PMID- 27876461
OWN - NLM
STAT- MEDLINE
DCOM- 20170605
LR  - 20190221
IS  - 1521-7035 (Electronic)
IS  - 1521-6616 (Linking)
VI  - 175
DP  - 2017 Feb
TI  - Neutrophil infiltration mediated by CXCL5 accumulation in the laryngeal squamous 
      cell carcinoma microenvironment: A mechanism by which tumour cells escape immune 
      surveillance.
PG  - 34-40
LID - S1521-6616(16)30634-9 [pii]
LID - 10.1016/j.clim.2016.11.009 [doi]
AB  - The CXCL5 chemokine is important for neutrophil accumulation in tumour tissues. 
      In this report, we attempted to clarify whether and how infiltrating 
      tumour-associated neutrophils (TANs) in laryngeal squamous cell carcinoma (LSCC) 
      affect the proliferation and activation of T cells. We examined chemokine 
      expression by real-time PCR (RT-PCR) and enzyme-linked immunosorbent assay 
      (ELISA) and performed an immunohistochemical analysis of LSCC microarrays. The 
      relationship between CXCL5 and CD66b (a neutrophil marker) was investigated by 
      immunofluorescence staining. We found that CXCL5 was upregulated in LSCC tissues, 
      whereas CXCL5 levels were decreased in LSCC patient serum. Furthermore, high 
      levels of CXCL5 were significantly correlated with intratumoural neutrophil 
      infiltration. Compared with peripheral blood neutrophils (PBNs), TANs 
      significantly inhibited T cell proliferation and decreased IFN-gamma and TNF-alpha 
      secretion. These data suggest that excessive neutrophil infiltration is 
      associated with advanced clinical stages of LSCC (T3 or T4, III or IV, and N1 or 
      N2).
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Zhang, Duo
AU  - Zhang D
AD  - Department of Otolaryngology-HNS, Eye, Ear, Nose and Throat Hospital, Shanghai 
      Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of 
      Medicine, 83 Fenyang Road, Shanghai 200031, China; Department of Pudong Hospital, 
      Fudan University School of Medicine, 2800 Gongwei Road, Shanghai 201300, China.
FAU - Zhou, Jian
AU  - Zhou J
AD  - Department of Otolaryngology-HNS, Eye, Ear, Nose and Throat Hospital, Shanghai 
      Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of 
      Medicine, 83 Fenyang Road, Shanghai 200031, China; Department of Pudong Hospital, 
      Fudan University School of Medicine, 2800 Gongwei Road, Shanghai 201300, China.
FAU - Tang, Di
AU  - Tang D
AD  - Department of Otolaryngology-HNS, Eye, Ear, Nose and Throat Hospital, Shanghai 
      Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of 
      Medicine, 83 Fenyang Road, Shanghai 200031, China; Department of Pudong Hospital, 
      Fudan University School of Medicine, 2800 Gongwei Road, Shanghai 201300, China.
FAU - Zhou, Lin
AU  - Zhou L
AD  - Shanghai Institute of Immunology, Shanghai Jiaotong University School of 
      Medicine, 280 South Chongqing Road, Shanghai 200025, China.
FAU - Chou, Liang
AU  - Chou L
AD  - Department of Otolaryngology-HNS, Eye, Ear, Nose and Throat Hospital, Shanghai 
      Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of 
      Medicine, 83 Fenyang Road, Shanghai 200031, China; Department of Pudong Hospital, 
      Fudan University School of Medicine, 2800 Gongwei Road, Shanghai 201300, China.
FAU - Chou, Kuang-Yen
AU  - Chou KY
AD  - Shanghai Institute of Immunology, Shanghai Jiaotong University School of 
      Medicine, 280 South Chongqing Road, Shanghai 200025, China.
FAU - Tao, Lei
AU  - Tao L
AD  - Department of Otolaryngology-HNS, Eye, Ear, Nose and Throat Hospital, Shanghai 
      Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of 
      Medicine, 83 Fenyang Road, Shanghai 200031, China; Department of Pudong Hospital, 
      Fudan University School of Medicine, 2800 Gongwei Road, Shanghai 201300, China. 
      Electronic address: doctortaolei@163.com.
FAU - Lu, Li-Ming
AU  - Lu LM
AD  - Shanghai Institute of Immunology, Shanghai Jiaotong University School of 
      Medicine, 280 South Chongqing Road, Shanghai 200025, China. Electronic address: 
      lulunew2003@163.com.
LA  - eng
PT  - Journal Article
DEP - 20161119
PL  - United States
TA  - Clin Immunol
JT  - Clinical immunology (Orlando, Fla.)
JID - 100883537
RN  - 0 (CXCL5 protein, human)
RN  - 0 (Chemokine CXCL5)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/blood/*immunology/metabolism
MH  - Cell Proliferation/physiology
MH  - Chemokine CXCL5/blood/*metabolism
MH  - Female
MH  - Head and Neck Neoplasms/blood/immunology/metabolism
MH  - Humans
MH  - Laryngeal Neoplasms/blood/*immunology/metabolism
MH  - Male
MH  - Middle Aged
MH  - Neutrophil Infiltration/*immunology
MH  - Neutrophils/*immunology/metabolism
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - T-Lymphocytes/immunology/metabolism
MH  - Tumor Microenvironment/*immunology
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Up-Regulation/immunology
OTO - NOTNLM
OT  - CXCL5
OT  - LSCC
OT  - Neutrophil
OT  - PBNs
OT  - TANs
OT  - Tumour microenvironment
EDAT- 2016/11/24 06:00
MHDA- 2017/06/06 06:00
CRDT- 2016/11/24 06:00
PHST- 2016/04/24 00:00 [received]
PHST- 2016/11/17 00:00 [revised]
PHST- 2016/11/18 00:00 [accepted]
PHST- 2016/11/24 06:00 [pubmed]
PHST- 2017/06/06 06:00 [medline]
PHST- 2016/11/24 06:00 [entrez]
AID - S1521-6616(16)30634-9 [pii]
AID - 10.1016/j.clim.2016.11.009 [doi]
PST - ppublish
SO  - Clin Immunol. 2017 Feb;175:34-40. doi: 10.1016/j.clim.2016.11.009. Epub 2016 Nov 
      19.