PMID- 27872161
OWN - NLM
STAT- MEDLINE
DCOM- 20170907
LR  - 20220409
IS  - 1469-9001 (Electronic)
IS  - 1355-8382 (Print)
IS  - 1355-8382 (Linking)
VI  - 23
IP  - 2
DP  - 2017 Feb
TI  - Identification of urinary exosomal noncoding RNAs as novel biomarkers in chronic 
      kidney disease.
PG  - 142-152
LID - 10.1261/rna.058834.116 [doi]
AB  - In chronic kidney disease (CKD), the decline in the glomerular filtration rate is 
      associated with increased morbidity and mortality and thus poses a major 
      challenge for healthcare systems. While the contribution of tissue-derived miRNAs 
      and mRNAs to CKD progression has been extensively studied, little is known about 
      the role of urinary exosomes and their association with CKD. Exosomes are small, 
      membrane-derived endocytic vesicles that contribute to cell-to-cell communication 
      and are present in various body fluids, such as blood or urine. Next-generation 
      sequencing approaches have revealed that exosomes are enriched in noncoding RNAs 
      and thus exhibit great potential for sensitive nucleic acid biomarkers in various 
      human diseases. Therefore, in this study we aimed to identify urinary exosomal 
      ncRNAs as novel biomarkers for diagnosis of CKD. Since up to now most approaches 
      have focused on the class of miRNAs, we extended our analysis to several other 
      noncoding RNA classes, such as tRNAs, tRNA fragments (tRFs), mitochondrial tRNAs, 
      or lincRNAs. For their computational identification from RNA-seq data, we 
      developed a novel computational pipeline, designated as ncRNASeqScan. By these 
      analyses, in CKD patients we identified 30 differentially expressed ncRNAs, 
      derived from urinary exosomes, as suitable biomarkers for early diagnosis. 
      Thereby, miRNA-181a appeared as the most robust and stable potential biomarker, 
      being significantly decreased by about 200-fold in exosomes of CKD patients 
      compared to healthy controls. Using a cell culture system for CKD indicated that 
      urinary exosomes might indeed originate from renal proximal tubular epithelial 
      cells.
CI  - (c) 2017 Khurana et al.; Published by Cold Spring Harbor Laboratory Press for the 
      RNA Society.
FAU - Khurana, Rimpi
AU  - Khurana R
AD  - Division of Genomics and RNomics, Biocenter, Medical University Innsbruck, 6020 
      Innsbruck, Austria.
FAU - Ranches, Glory
AU  - Ranches G
AD  - Division of Genomics and RNomics, Biocenter, Medical University Innsbruck, 6020 
      Innsbruck, Austria.
FAU - Schafferer, Simon
AU  - Schafferer S
AD  - Division of Genomics and RNomics, Biocenter, Medical University Innsbruck, 6020 
      Innsbruck, Austria.
FAU - Lukasser, Melanie
AU  - Lukasser M
AD  - Division of Genomics and RNomics, Biocenter, Medical University Innsbruck, 6020 
      Innsbruck, Austria.
FAU - Rudnicki, Michael
AU  - Rudnicki M
AUID- ORCID: 0000-0002-7896-2600
AD  - Department of Internal Medicine IV, Nephrology and Hypertension, Medical 
      University Innsbruck, 6020 Innsbruck, Austria.
FAU - Mayer, Gert
AU  - Mayer G
AD  - Department of Internal Medicine IV, Nephrology and Hypertension, Medical 
      University Innsbruck, 6020 Innsbruck, Austria.
FAU - Huttenhofer, Alexander
AU  - Huttenhofer A
AD  - Division of Genomics and RNomics, Biocenter, Medical University Innsbruck, 6020 
      Innsbruck, Austria.
AD  - i-med GenomeSeq Core, 6020 Innsbruck, Austria.
LA  - eng
PT  - Journal Article
DEP - 20161121
PL  - United States
TA  - RNA
JT  - RNA (New York, N.Y.)
JID - 9509184
RN  - 0 (Biomarkers)
RN  - 0 (MIrn181 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Mitochondrial)
RN  - 63231-63-0 (RNA)
RN  - 9014-25-9 (RNA, Transfer)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers/urine
MH  - Case-Control Studies
MH  - Early Diagnosis
MH  - Epithelial Cells/*metabolism/pathology
MH  - Exosomes/*chemistry/metabolism
MH  - Female
MH  - Glomerular Filtration Rate
MH  - High-Throughput Nucleotide Sequencing
MH  - Humans
MH  - Kidney Tubules, Proximal/*metabolism/pathology
MH  - Male
MH  - MicroRNAs/*urine
MH  - Middle Aged
MH  - Molecular Sequence Annotation
MH  - RNA/urine
MH  - RNA, Long Noncoding/urine
MH  - RNA, Mitochondrial
MH  - RNA, Transfer/urine
MH  - Renal Insufficiency, Chronic/*diagnosis/pathology/urine
MH  - Sequence Analysis, RNA
MH  - Severity of Illness Index
PMC - PMC5238789
OTO - NOTNLM
OT  - chronic kidney disease
OT  - lincRNAs
OT  - miRNAs
OT  - ncRNAs
OT  - sequencing
OT  - tRFs
OT  - tRNAs
OT  - urinary exosomes
EDAT- 2016/11/23 06:00
MHDA- 2017/09/08 06:00
PMCR- 2017/02/01
CRDT- 2016/11/23 06:00
PHST- 2016/08/23 00:00 [received]
PHST- 2016/11/08 00:00 [accepted]
PHST- 2016/11/23 06:00 [pubmed]
PHST- 2017/09/08 06:00 [medline]
PHST- 2016/11/23 06:00 [entrez]
PHST- 2017/02/01 00:00 [pmc-release]
AID - rna.058834.116 [pii]
AID - RA [pii]
AID - 10.1261/rna.058834.116 [doi]
PST - ppublish
SO  - RNA. 2017 Feb;23(2):142-152. doi: 10.1261/rna.058834.116. Epub 2016 Nov 21.