PMID- 27876202
OWN - NLM
STAT- MEDLINE
DCOM- 20171215
LR  - 20181202
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Linking)
VI  - 35
IP  - 1
DP  - 2017 Jan 3
TI  - An interferon inducing porcine reproductive and respiratory syndrome virus 
      vaccine candidate elicits protection against challenge with the heterologous 
      virulent type 2 strain VR-2385 in pigs.
PG  - 125-131
LID - S0264-410X(16)31067-2 [pii]
LID - 10.1016/j.vaccine.2016.11.020 [doi]
AB  - Achieving consistent protection by vaccinating pigs against porcine reproductive 
      and respiratory syndrome virus (PRRSV) remains difficult. Recently, an 
      interferon-inducing PRRSV vaccine candidate strain A2MC2 was demonstrated to be 
      attenuated and induced neutralizing antibodies. The objective of this study was 
      to determine the efficacy of passage 90 of A2MC2 (A2P90) to protect pigs against 
      challenge with moderately virulent PRRSV strain VR-2385 (92.3% nucleic acid 
      identity with A2MC2) and highly virulent atypical PRRSV MN184 (84.5% nucleic acid 
      identity with A2MC2). Forty 3-week old pigs were randomly assigned to five groups 
      including a NEG-CONTROL group (non-vaccinated, non-challenged), VAC-VR2385 
      (vaccinated, challenged with strain VR-2385), VR2385 (challenged with strain 
      VR-2385), VAC-MN184 (vaccinated, challenged with strain MN184) and a MN184 group 
      (challenged with MN184 virus). Vaccination was done at 3weeks of age followed by 
      challenge at 8weeks of age. No viremia was detectable in any of the vaccinated 
      pigs; however, by the time of challenge, 15/16 vaccinated pigs had seroconverted 
      based on ELISA and had neutralizing antibodies against a homologous strain with 
      titers ranging from 8 to 128. Infection with VR-2385 resulted in mild-to-moderate 
      clinical disease and lesions. For VR-2385 infected pigs, vaccination 
      significantly lowered PRRSV viremia and nasal shedding by 9days post challenge 
      (dpc), significantly reduced macroscopic lung lesions, and significantly 
      increased the average daily weight gain compared to the non-vaccinated pigs. 
      Infection with MN184 resulted in moderate-to-severe clinical disease and lesions 
      regardless of vaccination status; however, vaccinated pigs had significantly less 
      nasal shedding by dpc 5 compared to non-vaccinated pigs. Under the study 
      conditions, the A2P90 vaccine strain was attenuated without detectable shedding, 
      improved weight gain, and offered protection to the pigs challenged with VR-2385 
      by reduction of virus load and macroscopic lung lesions. Further work is needed 
      to investigate different vaccination and challenge protocols, including routes, 
      doses, timing and strains.
CI  - Copyright A(c) 2016 Elsevier Ltd. All rights reserved.
FAU - Fontanella, Eve
AU  - Fontanella E
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, USA.
FAU - Ma, Zexu
AU  - Ma Z
AD  - Molecular Virology Laboratory, VA-MD College of Veterinary Medicine and Maryland 
      Pathogen Research Institute, University of Maryland, College Park, MD, USA.
FAU - Zhang, Yanjin
AU  - Zhang Y
AD  - Molecular Virology Laboratory, VA-MD College of Veterinary Medicine and Maryland 
      Pathogen Research Institute, University of Maryland, College Park, MD, USA.
FAU - de Castro, Alessandra M M G
AU  - de Castro AM
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, USA.
FAU - Shen, Huigang
AU  - Shen H
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, USA.
FAU - Halbur, Patrick G
AU  - Halbur PG
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, USA.
FAU - Opriessnig, Tanja
AU  - Opriessnig T
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, USA; The Roslin Institute 
      and The Royal (Dick) School of Veterinary Studies, University of Edinburgh, 
      Midlothian, UK. Electronic address: Tanja.Opriessnig@roslin.ed.ac.uk.
LA  - eng
GR  - Biotechnology and Biological Sciences Research Council/United Kingdom
PT  - Journal Article
DEP - 20161118
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Viral Vaccines)
RN  - 9008-11-1 (Interferons)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/blood
MH  - Antibodies, Viral/blood
MH  - Antibody Formation
MH  - Body Weight
MH  - Interferons/*metabolism
MH  - Lung/pathology
MH  - Porcine Reproductive and Respiratory Syndrome/*prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Random Allocation
MH  - Swine
MH  - Treatment Outcome
MH  - Viral Vaccines/*administration & dosage/*immunology
MH  - Viremia/prevention & control
MH  - Virus Shedding
OTO - NOTNLM
OT  - Efficacy
OT  - PRRSV
OT  - Porcine reproductive and respiratory syndrome virus
OT  - Vaccine
EDAT- 2016/11/24 06:00
MHDA- 2017/12/16 06:00
CRDT- 2016/11/24 06:00
PHST- 2016/08/30 00:00 [received]
PHST- 2016/10/21 00:00 [revised]
PHST- 2016/11/07 00:00 [accepted]
PHST- 2016/11/24 06:00 [pubmed]
PHST- 2017/12/16 06:00 [medline]
PHST- 2016/11/24 06:00 [entrez]
AID - S0264-410X(16)31067-2 [pii]
AID - 10.1016/j.vaccine.2016.11.020 [doi]
PST - ppublish
SO  - Vaccine. 2017 Jan 3;35(1):125-131. doi: 10.1016/j.vaccine.2016.11.020. Epub 2016 
      Nov 18.