PMID- 27864781
OWN - NLM
STAT- MEDLINE
DCOM- 20170619
LR  - 20170619
IS  - 1559-0291 (Electronic)
IS  - 0273-2289 (Linking)
VI  - 182
IP  - 1
DP  - 2017 May
TI  - Isolation, Identification and High-Throughput Screening of Neutral Lipid 
      Producing Indigenous Microalgae from South African Aquatic Habitats.
PG  - 382-399
LID - 10.1007/s12010-016-2333-z [doi]
AB  - Exploring indigenous microalgae capable of producing significant amounts of 
      neutral lipids through high-throughput screening is crucial for sustainable 
      biodiesel production. In this study, 31 indigenous microalgal strains were 
      isolated from diverse aquatic habitats in KwaZulu-Natal, South Africa. Eight 
      superior lipid-producing strains were selected for further analysis, based on 
      Nile red fluorescence microscopy screening. The microalgal isolates were 
      identified to belong to the genera Chlorella, Neochloris and Chlamydomonas via 
      morpho-taxonomic and molecular approach by 18S rRNA gene sequencing. Chlorella 
      vulgaris PH2 had the highest specific growth rate (mu) and lowest doubling time of 
      0.24 day(-1) and 2.89 +/- 0.05 day(-1), respectively. Chlorella vulgaris T4 had the 
      highest biomass productivity of 35.71 +/- 0.03 mg L(-1)day(-1). Chlorella vulgaris 
      PH2 had the highest lipid content of 34.28 +/- 0.47 and 38 +/- 9.2% (dcw) as 
      determined by gravimetric analysis and the sulfo-phospho-vanillin (SPV) method, 
      respectively. Chlorella vulgaris PH2 exhibited a high content of saturated fatty 
      acids, while Chlorella sp. T4 exhibited a high total content of saturated and 
      monounsaturated fatty acids with a low content of polyunsaturated fatty acids. 
      The preponderance of neutral lipids suggests that Chlorella sp. T4 is a suitable 
      candidate for biomass feedstock for biodiesel production.
FAU - Gumbi, S T
AU  - Gumbi ST
AD  - Discipline of Microbiology, School of Life Sciences, College of Agriculture 
      Engineering and Sciences, University of KwaZulu-Natal (Westville Campus), Private 
      Bag X54001, Durban, 4000, South Africa.
FAU - Majeke, B M
AU  - Majeke BM
AD  - Discipline of Microbiology, School of Life Sciences, College of Agriculture 
      Engineering and Sciences, University of KwaZulu-Natal (Westville Campus), Private 
      Bag X54001, Durban, 4000, South Africa.
FAU - Olaniran, A O
AU  - Olaniran AO
AD  - Discipline of Microbiology, School of Life Sciences, College of Agriculture 
      Engineering and Sciences, University of KwaZulu-Natal (Westville Campus), Private 
      Bag X54001, Durban, 4000, South Africa.
FAU - Mutanda, T
AU  - Mutanda T
AD  - Discipline of Microbiology, School of Life Sciences, College of Agriculture 
      Engineering and Sciences, University of KwaZulu-Natal (Westville Campus), Private 
      Bag X54001, Durban, 4000, South Africa. mutanda@ukzn.ac.za.
LA  - eng
PT  - Journal Article
DEP - 20161118
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - 0 (Biofuels)
RN  - 0 (Fatty Acids)
RN  - 0 (Fatty Acids, Monounsaturated)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (RNA, Ribosomal, 18S)
SB  - IM
MH  - Aquatic Organisms
MH  - Biofuels
MH  - Biomass
MH  - Chlamydomonas/chemistry/genetics/*metabolism
MH  - Chlorella/chemistry/genetics/*metabolism
MH  - Fatty Acids/biosynthesis/*isolation & purification
MH  - Fatty Acids, Monounsaturated/*isolation & purification/metabolism
MH  - Fatty Acids, Unsaturated/biosynthesis/*isolation & purification
MH  - High-Throughput Screening Assays
MH  - Microalgae/chemistry/genetics/*metabolism
MH  - RNA, Ribosomal, 18S/genetics
MH  - South Africa
OTO - NOTNLM
OT  - Biodiesel
OT  - Bioprospect
OT  - Chlorella sp.
OT  - Fatty acid methyl esters
OT  - GC-MS
OT  - Indigenous
OT  - Lipid profile
OT  - Microalgae
EDAT- 2016/11/20 06:00
MHDA- 2017/06/20 06:00
CRDT- 2016/11/20 06:00
PHST- 2016/09/01 00:00 [received]
PHST- 2016/11/10 00:00 [accepted]
PHST- 2016/11/20 06:00 [pubmed]
PHST- 2017/06/20 06:00 [medline]
PHST- 2016/11/20 06:00 [entrez]
AID - 10.1007/s12010-016-2333-z [pii]
AID - 10.1007/s12010-016-2333-z [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 2017 May;182(1):382-399. doi: 10.1007/s12010-016-2333-z. 
      Epub 2016 Nov 18.