PMID- 2789101
OWN - NLM
STAT- MEDLINE
DCOM- 19891017
LR  - 20190919
IS  - 0143-4160 (Print)
IS  - 0143-4160 (Linking)
VI  - 10
IP  - 4
DP  - 1989 May-Jun
TI  - Internal Ca2+ mobilization and secretion in bovine adrenal chromaffin cells.
PG  - 213-21
AB  - Since secretion from intact bovine adrenal chromaffin cells in response to 
      depolarization by nicotine is triggered by a rise in the concentration of 
      intracellular Ca2+ ([Ca2+]i) to about 200-300 nM above basal, it has been assumed 
      that the failure of the inositol 1,4,5-trisphosphate (InsP3)-mobilizing 
      muscarinic agonists to induce secretion reflects the fact that the 50 nM rise in 
      [Ca2+]i they elicit is insufficient to trigger the exocytotic machinery. A recent 
      report, however, has demonstrated that some of the nicotine-induced rise in 
      [Ca2+]i could originate from the InsP3-releasable Ca2+ store. The role of this 
      Ca2+ store in secretion from bovine adrenal chromaffin cells is therefore 
      unclear. In order to investigate in more detail the role of the InsP3-sensitive 
      Ca2+ store in secretion from these cells, we have used a combination of an 
      InsP3-mobilizing muscarinic agonist and the sesquiterpene lactone thapsigargin 
      (TG), which releases internal Ca2+ without concomitant breakdown of inositol 
      lipids or protein kinase C activation, to examine the events which follow 
      depletion of the releasable Ca2+ store in these cells. Monitoring [Ca2+]i using 
      Fura-2 demonstrated that TG released Ca2+ from the InsP3-sensitive store and, 
      additionally, that the Ca2+ response to TG was composed of two distinct, 
      temporally separated, components: a) a slow (1 min) increase in [Ca2+]i to 
      approximately 50 nM above basal that was independent of extracellular Ca2+ and b) 
      the maintenance of this level at a new steady-state that was dependent on the 
      continual entry of extracellular Ca2+.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Cheek, T R
AU  - Cheek TR
AD  - AFRC Unit of Insect Neurophysiology and Pharmacology, Department of Zoology, 
      University of Cambridge, UK.
FAU - Thastrup, O
AU  - Thastrup O
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Cell Calcium
JT  - Cell calcium
JID - 8006226
RN  - 0 (Inositol Phosphates)
RN  - 0 (Methacholine Compounds)
RN  - 0 (Plant Extracts)
RN  - 67526-95-8 (Thapsigargin)
RN  - 6M3C89ZY6R (Nicotine)
RN  - 85166-31-0 (Inositol 1,4,5-Trisphosphate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adrenal Medulla/*metabolism
MH  - Animals
MH  - Calcium/*metabolism
MH  - Cattle
MH  - Dose-Response Relationship, Drug
MH  - Inositol 1,4,5-Trisphosphate
MH  - Inositol Phosphates/pharmacology
MH  - Methacholine Compounds/pharmacology
MH  - Nicotine/pharmacology
MH  - Plant Extracts/pharmacology
MH  - Thapsigargin
EDAT- 1989/05/01 00:00
MHDA- 1989/05/01 00:01
CRDT- 1989/05/01 00:00
PHST- 1989/05/01 00:00 [pubmed]
PHST- 1989/05/01 00:01 [medline]
PHST- 1989/05/01 00:00 [entrez]
AID - 0143-4160(89)90004-3 [pii]
AID - 10.1016/0143-4160(89)90004-3 [doi]
PST - ppublish
SO  - Cell Calcium. 1989 May-Jun;10(4):213-21. doi: 10.1016/0143-4160(89)90004-3.