PMID- 27868407 OWN - NLM STAT- MEDLINE DCOM- 20170925 LR - 20230805 IS - 1008-9292 (Print) IS - 1008-9292 (Linking) VI - 45 IP - 4 DP - 2016 May 25 TI - [Effect of DJ-1 silencing by RNA interference on growth of xenografted human laryngeal squamous cell carcinoma Hep-2 cells in nude mice]. PG - 349-355 AB - Objective: To investigate the effect of silencing DJ-1 on xenografted human laryngeal squamous cell carcinoma (LSCC) Hep-2 cells in nude mice. Methods: Xenograft model of human LSCC was established by subcutaneous transplantation of Hep-2 cells in 24 nude mice. The LSCC-bearing nude mice were randomly divided into 3 groups (n=8 in each):DJ-1 siRNA low dose group and DJ-1 siRNA high dose group were injected in tumors with 20 mug of DJ-1 siRNA or 40 mug of DJ-1 siRNA in 50 muL, respectively; control group was injected with 5% glucose solution in 50 muL, twice a week for 3 weeks. The weight and size of tumors were measured before injection. The animals were sacrificed 48 h after the final treatment, and the tumors were harvested and weighed. The apoptosis and proliferation of tumor cells were determined; the expressions of Caspase-3 and Ki-67 in tumor specimens were detected with immunohistochemistry. The expression of DJ-1, PTEN, survivin mRNA and protein in tumor tissues were detected by RT-PCR and Western blotting, respectively. Results: Tumor weight in low dose group[(0.66+/-0.15)g] and high dose group[(0.48+/-0.11)g] were significantly lower than that in control group[(0.83+/-0.16)g, all P<0.05]. The inhibition rates of low dose group and high dose group were (20.48+/-0.18)% and (42.16+/-0.13)%, respectively. Immunohistochemistry showed that the expression of Caspase-3 was increased and Ki-67 was reduced in tumor specimens, compared with the control group (all P<0.05). RT-PCR and Western blot results showed that in low dose group and high dose group the mRNA and protein expression of DJ-1 and survivin significantly decreased (all P<0.05), while PTEN mRNA and protein content increased (all P<0.05). Conclusion: High dose DJ-1 siRNA can inhibit the tumor growth in human LSCC xenograft nude mouse model, which indicates that down-regulating DJ-1 and survivin, and up-regulating PTEN expression may lead to blockage of PI3K-PKB/Akt signaling pathway and promoting tumor cell apoptosis. FAU - Shen, Zhisen AU - Shen Z AD - Department of Otorhinolaryngology, Ningbo Medical Center Lihuili Hospital, Ningbo 315040, China. FAU - Deng, Hongxia AU - Deng H AD - Department of Otorhinolaryngology, Ningbo Medical Center Lihuili Hospital, Ningbo 315040, China. FAU - Ye, Dong AU - Ye D AD - Department of Otorhinolaryngology, Ningbo Medical Center Lihuili Hospital, Ningbo 315040, China. FAU - Zhang, Jian AU - Zhang J AD - Department of Otorhinolaryngology, Ningbo Medical Center Lihuili Hospital, Ningbo 315040, China. FAU - Qiu, Shijie AU - Qiu S AD - Department of Otorhinolaryngology, Ningbo Medical Center Lihuili Hospital, Ningbo 315040, China. FAU - Li, Qun AU - Li Q AD - Department of Otorhinolaryngology, Ningbo Medical Center Lihuili Hospital, Ningbo 315040, China. FAU - Cui, Xiang AU - Cui X AD - Ningbo University School of Medicine, Ningbo 315040, China. LA - chi PT - Journal Article PL - China TA - Zhejiang Da Xue Xue Bao Yi Xue Ban JT - Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences JID - 100927946 RN - 0 (Antineoplastic Agents) RN - 0 (Inhibitor of Apoptosis Proteins) RN - 0 (Ki-67 Antigen) RN - 0 (RNA, Messenger) RN - 0 (RNA, Small Interfering) RN - EC 2.7.1.- (Phosphatidylinositol 3-Kinases) RN - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) RN - EC 3.1.2.- (Protein Deglycase DJ-1) RN - EC 3.1.3.67 (PTEN Phosphohydrolase) RN - EC 3.4.22.- (Caspase 3) SB - IM MH - Animals MH - Antineoplastic Agents/pharmacology MH - Apoptosis/drug effects/genetics MH - Carcinoma, Squamous Cell/*chemistry/genetics/*physiopathology MH - Caspase 3/analysis/drug effects MH - Cell Line, Tumor/*chemistry/*drug effects/physiology/transplantation MH - Cell Proliferation/drug effects MH - Down-Regulation MH - Gene Expression Regulation/*drug effects/genetics/physiology MH - Head and Neck Neoplasms/*chemistry/genetics/*physiopathology MH - Heterografts/drug effects/physiology MH - Humans MH - Inhibitor of Apoptosis Proteins/analysis/drug effects MH - Ki-67 Antigen/analysis/drug effects MH - Laryngeal Neoplasms/*chemistry/genetics/*physiopathology MH - Mice, Nude MH - PTEN Phosphohydrolase/analysis/drug effects MH - Phosphatidylinositol 3-Kinases/drug effects MH - Protein Deglycase DJ-1/*pharmacology MH - Proto-Oncogene Proteins c-akt/drug effects MH - RNA Interference/*physiology MH - RNA, Messenger/*pharmacology MH - RNA, Small Interfering/*physiology MH - Signal Transduction/drug effects/genetics/physiology MH - Squamous Cell Carcinoma of Head and Neck PMC - PMC10396977 EDAT- 2016/11/22 06:00 MHDA- 2017/09/26 06:00 PMCR- 2016/07/25 CRDT- 2016/11/22 06:00 PHST- 2016/11/22 06:00 [entrez] PHST- 2016/11/22 06:00 [pubmed] PHST- 2017/09/26 06:00 [medline] PHST- 2016/07/25 00:00 [pmc-release] AID - zjdxxbyxb-45-4-349 [pii] AID - 10.3785/j.issn.1008-9292.2016.07.04 [doi] PST - ppublish SO - Zhejiang Da Xue Xue Bao Yi Xue Ban. 2016 May 25;45(4):349-355. doi: 10.3785/j.issn.1008-9292.2016.07.04.