PMID- 27890615
OWN - NLM
STAT- MEDLINE
DCOM- 20170602
LR  - 20180118
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 482
IP  - 4
DP  - 2017 Jan 22
TI  - EGR2 is a gonadotropin-induced survival factor that controls the expression of 
      IER3 in ovarian granulosa cells.
PG  - 877-882
LID - S0006-291X(16)32001-0 [pii]
LID - 10.1016/j.bbrc.2016.11.127 [doi]
AB  - Pituitary gonadotropins are key hormones that orchestrate the growth and 
      development of ovarian follicles. However, limited information is available on 
      intra-ovarian factors that mediate the actions of gonadotropins. In this study, 
      we identified that the early growth response 2 gene (EGR2) is a 
      gonadotropin-inducible gene in granulosa cells of rats and humans. Analysis of 
      consensus EGR-binding elements (EBEs) showed that the immediate early response 3 
      gene (IER3) is a novel transcriptional target gene of EGR2 as confirmed by the 
      luciferase assay, electrophoretic mobility-shift assay (EMSA), chromatin 
      immunoprecipitation (ChIP), and western blot analysis. Overexpression of EGR2 
      promoted survival of KGN human granulosa-derived cells in which IER3 acts as a 
      mediator; knockdown of EGR2 induced death in KGN cells. Additionally, EGR2 was 
      found to regulate the expression of myeloid cell leukemia 1 (MCL-1), which 
      belongs to the BCL-2 family of proteins regulating cell survival. Thus, this 
      study identified a novel signaling axis, comprised of gonadotropins-EGR2-IER3, 
      which is important for the survival of granulosa cells during folliculogenesis.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Jin, Hanyong
AU  - Jin H
AD  - School of Pharmacy, Chung-Ang University, Seoul 06974, South Korea.
FAU - Won, Miae
AU  - Won M
AD  - Department of Pharmacy, CHA University, Seongnam 13488, South Korea.
FAU - Shin, Eunkyoung
AU  - Shin E
AD  - School of Pharmacy, Chung-Ang University, Seoul 06974, South Korea.
FAU - Kim, Hong-Man
AU  - Kim HM
AD  - Department of Life Science, Chung-Ang University, Seoul 06974, South Korea.
FAU - Lee, Kangseok
AU  - Lee K
AD  - Department of Life Science, Chung-Ang University, Seoul 06974, South Korea.
FAU - Bae, Jeehyeon
AU  - Bae J
AD  - School of Pharmacy, Chung-Ang University, Seoul 06974, South Korea. Electronic 
      address: jeehyeon@cau.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20161124
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (EGR2 protein, human)
RN  - 0 (Early Growth Response Protein 2)
RN  - 0 (Egr2 protein, rat)
RN  - 0 (Gonadotropins)
RN  - 0 (IER3 protein, human)
RN  - 0 (IER3 protein, rat)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Apoptosis Regulatory Proteins/*genetics
MH  - Base Sequence
MH  - Cell Line
MH  - Cell Survival
MH  - Early Growth Response Protein 2/*genetics/metabolism
MH  - Female
MH  - Gonadotropins/*metabolism
MH  - Granulosa Cells/cytology/*metabolism
MH  - Humans
MH  - Membrane Proteins/*genetics
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Transcriptional Activation
OTO - NOTNLM
OT  - FSH
OT  - KROX20
OT  - LH
OT  - Target gene
OT  - Transcriptional regulation
EDAT- 2016/11/29 06:00
MHDA- 2017/06/03 06:00
CRDT- 2016/11/29 06:00
PHST- 2016/11/10 00:00 [received]
PHST- 2016/11/23 00:00 [accepted]
PHST- 2016/11/29 06:00 [pubmed]
PHST- 2017/06/03 06:00 [medline]
PHST- 2016/11/29 06:00 [entrez]
AID - S0006-291X(16)32001-0 [pii]
AID - 10.1016/j.bbrc.2016.11.127 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2017 Jan 22;482(4):877-882. doi: 
      10.1016/j.bbrc.2016.11.127. Epub 2016 Nov 24.