PMID- 27889163
OWN - NLM
STAT- MEDLINE
DCOM- 20170306
LR  - 20170828
IS  - 1095-9998 (Electronic)
IS  - 0740-0020 (Linking)
VI  - 62
DP  - 2017 Apr
TI  - Differential detection of pathogenic Yersinia spp. by fluorescence in situ 
      hybridization.
PG  - 39-45
LID - S0740-0020(15)30216-1 [pii]
LID - 10.1016/j.fm.2016.09.013 [doi]
AB  - Yersinia enterocolitica, Y. pseudotuberculosis and Y. pestis are pathogens of 
      major medical importance, which are responsible for a considerable number of 
      infections every year. The detection of these species still relies on cultural 
      methods, which are slow, labour intensive and often hampered by the presence of 
      high amounts of accompanying flora. In this study, fluorescence in situ 
      hybridization (FISH) was used to develop a fast, sensitive and reliable 
      alternative to detect viable bacteria in food. For this purpose, highly specific 
      probes targeting the 16S and 23S ribosomal RNA were employed to differentially 
      detect each of the three species. In order to enable the differentiation of 
      single nucleotide polymorphisms (SNPs), suitable competitor oligonucleotides and 
      locked nucleic acids (LNAs) were used. Starved cells still showed a strong signal 
      and a direct viable count (DVC) approach combined with FISH optimized live/dead 
      discrimination. Sensitivity of the FISH test was high and even a single cell per 
      gram of spiked minced pork meat could be detected within a day, demonstrating the 
      applicability to identify foodborne hazards at an early stage. In conclusion, the 
      established FISH tests proved to be promising tools to compensate existing 
      drawbacks of the conventional cultural detection of these important zoonotic 
      agents.
CI  - Copyright A(c) 2016 Elsevier Ltd. All rights reserved.
FAU - Rohde, Alexander
AU  - Rohde A
AD  - German Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, 
      Germany; Free University Berlin, Department of Biology, Chemistry and Pharmacy, 
      Takustr. 3, 14195 Berlin, Germany. Electronic address: 
      alexander.rohde@bfr.bund.de.
FAU - Hammerl, Jens Andre
AU  - Hammerl JA
AD  - German Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, 
      Germany.
FAU - Appel, Bernd
AU  - Appel B
AD  - German Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, 
      Germany.
FAU - Dieckmann, Ralf
AU  - Dieckmann R
AD  - German Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, 
      Germany.
FAU - Al Dahouk, Sascha
AU  - Al Dahouk S
AD  - German Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, 
      Germany.
LA  - eng
PT  - Journal Article
DEP - 20160921
PL  - England
TA  - Food Microbiol
JT  - Food microbiology
JID - 8601127
RN  - 0 (RNA Probes)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (RNA, Ribosomal, 23S)
SB  - IM
MH  - Bacteria/genetics
MH  - Bacterial Load
MH  - Food Microbiology
MH  - Food Safety/*methods
MH  - In Situ Hybridization, Fluorescence
MH  - Polymorphism, Single Nucleotide/immunology
MH  - RNA Probes
MH  - RNA, Ribosomal, 16S
MH  - RNA, Ribosomal, 23S
MH  - Red Meat/microbiology
MH  - Sensitivity and Specificity
MH  - Yersinia enterocolitica/genetics/*isolation & purification
MH  - Yersinia pestis/genetics/*isolation & purification
MH  - Yersinia pseudotuberculosis/genetics/*isolation & purification
OTO - NOTNLM
OT  - Direct viable count
OT  - FISH
OT  - Food safety
OT  - Yersinia
EDAT- 2016/11/28 06:00
MHDA- 2017/03/07 06:00
CRDT- 2016/11/28 06:00
PHST- 2015/12/14 00:00 [received]
PHST- 2016/09/16 00:00 [revised]
PHST- 2016/09/18 00:00 [accepted]
PHST- 2016/11/28 06:00 [entrez]
PHST- 2016/11/28 06:00 [pubmed]
PHST- 2017/03/07 06:00 [medline]
AID - S0740-0020(15)30216-1 [pii]
AID - 10.1016/j.fm.2016.09.013 [doi]
PST - ppublish
SO  - Food Microbiol. 2017 Apr;62:39-45. doi: 10.1016/j.fm.2016.09.013. Epub 2016 Sep 
      21.