PMID- 27873195
OWN - NLM
STAT- MEDLINE
DCOM- 20170329
LR  - 20221207
IS  - 1573-0972 (Electronic)
IS  - 0959-3993 (Linking)
VI  - 33
IP  - 1
DP  - 2017 Jan
TI  - Community structure of a sulfate-reducing consortium in lead-contaminated 
      wastewater treatment process.
PG  - 10
AB  - This study evaluated the capacity to remove lead by an indigenous consortium of 
      five sulfate-reducing bacteria (SRB): Desulfobacterium autotrophicum, 
      Desulfomicrobium salsugmis, Desulfomicrobium escambiense, Desulfovibrio vulgaris, 
      and Desulfovibrio carbinolicus, using continuous moving bed biofilm reactor 
      systems. Four continuous moving bed biofilm reactors (referred as R1-R4) were run 
      in parallel for 40 days at lead loading rates of 0, 20, 30 and 40 mg l(-1) 
      day(-1), respectively. The impact of lead on community structure of the SRB 
      consortium was investigated by dsrB gene-based denaturing gradient gel 
      electrophoresis (dsrB-based DGGE), fluorescence in situ hybridization (FISH) and 
      chemical analysis. These results indicated that D. escambiense and D. 
      carbinolicus were dominant in all analyzed samples and played a key role in lead 
      removal in R2 (20 mg l(-1) day(-1)) and R3 (30 mg l(-1) day(-)(1)). However, in 
      R4 (40 mg l(-1) day(-1)), these two strains were barely detected by FISH and 
      dsrB-based DGGE. As a result, SRB activity was severely affected by lead 
      toxicity. High lead removal efficiencies of lead (99-100%) were observed in R2 
      and R3 throughout the operation, whereas that in R4 was significantly decreased 
      (91%) after 40 days of operation. This data strongly implied that the 
      investigated SRB consortium might have potential application for lead removal. 
      Moreover, to improve the efficiency of the lead treatment process, the lead 
      loading rates below the inhibitory level to SRB activity should be selected.
FAU - Nguyen, Yen T
AU  - Nguyen YT
AD  - Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang 
      Quoc Viet, Cau Giay, Hanoi, Vietnam.
FAU - Kieu, Hoa T Q
AU  - Kieu HT
AD  - Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang 
      Quoc Viet, Cau Giay, Hanoi, Vietnam. ktquynhhoa@ibt.ac.vn.
FAU - West, Stephanie
AU  - West S
AD  - Engler-Bunte-Institut, Chair of Water Chemistry and Water Technology, Karlsruhe 
      Institute of Technology, Engler-Bunte-Ring 9, 76131, Karlsruhe, Germany.
FAU - Dang, Yen T
AU  - Dang YT
AD  - Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang 
      Quoc Viet, Cau Giay, Hanoi, Vietnam.
FAU - Horn, Harald
AU  - Horn H
AD  - Engler-Bunte-Institut, Chair of Water Chemistry and Water Technology, Karlsruhe 
      Institute of Technology, Engler-Bunte-Ring 9, 76131, Karlsruhe, Germany.
LA  - eng
PT  - Journal Article
DEP - 20161121
PL  - Germany
TA  - World J Microbiol Biotechnol
JT  - World journal of microbiology & biotechnology
JID - 9012472
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Waste Water)
RN  - 0 (Water Pollutants, Chemical)
RN  - 2P299V784P (Lead)
SB  - IM
MH  - Biodegradation, Environmental
MH  - Bioreactors/microbiology
MH  - DNA, Bacterial/genetics
MH  - Denaturing Gradient Gel Electrophoresis
MH  - In Situ Hybridization, Fluorescence
MH  - Lead/*metabolism
MH  - Oxidation-Reduction
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sulfur-Reducing Bacteria/*classification/genetics/*isolation & purification
MH  - Wastewater/*microbiology
MH  - Water Pollutants, Chemical/*metabolism
OTO - NOTNLM
OT  - Denaturing gradient gel electrophoresis
OT  - Fluorescent in situ hybridization
OT  - Lead removal
OT  - Sulfate-reducing bacteria
OT  - dsrB gene
EDAT- 2016/11/23 06:00
MHDA- 2017/03/31 06:00
CRDT- 2016/11/23 06:00
PHST- 2016/05/24 00:00 [received]
PHST- 2016/11/14 00:00 [accepted]
PHST- 2016/11/23 06:00 [entrez]
PHST- 2016/11/23 06:00 [pubmed]
PHST- 2017/03/31 06:00 [medline]
AID - 10.1007/s11274-016-2180-7 [pii]
AID - 10.1007/s11274-016-2180-7 [doi]
PST - ppublish
SO  - World J Microbiol Biotechnol. 2017 Jan;33(1):10. doi: 10.1007/s11274-016-2180-7. 
      Epub 2016 Nov 21.