PMID- 27875274
OWN - NLM
STAT- MEDLINE
DCOM- 20170803
LR  - 20201209
IS  - 1477-9137 (Electronic)
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 129
IP  - 24
DP  - 2016 Dec 15
TI  - SIRT1 inhibits EV71 genome replication and RNA translation by interfering with 
      the viral polymerase and 5'UTR RNA.
PG  - 4534-4547
AB  - Enterovirus 71 (EV71) possesses a single-stranded positive RNA genome that 
      contains a single open reading frame (ORF) flanked by a 5' untranslated region 
      (5'UTR) and a polyadenylated 3'UTR. Here, we demonstrated that EV71 activates the 
      production of silent mating type information regulation 2 homolog 1 (SIRT1), a 
      histone deacetylase (HDAC). EV71 further stimulates SIRT1 sumoylation and 
      deacetylase activity, and enhances SIRT1 translocation from the nucleus to the 
      cytoplasm. More interestingly, activated SIRT1 subsequently binds with the EV71 
      3D(pol) protein (a viral RNA-dependent RNA polymerase, RdRp) to repress the 
      acetylation and RdRp activity of 3D(pol), resulting in the attenuation of viral 
      genome replication. Moreover, SIRT1 interacts with the cloverleaf structure of 
      the EV71 RNA 5'UTR to inhibit viral RNA transcription, and binds to the internal 
      ribosome entry site (IRES) of the EV71 5'UTR to attenuate viral RNA translation. 
      Thus, EV71 stimulates SIRT1 production and activity, which in turn represses EV71 
      genome replication by inhibiting viral polymerase, and attenuates EV71 RNA 
      transcription and translation by interfering with viral RNA. These results 
      uncover a new function of SIRT1 and reveal a new mechanism underlying the 
      regulation of EV71 replication.
CI  - (c) 2016. Published by The Company of Biologists Ltd.
FAU - Han, Yang
AU  - Han Y
AD  - State Key Laboratory of Virology and College of Life Sciences, Wuhan University, 
      Wuhan 430072, China.
FAU - Wang, Lvyin
AU  - Wang L
AD  - State Key Laboratory of Virology and College of Life Sciences, Wuhan University, 
      Wuhan 430072, China.
FAU - Cui, Jin
AU  - Cui J
AD  - State Key Laboratory of Virology and College of Life Sciences, Wuhan University, 
      Wuhan 430072, China.
FAU - Song, Yu
AU  - Song Y
AD  - State Key Laboratory of Virology and College of Life Sciences, Wuhan University, 
      Wuhan 430072, China.
FAU - Luo, Zhen
AU  - Luo Z
AD  - State Key Laboratory of Virology and College of Life Sciences, Wuhan University, 
      Wuhan 430072, China.
FAU - Chen, Junbo
AU  - Chen J
AD  - State Key Laboratory of Virology and College of Life Sciences, Wuhan University, 
      Wuhan 430072, China.
FAU - Xiong, Ying
AU  - Xiong Y
AD  - State Key Laboratory of Virology and College of Life Sciences, Wuhan University, 
      Wuhan 430072, China.
FAU - Zhang, Qi
AU  - Zhang Q
AD  - State Key Laboratory of Virology and College of Life Sciences, Wuhan University, 
      Wuhan 430072, China.
FAU - Liu, Fang
AU  - Liu F
AD  - State Key Laboratory of Virology and College of Life Sciences, Wuhan University, 
      Wuhan 430072, China.
FAU - Ho, Wenzhe
AU  - Ho W
AD  - State Key Laboratory of Virology and College of Life Sciences, Wuhan University, 
      Wuhan 430072, China.
FAU - Liu, Yingle
AU  - Liu Y
AD  - State Key Laboratory of Virology and College of Life Sciences, Wuhan University, 
      Wuhan 430072, China jwu@whu.edu.cn mvlwu@whu.edu.cn wukailang@whu.edu.cn.
FAU - Wu, Kailang
AU  - Wu K
AUID- ORCID: 0000-0002-8326-2895
AD  - State Key Laboratory of Virology and College of Life Sciences, Wuhan University, 
      Wuhan 430072, China jwu@whu.edu.cn mvlwu@whu.edu.cn wukailang@whu.edu.cn.
FAU - Wu, Jianguo
AU  - Wu J
AD  - State Key Laboratory of Virology and College of Life Sciences, Wuhan University, 
      Wuhan 430072, China jwu@whu.edu.cn mvlwu@whu.edu.cn wukailang@whu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20161114
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (3' Untranslated Regions)
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Internal Ribosome Entry Sites)
RN  - 0 (RNA, Viral)
RN  - 0 (Small Ubiquitin-Related Modifier Proteins)
RN  - EC 2.7.7.48 (RNA-Dependent RNA Polymerase)
RN  - EC 3.5.1.- (Sirtuin 1)
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - 5' Untranslated Regions/*genetics
MH  - Acetylation
MH  - Cell Line
MH  - Cell Nucleus
MH  - Enterovirus A, Human/*genetics/physiology
MH  - *Genome, Viral
MH  - Humans
MH  - Internal Ribosome Entry Sites
MH  - Models, Biological
MH  - Nucleic Acid Conformation
MH  - Protein Binding
MH  - *Protein Biosynthesis
MH  - RNA, Viral/*metabolism
MH  - RNA-Dependent RNA Polymerase/*metabolism
MH  - Sirtuin 1/*metabolism
MH  - Small Ubiquitin-Related Modifier Proteins/metabolism
MH  - Sumoylation
MH  - *Virus Replication
PMC - PMC5201017
OTO - NOTNLM
OT  - Enterovirus 71
OT  - HDAC
OT  - IRES
OT  - Replication
OT  - SIRT1
OT  - Transcription
OT  - Translation
OT  - Viral infection
COIS- The authors declare no competing or financial interests.
EDAT- 2016/11/23 06:00
MHDA- 2017/08/05 06:00
PMCR- 2016/12/15
CRDT- 2016/11/23 06:00
PHST- 2016/06/17 00:00 [received]
PHST- 2016/11/02 00:00 [accepted]
PHST- 2016/11/23 06:00 [pubmed]
PHST- 2017/08/05 06:00 [medline]
PHST- 2016/11/23 06:00 [entrez]
PHST- 2016/12/15 00:00 [pmc-release]
AID - jcs.193698 [pii]
AID - JCS193698 [pii]
AID - 10.1242/jcs.193698 [doi]
PST - ppublish
SO  - J Cell Sci. 2016 Dec 15;129(24):4534-4547. doi: 10.1242/jcs.193698. Epub 2016 Nov 
      14.