PMID- 27881436
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 2046-6390 (Print)
IS  - 2046-6390 (Electronic)
IS  - 2046-6390 (Linking)
VI  - 6
IP  - 1
DP  - 2017 Jan 15
TI  - Knockdown of histidine-rich calcium-binding protein (HRC) suppresses liver 
      fibrosis by inhibiting the activation of hepatic stellate cells.
PG  - 29-34
LID - 10.1242/bio.019828 [doi]
AB  - The histidine-rich calcium-binding protein (HRC) is a regulator of Ca(2+) 
      homeostasis and it plays a significant role in hepatocellular carcinoma (HCC) 
      progression. However, the relationship between HRC and liver fibrogenesis is 
      still unknown. Our data demonstrates that HRC was upregulated in fibrotic liver 
      and activated hepatic stellate cells (HSCs). TGF-beta treatment increased alpha-SMA and 
      HRC expression dose-dependently in HSCs. Repression of HRC reduced alpha-SMA, CTGF 
      and collagen expression, and inhibited HSC proliferation and migration. In 
      addition, we found that the anti-fibrosis effect of HRC knockdown was associated 
      with endoplasmic reticulum (ER) stress. Silencing of HRC decreased the expression 
      of ER stress and autophagy markers. Moreover, ER stress agonist thapsigargin (TG) 
      enhanced, whereas ER stress antagonist 4-phenylbutyric acid (4-PBA) alleviated 
      HSCs activation and autophagy. In conclusion, these data indicate that depletion 
      of HRC inhibited HSC activation through the ER stress pathway, and HRC may be a 
      potential regulator of liver fibrosis.
CI  - (c) 2017. Published by The Company of Biologists Ltd.
FAU - Liu, Jingmei
AU  - Liu J
AD  - Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430030, China.
FAU - Li, Mengke
AU  - Li M
AD  - Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430030, China.
AD  - Department of Gastroenterology, Zhoushan Hospital, Zhoushan 316000, China.
FAU - Gong, Jin
AU  - Gong J
AD  - Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430030, China.
FAU - Han, Ping
AU  - Han P
AD  - Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430030, China.
FAU - Wang, Yunwu
AU  - Wang Y
AD  - Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430030, China.
FAU - Li, Dongxiao
AU  - Li D
AD  - Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430030, China.
FAU - Tian, Dean
AU  - Tian D
AUID- ORCID: 0000-0002-1407-6351
AD  - Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430030, China datian@tjh.tjmu.edu.cn 
      liaojiazhi2011@163.com.
FAU - Liao, Jiazhi
AU  - Liao J
AD  - Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430030, China datian@tjh.tjmu.edu.cn 
      liaojiazhi2011@163.com.
LA  - eng
PT  - Journal Article
DEP - 20170115
PL  - England
TA  - Biol Open
JT  - Biology open
JID - 101578018
PMC - PMC5278420
OTO - NOTNLM
OT  - ER stress
OT  - HRC
OT  - Hepatic stellate cells
OT  - Liver fibrosis
COIS- The authors declare no competing or financial interests.
EDAT- 2016/11/25 06:00
MHDA- 2016/11/25 06:01
PMCR- 2016/11/23
CRDT- 2016/11/25 06:00
PHST- 2016/11/25 06:00 [pubmed]
PHST- 2016/11/25 06:01 [medline]
PHST- 2016/11/25 06:00 [entrez]
PHST- 2016/11/23 00:00 [pmc-release]
AID - bio.019828 [pii]
AID - BIO019828 [pii]
AID - 10.1242/bio.019828 [doi]
PST - epublish
SO  - Biol Open. 2017 Jan 15;6(1):29-34. doi: 10.1242/bio.019828.