PMID- 27865352
OWN - NLM
STAT- MEDLINE
DCOM- 20180108
LR  - 20211204
IS  - 1879-0739 (Electronic)
IS  - 0271-5317 (Linking)
VI  - 36
IP  - 10
DP  - 2016 Oct
TI  - Plum polyphenols inhibit colorectal aberrant crypt foci formation in rats: 
      potential role of the miR-143/protein kinase B/mammalian target of rapamycin 
      axis.
PG  - 1105-1113
LID - S0271-5317(16)30095-1 [pii]
LID - 10.1016/j.nutres.2016.06.008 [doi]
AB  - The nutritional prevention of aberrant crypt foci by polyphenols may be a crucial 
      step to dietary cancer prevention. The objective of this study was to determine 
      the underlying mechanisms that contribute to the anti-inflammatory and 
      antitumorigenic properties of plum (Prunus salicina L.) polyphenols, including 
      chlorogenic acid and neochlorogenic acid, in azoxymethane (AOM)-treated rats. The 
      hypothesis was that plum polyphenolics suppress AOM-induced aberrant crypt foci 
      formation through alterations in the protein kinase B (AKT)/mammalian target of 
      rapamycin (mTOR) pathway and relative micro-RNA expressions. Sprague-Dawley rats 
      (n=10/group) received plum beverage (1346mg gallic acid equivalents/L) or a 
      control beverage ad libitum for 10 weeks with subcutaneous injections of AOM 
      (15mg/kg) at weeks 2 and 3. Results show that the consumption of the plum 
      beverage decreased the number of dysplastic aberrant crypt foci by 48% (P<.05) 
      and lowered proliferation of mucosal cells by 24% (P<.05). The plum beverage 
      decreased the activity of glutathione peroxidase, superoxide dismutase, and 
      catalase in mucosal scrapings, as well as the superoxide dismutase activity in 
      serum. The results were accompanied by a down-regulation of proinflammatory 
      enzymes nuclear factor kappaB, nitric oxide synthase, cyclooxygenase-2, and vascular 
      cell adhesion molecule 1 messenger RNA. Plum inhibited the expression of AKT and 
      mTOR messenger RNA, phosphorylated AKT, mTOR, and hypoxia-inducible factor-1alpha 
      protein levels, and the ratio of the phosphorylated/total protein expression of 
      mTOR. Also, the plum beverage increased the expression of miR-143, which is 
      involved in the regulation of AKT. These results suggest that plum polyphenols 
      may exhibit a chemopreventive potential against colon carcinogenesis by impacting 
      the AKT/mTOR pathway and miR-143.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Banerjee, Nivedita
AU  - Banerjee N
AD  - Interdisciplinary Program of Toxicology, Texas A&M University, College Station, 
      TX 77843, USA.
FAU - Kim, Hyemee
AU  - Kim H
AD  - Department of Nutrition and Food Science, Texas A&M University, College Station, 
      TX 77843, USA.
FAU - Talcott, Stephen T
AU  - Talcott ST
AD  - Department of Nutrition and Food Science, Texas A&M University, College Station, 
      TX 77843, USA.
FAU - Turner, Nancy D
AU  - Turner ND
AD  - Interdisciplinary Program of Toxicology, Texas A&M University, College Station, 
      TX 77843, USA; Department of Nutrition and Food Science, Texas A&M University, 
      College Station, TX 77843, USA.
FAU - Byrne, David H
AU  - Byrne DH
AD  - Department of Horticulture, Texas A&M University, College Station, TX 77843, USA.
FAU - Mertens-Talcott, Susanne U
AU  - Mertens-Talcott SU
AD  - Department of Nutrition and Food Science, Texas A&M University, College Station, 
      TX 77843, USA; Department of Veterinary Physiology & Pharmacology, Texas A&M 
      University, College Station, TX 77843, USA. Electronic address: 
      smtalcott@tamu.edu.
LA  - eng
PT  - Journal Article
DEP - 20160614
PL  - United States
TA  - Nutr Res
JT  - Nutrition research (New York, N.Y.)
JID - 8303331
RN  - 0 (Anticarcinogenic Agents)
RN  - 0 (Inflammation Mediators)
RN  - 0 (MIRN143 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (Plant Extracts)
RN  - 0 (Polyphenols)
RN  - 058C04BGYI (Quinic Acid)
RN  - 318ADP12RI (Chlorogenic Acid)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - MO0N1J0SEN (Azoxymethane)
RN  - O4601UER1Z (5'-O-caffeoylquinic acid)
SB  - IM
MH  - Aberrant Crypt Foci/chemically induced/*prevention & control
MH  - Animals
MH  - Anticarcinogenic Agents/pharmacology/therapeutic use
MH  - Azoxymethane
MH  - Cell Proliferation
MH  - Chlorogenic Acid/analogs & derivatives/pharmacology/therapeutic use
MH  - Colon/drug effects/pathology
MH  - Colonic Neoplasms/*metabolism/prevention & control
MH  - Diet
MH  - Inflammation Mediators/metabolism
MH  - Intestinal Mucosa/drug effects/pathology
MH  - Male
MH  - MicroRNAs/*metabolism
MH  - Phosphorylation
MH  - Plant Extracts/pharmacology
MH  - Polyphenols/*pharmacology/therapeutic use
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - Prunus domestica/*chemistry
MH  - Quinic Acid/analogs & derivatives/pharmacology/therapeutic use
MH  - Rats, Sprague-Dawley
MH  - TOR Serine-Threonine Kinases/*metabolism
OTO - NOTNLM
OT  - Chlorogenic acid
OT  - Colon cancer
OT  - Inflammation
OT  - Plum
OT  - Rat
OT  - mTOR
OT  - miR-143
EDAT- 2016/11/21 06:00
MHDA- 2018/01/09 06:00
CRDT- 2016/11/21 06:00
PHST- 2016/01/25 00:00 [received]
PHST- 2016/05/18 00:00 [revised]
PHST- 2016/06/10 00:00 [accepted]
PHST- 2016/11/21 06:00 [entrez]
PHST- 2016/11/21 06:00 [pubmed]
PHST- 2018/01/09 06:00 [medline]
AID - S0271-5317(16)30095-1 [pii]
AID - 10.1016/j.nutres.2016.06.008 [doi]
PST - ppublish
SO  - Nutr Res. 2016 Oct;36(10):1105-1113. doi: 10.1016/j.nutres.2016.06.008. Epub 2016 
      Jun 14.