PMID- 27881156
OWN - NLM
STAT- MEDLINE
DCOM- 20170413
LR  - 20211203
IS  - 0717-6287 (Electronic)
IS  - 0716-9760 (Print)
IS  - 0716-9760 (Linking)
VI  - 49
IP  - 1
DP  - 2016 Nov 24
TI  - Klotho sensitive regulation of dendritic cell functions by vitamin E.
PG  - 45
LID - 45
AB  - BACKGROUND: Dendritic cells (DCs) are the most potent professional 
      antigen-presenting cells for naive T cells to link innate and acquired immunity. 
      Klotho, an anti-aging protein, participates in the regulation of Ca(2+) dependent 
      migration in DCs. Vitamin E (VitE) is an essential antioxidant to protect cells 
      from damage and elicits its inhibitory effects on NF-kappaB-mediated inflammatory 
      response. However, the roles of VitE on mouse DC functions and the contribution 
      of klotho to those effects both are unknown. The present study explored the 
      effects of VitE on klotho expression, maturation, ROS production and migration in 
      DCs. METHODS: The mouse bone marrow cells were isolated and cultured with GM-CSF 
      to attain bone marrow-derived DCs (BMDCs). Cells were stimulated with LPS 
      (100 ng/ml) in the presence or absence of VitE (500 microM). RT-PCR and 
      immunoprecipitation methods were employed to determine klotho expression, ELISA 
      to determine cytokine release, flow cytometry to analyze number of 
      CD86(+)CD11c(+) cells, the intracellular expression of cytokines and reactive 
      oxygen species (ROS) production and a transwell migration assay to trace 
      migration. RESULTS: Klotho transcript level and this hormone secretion in DC 
      supernatant were enhanced by VitE treatment and further increased in the presence 
      of NF-kappaB inhibitor Bay 11-7082 (10 microM). Moreover, VitE treatment inhibited 
      IL-12p70 protein expression of, ROS accumulation in and CCL21-dependent migration 
      of LPS-triggered mature DCs, these effects were reversed following klotho 
      silencing. CONCLUSION: The up-regulation of klotho by VitE could contribute to 
      the inhibitory effects of VitE on NF-kappaB-mediated DC functional maturation. The 
      events might contribute to immunotherapeutic effect of VitE on the 
      pathophysiology of klotho-related disease.
FAU - Xuan, Nguyen Thi
AU  - Xuan NT
AD  - Institute of Genome Research, Vietnam Academy of Science and Technology, 18 Hoang 
      Quoc Viet, Cau Giay, Hanoi, Vietnam. xuannt@igr.ac.vn.
FAU - Trang, Phi Thi Thu
AU  - Trang PT
AD  - Institute of Genome Research, Vietnam Academy of Science and Technology, 18 Hoang 
      Quoc Viet, Cau Giay, Hanoi, Vietnam.
FAU - Van Phong, Nguyen
AU  - Van Phong N
AD  - Institute of Genome Research, Vietnam Academy of Science and Technology, 18 Hoang 
      Quoc Viet, Cau Giay, Hanoi, Vietnam.
FAU - Toan, Nguyen Linh
AU  - Toan NL
AD  - Department of Pathophysiology, Vietnam Military Medical University, Ha Dong, 
      Hanoi, Vietnam.
FAU - Trung, Do Minh
AU  - Trung DM
AD  - Department of Protein-Toxic-Cells, Biomedical & Pharmaceutical Applied Research 
      Center, Vietnam Military Medical University, Ha Dong, Hanoi, Vietnam.
FAU - Bac, Nguyen Duy
AU  - Bac ND
AD  - Vietnam Military Medical University, Ha Dong, Hanoi, Vietnam.
FAU - Nguyen, Viet Linh
AU  - Nguyen VL
AD  - Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang 
      Quoc Viet, Cau Giay, Hanoi, Vietnam.
FAU - Hoang, Nguyen Huy
AU  - Hoang NH
AD  - Institute of Genome Research, Vietnam Academy of Science and Technology, 18 Hoang 
      Quoc Viet, Cau Giay, Hanoi, Vietnam.
FAU - Van Hai, Nong
AU  - Van Hai N
AD  - Institute of Genome Research, Vietnam Academy of Science and Technology, 18 Hoang 
      Quoc Viet, Cau Giay, Hanoi, Vietnam.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20161124
PL  - England
TA  - Biol Res
JT  - Biological research
JID - 9308271
RN  - 0 (IkappaBNS protein, mouse)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Proteins)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vitamins)
RN  - 1406-18-4 (Vitamin E)
RN  - 187348-17-0 (Interleukin-12)
RN  - EC 3.2.1.31 (Glucuronidase)
RN  - EC 3.2.1.31 (Klotho Proteins)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - Cell Movement/drug effects
MH  - Dendritic Cells/*drug effects/physiology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flow Cytometry
MH  - Glucuronidase/*drug effects/physiology
MH  - Immunoblotting
MH  - Interleukin-12/analysis
MH  - Intracellular Signaling Peptides and Proteins
MH  - Klotho Proteins
MH  - Lipopolysaccharides
MH  - Mice, Inbred BALB C
MH  - Proteins/analysis/drug effects
MH  - Reactive Oxygen Species/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Reproducibility of Results
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/analysis
MH  - Up-Regulation
MH  - Vitamin E/*pharmacology
MH  - Vitamins/*pharmacology
PMC - PMC5121936
OTO - NOTNLM
OT  - Dendritic cells
OT  - Klotho
OT  - LPS
OT  - Migration
OT  - ROS
OT  - Vitamin E
EDAT- 2016/11/25 06:00
MHDA- 2017/04/14 06:00
PMCR- 2016/11/24
CRDT- 2016/11/25 06:00
PHST- 2016/08/09 00:00 [received]
PHST- 2016/11/17 00:00 [accepted]
PHST- 2016/11/25 06:00 [entrez]
PHST- 2016/11/25 06:00 [pubmed]
PHST- 2017/04/14 06:00 [medline]
PHST- 2016/11/24 00:00 [pmc-release]
AID - 10.1186/s40659-016-0105-4 [pii]
AID - 105 [pii]
AID - 10.1186/s40659-016-0105-4 [doi]
PST - epublish
SO  - Biol Res. 2016 Nov 24;49(1):45. doi: 10.1186/s40659-016-0105-4.