PMID- 27877662
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240326
IS  - 1468-6996 (Print)
IS  - 1878-5514 (Electronic)
IS  - 1468-6996 (Linking)
VI  - 15
IP  - 2
DP  - 2014 Apr
TI  - Modulation of human dermal microvascular endothelial cell and human gingival 
      fibroblast behavior by micropatterned silica coating surfaces for zirconia dental 
      implant applications.
PG  - 025001
LID - 025001
AB  - Dental ceramic implants have shown superior esthetic behavior and the absence of 
      induced allergic disorders when compared to titanium implants. Zirconia may 
      become a potential candidate to be used as an alternative to titanium dental 
      implants if surface modifications are introduced. In this work, bioactive 
      micropatterned silica coatings were produced on zirconia substrates, using a 
      combined methodology of sol-gel processing and soft lithography. The aim of the 
      work was to compare the in vitro behavior of human gingival fibroblasts (HGFs) 
      and human dermal microvascular endothelial cells (HDMECs) on three types of 
      silica-coated zirconia surfaces: flat and micropatterned (with pillars and with 
      parallel grooves). Our results showed that cells had a higher metabolic activity 
      (HGF, HDMEC) and increased gene expression levels of fibroblast-specific 
      protein-1 (FSP-1) and collagen type I (COL I) on surfaces with pillars. 
      Nevertheless, parallel grooved surfaces were able to guide cell growth. Even 
      capillary tube-like networks of HDMEC were oriented according to the surface 
      geometry. Zirconia and silica with different topographies have shown to be blood 
      compatible and silica coating reduced bacteria adhesion. All together, the 
      results indicated that microstructured bioactive coating seems to be an efficient 
      strategy to improve soft tissue integration on zirconia implants, protecting 
      implants from peri-implant inflammation and improving long-term implant 
      stabilization. This new approach of micropatterned silica coating on zirconia 
      substrates can generate promising novel dental implants, with surfaces that 
      provide physical cues to guide cells and enhance their behavior.
FAU - Laranjeira, Marta S
AU  - Laranjeira MS
AD  - INEB-Instituto Engenharia Biomedica, Universidade do Porto, Porto, Portugal; 
      Faculdade de Engenharia, DEMM, Universidade do Porto, Porto, Portugal.
FAU - Carvalho, Angela
AU  - Carvalho A
AD  - INEB-Instituto Engenharia Biomedica, Universidade do Porto, Porto, Portugal; 
      Faculdade de Engenharia, DEMM, Universidade do Porto, Porto, Portugal.
FAU - Pelaez-Vargas, Alejandro
AU  - Pelaez-Vargas A
AD  - Facultad de Odontologia, Universidad Cooperativa de Colombia, Medellin, Colombia.
FAU - Hansford, Derek
AU  - Hansford D
AD  - Department of Biomedical Engineering (BME), Ohio State University, Columbus, OH, 
      USA.
FAU - Ferraz, Maria Pia
AU  - Ferraz MP
AD  - INEB-Instituto Engenharia Biomedica, Universidade do Porto, Porto, Portugal; 
      Laboratory CEBIMED-Centro de Estudos em Biomedicina, Universidade Fernando 
      Pessoa, Porto, Portugal.
FAU - Coimbra, Susana
AU  - Coimbra S
AD  - IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, 
      Portugal; CESPU-Instituto de Investigacao e Formacao Avancada em Ciencias e 
      Tecnologias da Saude, Gandra-PRD, Portugal.
FAU - Costa, Elisio
AU  - Costa E
AD  - IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, 
      Portugal; Departamento de Ciencias Biologicas-Servico de Bioquimica, Faculdade de 
      Farmacia, Universidade do Porto, Porto, Portugal.
FAU - Santos-Silva, Alice
AU  - Santos-Silva A
AD  - IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, 
      Portugal; Departamento de Ciencias Biologicas-Servico de Bioquimica, Faculdade de 
      Farmacia, Universidade do Porto, Porto, Portugal.
FAU - Fernandes, Maria Helena
AU  - Fernandes MH
AD  - Laboratory for Bone Metabolism and Regeneration, Faculdade de Medicina Dentaria, 
      Universidade do Porto, Porto, Portugal.
FAU - Monteiro, Fernando Jorge
AU  - Monteiro FJ
AD  - INEB-Instituto Engenharia Biomedica, Universidade do Porto, Porto, Portugal; 
      Faculdade de Engenharia, DEMM, Universidade do Porto, Porto, Portugal.
LA  - eng
PT  - Journal Article
DEP - 20140307
PL  - United States
TA  - Sci Technol Adv Mater
JT  - Science and technology of advanced materials
JID - 101614420
PMC - PMC5090413
OTO - NOTNLM
OT  - Bacteria adhesion
OT  - Biomaterials
OT  - Hemolysis
OT  - Human dermal microvascular endothelial cells (HDMECs)
OT  - Human gingival fibroblasts (HGFs)
OT  - Micropatterning
OT  - Soft lithography
EDAT- 2014/03/07 00:00
MHDA- 2014/03/07 00:01
PMCR- 2014/03/07
CRDT- 2016/11/24 06:00
PHST- 2013/04/19 00:00 [received]
PHST- 2014/03/07 00:00 [revised]
PHST- 2014/02/09 00:00 [accepted]
PHST- 2016/11/24 06:00 [entrez]
PHST- 2014/03/07 00:00 [pubmed]
PHST- 2014/03/07 00:01 [medline]
PHST- 2014/03/07 00:00 [pmc-release]
AID - TSTA11661157 [pii]
AID - 10.1088/1468-6996/15/2/025001 [doi]
PST - epublish
SO  - Sci Technol Adv Mater. 2014 Mar 7;15(2):025001. doi: 
      10.1088/1468-6996/15/2/025001. eCollection 2014 Apr.