PMID- 2788417
OWN - NLM
STAT- MEDLINE
DCOM- 19890921
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 162
IP  - 3
DP  - 1989 Aug 15
TI  - Functional similarity of HIV-I rev and HTLV-I rex proteins: identification of a 
      new nucleolar-targeting signal in rev protein.
PG  - 963-70
AB  - We have tested the functional compatibility between rev protein of human 
      immunodeficiency virus type I (HIV-I) and rex protein of human T-cell 
      lymphotropic virus type I (HTLV-I). Each protein recognized the other's 
      cis-acting sequence, albeit at reduced levels. Both proteins localize 
      predominantly in the nucleolus. We have identified a new nucleolar-targeting 
      signal in rev protein, which was homologous to that of rex protein. The sequence 
      [35-RQARRNRRRRWRERQR-50] in rev protein, when fused to the amino-terminus of 
      beta-galactosidase, directed the hybrid protein to the cell nucleolus. A deletion 
      mutant which lacks several amino acid residues within the signal failed to 
      function in the CAT assay system. These results demonstrate that the nucleolar 
      targeting signals are essential for the functions of Rev and Rex.
FAU - Kubota, S
AU  - Kubota S
AD  - Institute for Virus Research, Kyoto University, Japan.
FAU - Siomi, H
AU  - Siomi H
FAU - Satoh, T
AU  - Satoh T
FAU - Endo, S
AU  - Endo S
FAU - Maki, M
AU  - Maki M
FAU - Hatanaka, M
AU  - Hatanaka M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA, Recombinant)
RN  - 0 (Gene Products, rev)
RN  - 0 (HTLV-I Antigens)
RN  - 0 (Retroviridae Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (Viral Proteins)
RN  - 0 (rev Gene Products, Human Immunodeficiency Virus)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cell Compartmentation
MH  - Cell Nucleolus
MH  - DNA Mutational Analysis
MH  - DNA, Recombinant
MH  - *Gene Expression Regulation
MH  - Gene Products, rev
MH  - *Genes, Viral
MH  - HIV/*physiology
MH  - HTLV-I Antigens/*physiology
MH  - Human T-lymphotropic virus 1/*physiology
MH  - Molecular Sequence Data
MH  - Plasmids
MH  - Regulatory Sequences, Nucleic Acid
MH  - Retroviridae Proteins/*physiology
MH  - Structure-Activity Relationship
MH  - Trans-Activators
MH  - Transcription Factors/*physiology
MH  - Viral Proteins/*physiology
MH  - rev Gene Products, Human Immunodeficiency Virus
EDAT- 1989/08/15 00:00
MHDA- 1989/08/15 00:01
CRDT- 1989/08/15 00:00
PHST- 1989/08/15 00:00 [pubmed]
PHST- 1989/08/15 00:01 [medline]
PHST- 1989/08/15 00:00 [entrez]
AID - 0006-291X(89)90767-5 [pii]
AID - 10.1016/0006-291x(89)90767-5 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1989 Aug 15;162(3):963-70. doi: 
      10.1016/0006-291x(89)90767-5.