PMID- 27873367
OWN - NLM
STAT- MEDLINE
DCOM- 20180206
LR  - 20180619
IS  - 1460-9568 (Electronic)
IS  - 0953-816X (Linking)
VI  - 45
IP  - 2
DP  - 2017 Jan
TI  - miR-219 attenuates demyelination in cuprizone-induced demyelinated mice by 
      regulating monocarboxylate transporter 1.
PG  - 249-259
LID - 10.1111/ejn.13485 [doi]
AB  - Remyelination is limited in patients with multiple sclerosis (MS) due to the 
      difficulties in recruiting proliferating oligodendrocyte precursors (OPCs), the 
      inhibition of OPC differentiation and/or maturation, and/or failure in the 
      generation of the myelin sheath. In vitro studies have revealed that miR-219 is 
      necessary for OPC differentiation and monocarboxylate transporter 1 (MCT1) plays 
      a vital role in oligodendrocyte maturation and myelin synthesis. Herein, we 
      hypothesized that miR-219 might promote oligodendrocyte differentiation and 
      attenuate demyelination in a cuprizone (CPZ)-induced demyelinated model by 
      regulating the expression of MCT1. We found that CPZ-treated mice exhibited 
      significantly increased anxiety in the open field test. However, miR-219 reduced 
      anxiety as shown by an increase in the total distance, the central distance and 
      the mean amount of time spent in the central area. miR-219 decreased the quantity 
      of OPCs and increased the number of oligodendrocytes and the level of myelin 
      basic protein (MBP) and cyclic nucleotide 3' phosphodiesterase (CNP) protein. 
      Ultrastructural studies further confirmed that the extent of demyelination was 
      attenuated by miR-219 overexpression. Meanwhile, miR-219 also greatly enhanced 
      MCT1 expression via suppression of oligodendrocyte differentiation inhibitors, 
      Sox6 and Hes5, treatment with the MCT1 inhibitor alpha-cyano-4-hydroxycinnamate 
      (4-CIN) reduced the number of oligodendrocytes and the protein levels of MBP and 
      CNP. Taken together, these results suggest a novel mode of action of miR-219 via 
      MCT1 in vivo and may provide a new potential remyelination therapeutic target.
CI  - (c) 2016 Federation of European Neuroscience Societies and John Wiley & Sons Ltd.
FAU - Liu, Sihan
AU  - Liu S
AD  - Research Center for Neurobiology, Jiangsu Province Key Laboratory of 
      Anesthesiology, Xuzhou Medical University, 209 Tongshan Road, Xuzhou, 221000, 
      China.
AD  - Department of Neurology, Affiliated Hospital of Xuzhou Medical University, 99 
      Huaihai Road, Xuzhou, 221009, China.
FAU - Ren, Chuanlu
AU  - Ren C
AD  - Department of Laboratory, No. 100 Hospital of CPLA, Suzhou, China.
FAU - Qu, Xuebin
AU  - Qu X
AD  - Research Center for Neurobiology, Jiangsu Province Key Laboratory of 
      Anesthesiology, Xuzhou Medical University, 209 Tongshan Road, Xuzhou, 221000, 
      China.
FAU - Wu, Xiuxiang
AU  - Wu X
AD  - Research Center for Neurobiology, Jiangsu Province Key Laboratory of 
      Anesthesiology, Xuzhou Medical University, 209 Tongshan Road, Xuzhou, 221000, 
      China.
FAU - Dong, Fuxing
AU  - Dong F
AD  - Research Center for Neurobiology, Jiangsu Province Key Laboratory of 
      Anesthesiology, Xuzhou Medical University, 209 Tongshan Road, Xuzhou, 221000, 
      China.
FAU - Chand, Yadav Kaushal
AU  - Chand YK
AD  - Research Center for Neurobiology, Jiangsu Province Key Laboratory of 
      Anesthesiology, Xuzhou Medical University, 209 Tongshan Road, Xuzhou, 221000, 
      China.
FAU - Fan, Hongbin
AU  - Fan H
AD  - Department of Neurology, Affiliated Hospital of Xuzhou Medical University, 99 
      Huaihai Road, Xuzhou, 221009, China.
FAU - Yao, Ruiqin
AU  - Yao R
AUID- ORCID: 0000-0001-7617-5438
AD  - Research Center for Neurobiology, Jiangsu Province Key Laboratory of 
      Anesthesiology, Xuzhou Medical University, 209 Tongshan Road, Xuzhou, 221000, 
      China.
FAU - Geng, Deqin
AU  - Geng D
AD  - Department of Neurology, Affiliated Hospital of Xuzhou Medical University, 99 
      Huaihai Road, Xuzhou, 221009, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Coumaric Acids)
RN  - 0 (MIRN219 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (Monocarboxylic Acid Transporters)
RN  - 0 (Symporters)
RN  - 0 (monocarboxylate transport protein 1)
RN  - 28166-41-8 (alpha-cyano-4-hydroxycinnamate)
RN  - 5N16U7E0AO (Cuprizone)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/drug effects/physiology
MH  - Cell Proliferation/physiology
MH  - Cells, Cultured
MH  - Corpus Callosum/metabolism
MH  - Coumaric Acids/*pharmacology
MH  - Cuprizone/*pharmacology
MH  - Demyelinating Diseases/*drug therapy/genetics
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*genetics
MH  - Monocarboxylic Acid Transporters/*metabolism
MH  - Myelin Sheath/drug effects/metabolism
MH  - Oligodendroglia/*drug effects/metabolism
MH  - Stem Cells/classification/metabolism
MH  - Symporters/*metabolism
OTO - NOTNLM
OT  - demyelination
OT  - microRNA
OT  - monocarboxylate transporter 1
OT  - oligodendrocyte
OT  - remyelination
EDAT- 2016/11/23 06:00
MHDA- 2018/02/07 06:00
CRDT- 2016/11/23 06:00
PHST- 2016/09/11 00:00 [received]
PHST- 2016/11/17 00:00 [revised]
PHST- 2016/11/18 00:00 [accepted]
PHST- 2016/11/23 06:00 [pubmed]
PHST- 2018/02/07 06:00 [medline]
PHST- 2016/11/23 06:00 [entrez]
AID - 10.1111/ejn.13485 [doi]
PST - ppublish
SO  - Eur J Neurosci. 2017 Jan;45(2):249-259. doi: 10.1111/ejn.13485.