PMID- 27874239
OWN - NLM
STAT- MEDLINE
DCOM- 20180829
LR  - 20190110
IS  - 1521-3773 (Electronic)
IS  - 1433-7851 (Linking)
VI  - 55
IP  - 50
DP  - 2016 Dec 12
TI  - Biocatalytic Properties and Structural Analysis of Phloroglucinol Reductases.
PG  - 15531-15534
LID - 10.1002/anie.201607494 [doi]
AB  - Phloroglucinol reductases (PGRs) are involved in anaerobic degradation in 
      bacteria, in which they catalyze the dearomatization of phloroglucinol into 
      dihydrophloroglucinol. We identified three PGRs, from different bacterial 
      species, that are members of the family of NAD(P)H-dependent short-chain 
      dehydrogenases/reductases (SDRs). In addition to catalyzing the reduction of the 
      physiological substrate, the three enzymes exhibit activity towards 
      2,4,6-trihydroxybenzaldehyde, 2,4,6-trihydroxyacetophenone, and methyl 
      2,4,6-trihydroxybenzoate. Structural elucidation of PGRcl and comparison to known 
      SDRs revealed a high degree of conservation. Several amino acid positions were 
      identified as being conserved within the PGR subfamily and might be involved in 
      substrate differentiation. The results enable the enzymatic dearomatization of 
      monoaromatic phenol derivatives and provide insight into the functional diversity 
      that may be found in families of enzymes displaying a high degree of structural 
      homology.
CI  - (c) 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Conradt, David
AU  - Conradt D
AUID- ORCID: 0000-0001-8984-7694
AD  - Institut fur Pharmazeutische Wissenschaften, Albert-Ludwigs-Universitat Freiburg, 
      Albertstrasse 25, 79104, Freiburg, Germany.
FAU - Hermann, Bianca
AU  - Hermann B
AD  - Institut fur Biochemie, Albert-Ludwigs-Universitat Freiburg, Albertstrasse 21, 
      79104, Freiburg, Germany.
FAU - Gerhardt, Stefan
AU  - Gerhardt S
AUID- ORCID: 0000-0002-9098-8475
AD  - Institut fur Biochemie, Albert-Ludwigs-Universitat Freiburg, Albertstrasse 21, 
      79104, Freiburg, Germany.
FAU - Einsle, Oliver
AU  - Einsle O
AD  - Institut fur Biochemie, Albert-Ludwigs-Universitat Freiburg, Albertstrasse 21, 
      79104, Freiburg, Germany.
FAU - Muller, Michael
AU  - Muller M
AUID- ORCID: 0000-0002-2742-4992
AD  - Institut fur Pharmazeutische Wissenschaften, Albert-Ludwigs-Universitat Freiburg, 
      Albertstrasse 25, 79104, Freiburg, Germany.
LA  - eng
PT  - Journal Article
DEP - 20161122
PL  - Germany
TA  - Angew Chem Int Ed Engl
JT  - Angewandte Chemie (International ed. in English)
JID - 0370543
RN  - 0 (Acetophenones)
RN  - 0 (Benzaldehydes)
RN  - 623D3XG80C (methyl gallate)
RN  - 632XD903SP (Gallic Acid)
RN  - 8L7XD8830T (2,4,6-trihydroxyacetophenone)
RN  - EC 1.3.- (Oxidoreductases Acting on CH-CH Group Donors)
RN  - EC 1.3.1.57 (phloroglucinol reductase)
RN  - I70C45KRO9 (2,4,6-trihydroxybenzaldehyde)
SB  - IM
MH  - Acetophenones/metabolism
MH  - Bacteria/chemistry/*enzymology/metabolism
MH  - Benzaldehydes/metabolism
MH  - Biocatalysis
MH  - Gallic Acid/analogs & derivatives/metabolism
MH  - Oxidoreductases Acting on CH-CH Group Donors/*chemistry/*metabolism
MH  - Protein Conformation
MH  - Substrate Specificity
OTO - NOTNLM
OT  - dearomatization
OT  - enzyme catalysis
OT  - flavonoids
OT  - redox enzymes
OT  - reductases
EDAT- 2016/11/23 06:00
MHDA- 2018/08/30 06:00
CRDT- 2016/11/23 06:00
PHST- 2016/08/02 00:00 [received]
PHST- 2016/09/09 00:00 [revised]
PHST- 2016/11/23 06:00 [pubmed]
PHST- 2018/08/30 06:00 [medline]
PHST- 2016/11/23 06:00 [entrez]
AID - 10.1002/anie.201607494 [doi]
PST - ppublish
SO  - Angew Chem Int Ed Engl. 2016 Dec 12;55(50):15531-15534. doi: 
      10.1002/anie.201607494. Epub 2016 Nov 22.