PMID- 27876873
OWN - NLM
STAT- MEDLINE
DCOM- 20180521
LR  - 20211204
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Nov 23
TI  - The Effect of Silica Nanoparticles on Human Corneal Epithelial Cells.
PG  - 37762
LID - 10.1038/srep37762 [doi]
LID - 37762
AB  - Ocular drug delivery is an interesting field in current research. Silica 
      nanoparticles (SiNPs) are promising drug carriers for ophthalmic drug delivery. 
      However, little is known about the toxicity of SiNPs on ocular surface cells such 
      as human corneal epithelial cells (HCECs). In this study, we evaluated the 
      cytotoxicity induced by 50, 100 and 150 nm sizes of SiNPs on cultured HCECs for 
      up to 48 hours. SiNPs were up-taken by HCECs inside cytoplasmic vacuoles. 
      Cellular reactive oxygen species generation was mildly elevated, dose 
      dependently, with SiNPs, but no significant decrease of cellular viability was 
      observed up to concentrations of 100 mug/ml for three different sized SiNPs. 
      Western blot assays revealed that both cellular autophagy and mammalian target of 
      rapamycin (mTOR) pathways were activated with the addition of SiNPs. Our findings 
      suggested that 50, 100 and 150 nm sized SiNPs did not induce significant 
      cytotoxicity in cultured HCECs.
FAU - Park, Joo-Hee
AU  - Park JH
AD  - Department of Ophthalmology, Dongguk University, Ilsan Hospital, Goyang, South 
      Korea.
FAU - Jeong, Hyejoong
AU  - Jeong H
AD  - School of Chemical Engineering and Material Science, Chung-Ang University, Seoul, 
      South Korea.
FAU - Hong, Jinkee
AU  - Hong J
AD  - School of Chemical Engineering and Material Science, Chung-Ang University, Seoul, 
      South Korea.
FAU - Chang, Minwook
AU  - Chang M
AD  - Department of Ophthalmology, Dongguk University, Ilsan Hospital, Goyang, South 
      Korea.
FAU - Kim, Martha
AU  - Kim M
AD  - Department of Ophthalmology, Dongguk University, Ilsan Hospital, Goyang, South 
      Korea.
FAU - Chuck, Roy S
AU  - Chuck RS
AD  - Department of Ophthalmology and Visual Sciences, Montefiore Medical Center, 
      Albert Einstein College of Medicine, Bronx, NY, USA.
FAU - Lee, Jimmy K
AU  - Lee JK
AD  - Department of Ophthalmology and Visual Sciences, Montefiore Medical Center, 
      Albert Einstein College of Medicine, Bronx, NY, USA.
FAU - Park, Choul-Yong
AU  - Park CY
AD  - Department of Ophthalmology, Dongguk University, Ilsan Hospital, Goyang, South 
      Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161123
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Reactive Oxygen Species)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Apoptosis/drug effects
MH  - Autophagy/drug effects
MH  - Cell Survival/drug effects
MH  - Epithelial Cells/*cytology/drug effects/metabolism
MH  - Epithelium, Corneal/*cytology
MH  - Glutathione/metabolism
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Intracellular Space/metabolism
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Nanoparticles/*toxicity/ultrastructure
MH  - Oxidative Stress/drug effects
MH  - Particle Size
MH  - Reactive Oxygen Species/metabolism
MH  - Signal Transduction/drug effects
MH  - Silicon Dioxide/*toxicity
MH  - Static Electricity
MH  - TOR Serine-Threonine Kinases/metabolism
PMC - PMC5120337
EDAT- 2016/11/24 06:00
MHDA- 2018/05/22 06:00
PMCR- 2016/11/23
CRDT- 2016/11/24 06:00
PHST- 2016/08/05 00:00 [received]
PHST- 2016/11/01 00:00 [accepted]
PHST- 2016/11/24 06:00 [entrez]
PHST- 2016/11/24 06:00 [pubmed]
PHST- 2018/05/22 06:00 [medline]
PHST- 2016/11/23 00:00 [pmc-release]
AID - srep37762 [pii]
AID - 10.1038/srep37762 [doi]
PST - epublish
SO  - Sci Rep. 2016 Nov 23;6:37762. doi: 10.1038/srep37762.