PMID- 27878278
OWN - NLM
STAT- MEDLINE
DCOM- 20170406
LR  - 20181119
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 14
IP  - 6
DP  - 2016 Dec
TI  - Alteration of epoxyeicosatrienoic acids in the liver and kidney of cytochrome 
      P450 4F2 transgenic mice.
PG  - 5739-5745
LID - 10.3892/mmr.2016.5962 [doi]
AB  - Arachidonic acid (AA) can be metabolized into 20-hydroxyeicosatetraenoic acid 
      (20-HETE) by omega-hydroxylases, and epoxyeicosatrienoic acids (EETs) by 
      epoxygenases. The effects of EETs in cardiovascular physiology are vasodilatory, 
      anti-inflammatory and anti‑apoptotic, which are opposite to the function to 
      20‑HETE. However, EETs are not stable in vivo, and are rapidly degraded to the 
      biologically less active metabolites, dihydroxyeicosatrienoic acids, via soluble 
      epoxide hydrolase (sEH). Western blotting, reverse transcription‑quantitative 
      polymerase chain reaction and liquid chromatography tandem mass spectrometry were 
      performed in order to determine target RNA and protein expression levels. In the 
      present study, it was demonstrated that the disturbed renal 20‑HETE/EET ratio in 
      the hypertensive cytochrome P450 4F2 transgenic mice was caused by the activation 
      of sEH and the repression of epoxygenase activity. In addition, 20‑HETE showed an 
      opposite regulatory effect on the endogenous epoxygenases in the liver and 
      kidney. Given that 20‑HETE and EETs have opposite effects in multiple disease, 
      the regulation of their formation and degradation may yield therapeutic benefits.
FAU - Zhang, Bijun
AU  - Zhang B
AD  - Department of Clinical Genetics, Shengjing Hospital of China Medical University, 
      Shenyang, Liaoning 110004, P.R. China.
FAU - Lai, Guangrui
AU  - Lai G
AD  - Department of Clinical Genetics, Shengjing Hospital of China Medical University, 
      Shenyang, Liaoning 110004, P.R. China.
FAU - Liu, Xiaoliang
AU  - Liu X
AD  - Department of Clinical Genetics, Shengjing Hospital of China Medical University, 
      Shenyang, Liaoning 110004, P.R. China.
FAU - Zhao, Yanyan
AU  - Zhao Y
AD  - Department of Clinical Genetics, Shengjing Hospital of China Medical University, 
      Shenyang, Liaoning 110004, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20161123
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Eicosanoids)
RN  - 0 (Isoenzymes)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.14.14.1 (Cytochrome P450 Family 4)
RN  - EC 1.14.14.78 (CYP4F2 protein, human)
RN  - EC 3.3.2.- (Epoxide Hydrolases)
RN  - EC 3.3.2.10 (EPHX2 protein, human)
SB  - IM
MH  - Animals
MH  - Arachidonic Acid/*metabolism
MH  - Chromatography, Liquid
MH  - Cytochrome P450 Family 4/*genetics/metabolism
MH  - Eicosanoids/blood/metabolism
MH  - Epoxide Hydrolases/genetics/metabolism
MH  - Gene Expression Profiling
MH  - Humans
MH  - Hydroxylation
MH  - Isoenzymes
MH  - Kidney/*drug effects/*metabolism
MH  - Liver/*drug effects/*metabolism
MH  - Male
MH  - Metabolic Syndrome/genetics/metabolism
MH  - Metabolomics/methods
MH  - Mice
MH  - Mice, Transgenic
MH  - Middle Aged
MH  - Oxidative Stress/genetics
MH  - Oxidoreductases/metabolism
MH  - Tandem Mass Spectrometry
EDAT- 2016/11/24 06:00
MHDA- 2017/04/07 06:00
CRDT- 2016/11/24 06:00
PHST- 2015/09/15 00:00 [received]
PHST- 2016/09/26 00:00 [accepted]
PHST- 2016/11/24 06:00 [pubmed]
PHST- 2017/04/07 06:00 [medline]
PHST- 2016/11/24 06:00 [entrez]
AID - 10.3892/mmr.2016.5962 [doi]
PST - ppublish
SO  - Mol Med Rep. 2016 Dec;14(6):5739-5745. doi: 10.3892/mmr.2016.5962. Epub 2016 Nov 
      23.