PMID- 27870827
OWN - NLM
STAT- MEDLINE
DCOM- 20171121
LR  - 20181113
IS  - 1530-0447 (Electronic)
IS  - 0031-3998 (Print)
IS  - 0031-3998 (Linking)
VI  - 81
IP  - 4
DP  - 2017 Apr
TI  - Augmented Th17-type immune responses in preterm neonates exposed to histologic 
      chorioamnionitis.
PG  - 639-645
LID - 10.1038/pr.2016.254 [doi]
AB  - BACKGROUND: Histologic chorioamnionitis (HCA) is a placental inflammatory 
      disorder that frequently precedes preterm delivery. HCA increases risk for 
      long-standing inflammatory injury and may influence immune programming, 
      particularly in preterm (PT) neonates. We hypothesized that HCA exposure is 
      associated with an increased circulating frequency of proinflammatory, Th17-type 
      responses. METHODS: Placental cord blood was collected from HCA-exposed or 
      control neonates (23-41 wk gestation). Frequencies of Th17 and T regulatory 
      (Treg) cells and assessments of Th17-type features in CD4 and Treg cells were 
      determined by flow cytometric analysis. RESULTS: Cord blood samples from 31 PT 
      and 17 term neonates were analyzed by flow cytometry. A diagnosis of HCA in 
      extremely PT (EPT, GA </= 30 wk) gestations was associated with the highest cord 
      blood frequencies of progenitor (pTh17, CD4(+)CD161(+)) and mature (mTh17, 
      CD4(+)CD161(+)CCR6(+)) Th17 cells. Preterm neonates exposed to HCA also exhibited 
      elevated cord blood frequencies of IL-17(+) Treg cells, as well as T cells with 
      effector memory phenotype (TEM) that coexpressed Th17-type surface antigens. 
      CONCLUSION: Th17-type responses are amplified in preterm neonates exposed to HCA. 
      We speculate that a Th17 bias may potentiate the inflammatory responses and 
      related morbidity observed in preterm neonates whose immune systems have been 
      "primed" by HCA exposure.
FAU - Rito, Daniel C
AU  - Rito DC
AD  - Department of Neonatology, Henry Ford Medical Group, Detroit, Michigan.
FAU - Viehl, Luke T
AU  - Viehl LT
AD  - Department of Pediatrics, Saint Louis University, St. Louis, Missouri.
FAU - Buchanan, Paula M
AU  - Buchanan PM
AD  - Department of Pediatrics, Saint Louis University, St. Louis, Missouri.
AD  - School of Public Health & Social Justice, Saint Louis University, St. Louis, 
      Missouri.
FAU - Haridas, Seema
AU  - Haridas S
AD  - Department of Pediatrics, Saint Louis University, St. Louis, Missouri.
FAU - Koenig, Joyce M
AU  - Koenig JM
AD  - Department of Pediatrics, Saint Louis University, St. Louis, Missouri.
AD  - Department of Molecular Microbiology & Immunology, Saint Louis University, St. 
      Louis, Missouri.
LA  - eng
GR  - R21 AI094478/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Observational Study
DEP - 20161121
PL  - United States
TA  - Pediatr Res
JT  - Pediatric research
JID - 0100714
RN  - 0 (IL17A protein, human)
RN  - 0 (Interleukin-17)
SB  - IM
MH  - Adult
MH  - CD4-Positive T-Lymphocytes/cytology
MH  - Case-Control Studies
MH  - Chorioamnionitis/*blood/*immunology
MH  - Female
MH  - Fetal Blood/metabolism
MH  - Flow Cytometry
MH  - Humans
MH  - Immune System
MH  - Infant, Newborn
MH  - Infant, Premature
MH  - Inflammation
MH  - Interleukin-17/immunology
MH  - Phenotype
MH  - Placenta/metabolism
MH  - Pregnancy
MH  - Prospective Studies
MH  - T-Lymphocytes, Regulatory/cytology
MH  - Th17 Cells/*cytology
MH  - Young Adult
PMC - PMC5395318
MID - NIHMS830574
EDAT- 2016/11/22 06:00
MHDA- 2017/11/29 06:00
PMCR- 2017/05/21
CRDT- 2016/11/22 06:00
PHST- 2016/06/01 00:00 [received]
PHST- 2016/10/09 00:00 [accepted]
PHST- 2016/11/22 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2016/11/22 06:00 [entrez]
PHST- 2017/05/21 00:00 [pmc-release]
AID - pr2016254 [pii]
AID - 10.1038/pr.2016.254 [doi]
PST - ppublish
SO  - Pediatr Res. 2017 Apr;81(4):639-645. doi: 10.1038/pr.2016.254. Epub 2016 Nov 21.