PMID- 27864985
OWN - NLM
STAT- MEDLINE
DCOM- 20171010
LR  - 20171010
IS  - 1365-2265 (Electronic)
IS  - 0300-0664 (Linking)
VI  - 86
IP  - 4
DP  - 2017 Apr
TI  - Telomeric repeat-containing RNA (TERRA) related to polycystic ovary syndrome 
      (PCOS).
PG  - 552-559
LID - 10.1111/cen.13283 [doi]
AB  - CONTEXT: Telomeric repeat-containing RNA (TERRA) participates in the regulation 
      of telomere length, and leucocyte telomere length (LTL) plays an important role 
      in the pathophysiology of polycystic ovary syndrome (PCOS), but little is known 
      about the role of TERRA in PCOS. OBJECTIVE: To evaluate the role of TERRA and 
      peripheral blood LTL in PCOS. DESIGN AND PARTICIPANTS: Forty women with PCOS and 
      35 healthy women without PCOS were recruited. A prospective case-control study 
      was performed. MEASUREMENTS: RNA fluorescence in situ hybridization (FISH) was 
      used to detect TERRA expression in peripheral blood leucocyte. Quantitative PCR 
      was used to measure TERRA expression and the mean LTL in the PCOS and control 
      groups. We analysed the association between related clinical parameters and the 
      age-adjusted ratio of the telomere repeat length (T/S ratio) or TERRA. RESULTS: 
      Telomeric repeat-containing RNA was expressed in human peripheral blood 
      leucocytes, and the signal was abolished after culture with RNase A. The 
      age-adjusted LTLs were significantly longer in the PCOS group than in the control 
      group (P < 0.01). The age-adjusted TERRA level was significantly lower in the 
      PCOS group than in the control group (P < 0.01). Testosterone (TTE) was related 
      positively to LTL and negatively to TERRA in the PCOS group (r = 0.532, P = 
      0.002; r = -0.477, P = 0.017). CONCLUSION: We found TERRA expression in human 
      peripheral blood leucocytes, and LTLs were positively associated with PCOS. TERRA 
      and testosterone play an important role in the LTL regulation in PCOS.
CI  - (c) 2016 John Wiley & Sons Ltd.
FAU - Wang, Caiqin
AU  - Wang C
AD  - Key Laboratory of Reproductive Genetics (Zhejiang University), Ministry of 
      Education, China, Women's Hospital, School of Medicine, Zhejiang University, 
      Hangzhou, China.
FAU - Shen, Fengxian
AU  - Shen F
AD  - Key Laboratory of Reproductive Genetics (Zhejiang University), Ministry of 
      Education, China, Women's Hospital, School of Medicine, Zhejiang University, 
      Hangzhou, China.
FAU - Zhu, Yuning
AU  - Zhu Y
AD  - Key Laboratory of Reproductive Genetics (Zhejiang University), Ministry of 
      Education, China, Women's Hospital, School of Medicine, Zhejiang University, 
      Hangzhou, China.
FAU - Fang, Yuying
AU  - Fang Y
AD  - Key Laboratory of Reproductive Genetics (Zhejiang University), Ministry of 
      Education, China, Women's Hospital, School of Medicine, Zhejiang University, 
      Hangzhou, China.
FAU - Lu, Shiming
AU  - Lu S
AD  - Key Laboratory of Reproductive Genetics (Zhejiang University), Ministry of 
      Education, China, Women's Hospital, School of Medicine, Zhejiang University, 
      Hangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20161219
PL  - England
TA  - Clin Endocrinol (Oxf)
JT  - Clinical endocrinology
JID - 0346653
RN  - 3XMK78S47O (Testosterone)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - Female
MH  - Humans
MH  - Leukocytes/metabolism
MH  - Polycystic Ovary Syndrome/*etiology/genetics
MH  - Prospective Studies
MH  - RNA/*physiology
MH  - Telomere/metabolism/*physiology/ultrastructure
MH  - Testosterone/blood
MH  - Young Adult
EDAT- 2016/11/20 06:00
MHDA- 2017/10/11 06:00
CRDT- 2016/11/20 06:00
PHST- 2016/06/22 00:00 [received]
PHST- 2016/09/16 00:00 [revised]
PHST- 2016/11/01 00:00 [revised]
PHST- 2016/11/09 00:00 [accepted]
PHST- 2016/11/20 06:00 [pubmed]
PHST- 2017/10/11 06:00 [medline]
PHST- 2016/11/20 06:00 [entrez]
AID - 10.1111/cen.13283 [doi]
PST - ppublish
SO  - Clin Endocrinol (Oxf). 2017 Apr;86(4):552-559. doi: 10.1111/cen.13283. Epub 2016 
      Dec 19.