PMID- 27863548
OWN - NLM
STAT- MEDLINE
DCOM- 20170424
LR  - 20171116
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 182
DP  - 2016 Dec
TI  - Impact of in vitro treatments of physiological levels of estradiol and 
      progesterone observed in pregnancy on bovine monocyte-derived dendritic cell 
      differentiation and maturation.
PG  - 37-42
LID - S0165-2427(16)30181-7 [pii]
LID - 10.1016/j.vetimm.2016.09.007 [doi]
AB  - The specific factors which regulate differentiation and maturation of dendritic 
      cells in bovine pregnancy remain unclear. We evaluated the influence of 
      physiologically relevant in vitro treatments of progesterone (PG) and estradiol 
      (E2) observed in late pregnancy on the differentiation and maturation of CD14+ 
      monocyte-derived dendritic cell (moDC) from non-pregnant, lactating dairy cows 
      (n=7). We found that moDC differentiated in the presence of both E2 and PG had 
      impaired E. coli-induced phenotypic maturation, specifically a significant 
      reduction in CD80 and MHC II expression. Contrary to our previous work 
      characterizing moDC from late gestating dairy cattle, we did not observe an 
      increase in CD14 expression relative to the untreated control; this increase was 
      only observed in the current data in the dexamethasone-treated moDC. The moDC 
      treated with a combination of both E2 and PG had significantly greater 
      upregulation of anti-inflammatory cytokine IL-10 relative to the untreated 
      control, but TNFalpha production was not suppressed; only dexamethasone-treated moDC 
      showed abrogated TNFalpha production. These data suggest moDC may be regulated by E2 
      and PG to hinder phenotypic maturation and regulate inflammatory responses. 
      Pregnancy-associated hormone profiles appear to be involved in the generation of 
      maternal immune tolerance in pregnancy. These hormone-facilitated changes to moDC 
      in pregnancy may also impede optimal immune responses to both invading pathogens 
      and routine vaccinations administered in late gestation through limited antigen 
      presentation and increased anti-inflammatory cytokine production. These results 
      provide insight into maternal immune modulation and elucidate potential immune 
      changes necessary to facilitate bovine pregnancy.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Pomeroy, Brianna
AU  - Pomeroy B
AD  - S3 119, Schurman Hall, College of Veterinary Medicine, Cornell University, 
      Ithaca, NY 14850, USA; Department of Population Medicine & Diagnostic Sciences, 
      College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. 
      Electronic address: bjp62@cornell.edu.
FAU - Klaessig, Suzanne
AU  - Klaessig S
AD  - Department of Population Medicine & Diagnostic Sciences, College of Veterinary 
      Medicine, Cornell University, Ithaca, NY 14853, USA. Electronic address: 
      skc10@cornell.edu.
FAU - Schukken, Ynte
AU  - Schukken Y
AD  - Department of Population Medicine & Diagnostic Sciences, College of Veterinary 
      Medicine, Cornell University, Ithaca, NY 14853, USA; GD Animal Health, Deventer, 
      The Netherlands; Department of Animal Sciences, Wageningen University, 
      Wageningen,The Netherlands. Electronic address: Y.Schukken@gddeventer.com.
LA  - eng
PT  - Journal Article
DEP - 20160922
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (B7-1 Antigen)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 130068-27-8 (Interleukin-10)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - B7-1 Antigen/metabolism
MH  - Cattle/*immunology
MH  - Cell Differentiation/drug effects/immunology
MH  - Dendritic Cells/cytology/drug effects/*immunology
MH  - Estradiol/administration & dosage/*immunology
MH  - Female
MH  - Histocompatibility Antigens Class II/metabolism
MH  - In Vitro Techniques
MH  - Interleukin-10/metabolism
MH  - Monocytes/cytology/drug effects/immunology
MH  - Pregnancy
MH  - Pregnancy, Animal/*immunology
MH  - Progesterone/administration & dosage/*immunology
OTO - NOTNLM
OT  - Bovine
OT  - Estradiol
OT  - Monocyte-derived dendritic cell
OT  - Progesterone
EDAT- 2016/11/20 06:00
MHDA- 2017/04/25 06:00
CRDT- 2016/11/20 06:00
PHST- 2016/07/13 00:00 [received]
PHST- 2016/09/07 00:00 [revised]
PHST- 2016/09/21 00:00 [accepted]
PHST- 2016/11/20 06:00 [entrez]
PHST- 2016/11/20 06:00 [pubmed]
PHST- 2017/04/25 06:00 [medline]
AID - S0165-2427(16)30181-7 [pii]
AID - 10.1016/j.vetimm.2016.09.007 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2016 Dec;182:37-42. doi: 10.1016/j.vetimm.2016.09.007. 
      Epub 2016 Sep 22.