PMID- 27863080
OWN - NLM
STAT- MEDLINE
DCOM- 20171002
LR  - 20180808
IS  - 1862-8354 (Electronic)
IS  - 1862-8346 (Linking)
VI  - 11
IP  - 3-4
DP  - 2017 Mar
TI  - Screening for potential serum-based proteomic biomarkers for human type 2 
      diabetes mellitus using MALDI-TOF MS.
LID - 10.1002/prca.201600079 [doi]
AB  - BACKGROUND: Type 2 diabetes mellitus (T2DM) is a complex, pandemic disease 
      contributing towards the global burden of health issues. To date, there are no 
      simple clinical tests for the early detection of T2DM. METHOD: To identify 
      potential peptide biomarkers for such applications, 406 sera of T2DM patients (n 
      = 206) and healthy controls (n = 200) are analyzed by using MALDI-TOF MS with a 
      cross-sectional case-control design. RESULT: Six peptides (peaks m/z 1452.9, 
      1692.8, 1946.0, 2115.1, 2211.0 and 4053.6) are identified as candidate biomarkers 
      for T2DM. A diagnostic model constructed with six peptides is able to 
      discriminate T2DM patients from healthy controls, with an accuracy of 82.20%, 
      sensitivity of 82.50%, and specificity of 77.80% in the validation set. Peptide 
      peaks m/z 1452.9 and 1692.8 are identified as fragments of the complement C3f, 
      while peptide peaks m/z 1946.0, 2115.1, and 2211.0 are identified as the 
      fragments of kininogen 1 isoform 1 precursor. CONCLUSION: This study reinforces 
      proteomic analyses as a potential technique for defining significant clinical 
      peptide biomarkers, providing a simple and convenient diagnostic model for T2DM 
      in clinical examination.
CI  - (c) 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Meng, Qiutao
AU  - Meng Q
AD  - Department of Endocrinology, Chinese PLA General Hospital, Beijing, China.
FAU - Ge, Siqi
AU  - Ge S
AD  - Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital 
      Medical University, Beijing, China.
AD  - School of Medical and Health Sciences, Edith Cowan University, Perth, Australia.
FAU - Yan, Wenhua
AU  - Yan W
AD  - Department of Endocrinology, Chinese PLA General Hospital, Beijing, China.
FAU - Li, Ruisheng
AU  - Li R
AD  - Research and Technology Service Center, Chinese PLA 302 Hospital, Beijing, China.
FAU - Dou, Jingtao
AU  - Dou J
AD  - Department of Endocrinology, Chinese PLA General Hospital, Beijing, China.
FAU - Wang, Haibing
AU  - Wang H
AD  - Department of Endocrinology, Chinese PLA General Hospital, Beijing, China.
FAU - Wang, Baoan
AU  - Wang B
AD  - Department of Endocrinology, Chinese PLA General Hospital, Beijing, China.
FAU - Ma, Qingwei
AU  - Ma Q
AD  - Bioyong (Beijing) Technology Co., Ltd., Beijing, China.
FAU - Zhou, Yong
AU  - Zhou Y
AD  - Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital 
      Medical University, Beijing, China.
FAU - Song, Manshu
AU  - Song M
AD  - Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital 
      Medical University, Beijing, China.
FAU - Yu, Xinwei
AU  - Yu X
AD  - Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital 
      Medical University, Beijing, China.
AD  - School of Medical and Health Sciences, Edith Cowan University, Perth, Australia.
FAU - Wang, Hao
AU  - Wang H
AD  - Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital 
      Medical University, Beijing, China.
FAU - Yang, Xinghua
AU  - Yang X
AD  - Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital 
      Medical University, Beijing, China.
FAU - Liu, Fen
AU  - Liu F
AD  - Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital 
      Medical University, Beijing, China.
FAU - Alzain, Mohamed Ali
AU  - Alzain MA
AD  - Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital 
      Medical University, Beijing, China.
FAU - Yan, Yuxiang
AU  - Yan Y
AD  - Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital 
      Medical University, Beijing, China.
FAU - Zhang, Ling
AU  - Zhang L
AD  - Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital 
      Medical University, Beijing, China.
FAU - Wu, Lijuan
AU  - Wu L
AD  - Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital 
      Medical University, Beijing, China.
FAU - Zhao, Feifei
AU  - Zhao F
AD  - Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital 
      Medical University, Beijing, China.
FAU - He, Yan
AU  - He Y
AD  - Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital 
      Medical University, Beijing, China.
FAU - Guo, Xiuhua
AU  - Guo X
AD  - Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital 
      Medical University, Beijing, China.
FAU - Chen, Feng
AU  - Chen F
AD  - Central of Laboratory, Peking University School and Hospital of Stomatology, 
      Beijing, China.
FAU - Xu, Weizhuo
AU  - Xu W
AD  - School of Life Science and Biopharmaceuticals, Shenyang Pharmaceutical 
      University, Shenyang, China.
FAU - Garcia, Monique
AU  - Garcia M
AD  - School of Medical and Health Sciences, Edith Cowan University, Perth, Australia.
FAU - Menon, Desmond
AU  - Menon D
AD  - School of Medical and Health Sciences, Edith Cowan University, Perth, Australia.
FAU - Wang, Youxin
AU  - Wang Y
AD  - Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital 
      Medical University, Beijing, China.
FAU - Mu, Yiming
AU  - Mu Y
AD  - Department of Endocrinology, Chinese PLA General Hospital, Beijing, China.
FAU - Wang, Wei
AU  - Wang W
AD  - Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital 
      Medical University, Beijing, China.
AD  - School of Medical and Health Sciences, Edith Cowan University, Perth, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161130
PL  - Germany
TA  - Proteomics Clin Appl
JT  - Proteomics. Clinical applications
JID - 101298608
RN  - 0 (Biomarkers)
SB  - IM
MH  - Biomarkers/blood
MH  - Cross-Sectional Studies
MH  - Diabetes Mellitus, Type 2/*blood/diagnosis
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - *Proteomics
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
OTO - NOTNLM
OT  - MALDI-TOF MS
OT  - Predictive models
OT  - Serum peptide biomarkers
OT  - Type 2 diabetes mellitus
EDAT- 2016/11/20 06:00
MHDA- 2017/10/03 06:00
CRDT- 2016/11/19 06:00
PHST- 2016/05/25 00:00 [received]
PHST- 2016/10/04 00:00 [revised]
PHST- 2016/11/04 00:00 [accepted]
PHST- 2016/11/20 06:00 [pubmed]
PHST- 2017/10/03 06:00 [medline]
PHST- 2016/11/19 06:00 [entrez]
AID - 10.1002/prca.201600079 [doi]
PST - ppublish
SO  - Proteomics Clin Appl. 2017 Mar;11(3-4). doi: 10.1002/prca.201600079. Epub 2016 
      Nov 30.