleted of a set of 
      anti-inflammatory M2 macrophage inducers, monocyte chemoattractant protein-1 
      (MCP-1) and the secreted ectodomain of sialic acid-binding Ig-like lectin-9 
      (sSiglec-9) lost the ability to restore neurological function in this model. 
      Notably, the combination of MCP-1 and sSiglec-9 induced the polarization of M2 
      macrophages in vitro, resulting in the expression of multiple trophic factors 
      that enhanced proliferation, migration, and differentiation of Schwann cells, 
      blood vessel formation, and nerve fiber extension. Furthermore, the implantation 
      of a collagen graft containing MCP-1/sSiglec-9 into the nerve gap induced 
      anti-inflammatory M2 macrophage polarization, generated a Schwann-cell bridge 
      instead of fibrotic scar, induced axonal regrowth, and restored nerve function. 
      The specific elimination of M2 macrophages by Mannosylated-Clodrosome suppressed 
      the MCP-1/sSiglec-9-mediated neurological recovery. Taken together, our data 
      suggest that MCP-1/sSiglec-9 regenerates PNs by inducing tissue-repairing M2 
      macrophages and may provide therapeutic benefits for severe peripheral nerve 
      injuries. Stem Cells 2017;35:641-653.
CI  - (c) 2016 AlphaMed Press.
FAU - Kano, Fumiya
AU  - Kano F
AD  - Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School 
      of Medicine, Showa-ku, Nagoya, Japan.
FAU - Matsubara, Kohki
AU  - Matsubara K
AD  - Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School 
      of Medicine, Showa-ku, Nagoya, Japan.
FAU - Ueda, Minoru
AU  - Ueda M
AD  - Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School 
      of Medicine, Showa-ku, Nagoya, Japan.
FAU - Hibi, Hideharu
AU  - Hibi H
AD  - Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School 
      of Medicine, Showa-ku, Nagoya, Japan.
FAU - Yamamoto, Akihito
AU  - Yamamoto A
AD  - Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School 
      of Medicine, Showa-ku, Nagoya, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161209
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Sialic Acid Binding Immunoglobulin-like Lectins)
RN  - 0 (Siglec-9 protein, rat)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Movement
MH  - *Cell Polarity
MH  - Cell Proliferation
MH  - Chemokine CCL2/*metabolism
MH  - Child
MH  - Facial Nerve/physiopathology
MH  - Female
MH  - Ganglia, Spinal/metabolism
MH  - Humans
MH  - Inflammation/pathology
MH  - Macrophages/metabolism/*pathology
MH  - Nerve Regeneration
MH  - Neuronal Outgrowth
MH  - Peripheral Nerves/*pathology/physiopathology
MH  - Rats, Sprague-Dawley
MH  - Recovery of Function
MH  - Schwann Cells
MH  - Sialic Acid Binding Immunoglobulin-like Lectins/*metabolism
MH  - Signal Transduction
MH  - Stem Cells/metabolism
MH  - Tooth, Deciduous/cytology
OTO - NOTNLM
OT  - Dental pulp stem cells
OT  - Macrophages
OT  - Monocyte Chemoattractant Protein-1
OT  - Peripheral nerve injury
OT  - Sialic acid-binding Ig-like lectin-9
EDAT- 2016/11/20 06:00
MHDA- 2017/12/20 06:00
CRDT- 2016/11/19 06:00
PHST- 2016/06/01 00:00 [received]
PHST- 2016/09/30 00:00 [revised]
PHST- 2016/10/10 00:00 [accepted]
PHST- 2016/11/20 06:00 [pubmed]
PHST- 2017/12/20 06:00 [medline]
PHST- 2016/11/19 06:00 [entrez]
AID - 10.1002/stem.2534 [doi]
PST - ppublish
SO  - Stem Cells. 2017 Mar;35(3):641-653. doi: 10.1002/stem.2534. Epub 2016 Dec 9.