PMID- 27891557
OWN - NLM
STAT- MEDLINE
DCOM- 20180713
LR  - 20181202
IS  - 1573-6830 (Electronic)
IS  - 0272-4340 (Linking)
VI  - 37
IP  - 7
DP  - 2017 Oct
TI  - Exogenous Expression of Nt-3 and TrkC Genes in Bone Marrow Stromal Cells Elevated 
      the Survival Rate of the Cells in the Course of Neural Differentiation.
PG  - 1187-1194
LID - 10.1007/s10571-016-0448-y [doi]
AB  - Bone marrow stromal cells (BMSCs) are attractive cellular sources for cell 
      therapy of many diseases, specifically neurodegenerative ones. The potential 
      capability of BMSCs could be further augmented by enhancing their neuroprotective 
      property, differentiation potential, and survival rate subsequent to 
      transplantation. Therefore, a concurrent upregulation of neurotrophin-3 (NT-3) 
      and its high affinity receptor, tyrosin kinase C (TrkC), was utilized in our 
      study. BMSCs were cotransfected with pDsRed1-N1-NT-3 and pCMX-TrkC plasmids 
      before induction of neural differentiation. pEGFP-N1-transfected BMSCs were also 
      employed as a control. Real-time quantitative reverse transcription polymerase 
      chain reaction (qRT-PCR) was employed for gene expression analysis. Cell 
      viability was evaluated by MTT assay, while apoptosis rate was assessed by flow 
      cytometry after PI and Annexin V staining. NT-3 and TrkC mRNA levels were greatly 
      elevated following cotransfection of cells with pDsRed1-N1-NT-3 and pCMX-TrkC 
      vectors. The expression of neural markers (i.e., NFM, and NeuroD1) was augmented 
      in cotransfected BMSCs, compared to the control ones, after neural induction. At 
      each time point, the viability and apoptosis rates of the cells over-expressing 
      NT-3 and TrkC showed increased and reduced patterns, respectively. Our data 
      demonstrated that NT-3/TrkC-co-transfected BMSCs, compared to those of intact 
      cells, could be more beneficial graft candidates for the upcoming treatment 
      strategies of neurogenic disorders due to their increased viability and 
      expression of neural markers. This may be due to their increased level of neural 
      differentiation potential and/or their enhanced rate of survival and/or their 
      useful capacity to secrete NT-3.
FAU - Edalat, Houri
AU  - Edalat H
AD  - Genetic Research Center, Baqiyatallah University of Medical Sciences, Tehran, 
      Iran.
FAU - Hajebrahimi, Zahra
AU  - Hajebrahimi Z
AD  - Department of Physiology, Aerospace Research Institute, Tehran, Iran.
FAU - Pirhajati, Vahid
AU  - Pirhajati V
AD  - Department of Anatomy, School of Medicine, Iran University of Medical Sciences, 
      Tehran, Iran.
FAU - Tavallaei, Mahmoud
AU  - Tavallaei M
AD  - Genetic Research Center, Baqiyatallah University of Medical Sciences, Tehran, 
      Iran.
FAU - Movahedin, Mansoureh
AU  - Movahedin M
AD  - Department of Anatomy, School of Medical Sciences, Tarbiat Modares University, 
      Tehran, Iran.
FAU - Mowla, Seyed Javad
AU  - Mowla SJ
AD  - Department of Molecular Genetics, School of Biological Sciences, Tarbiat Modares 
      University, Tehran, Iran. sjmowla@modares.ac.ir.
LA  - eng
GR  - 3657/Basij Elmi Organization/
PT  - Journal Article
DEP - 20161128
PL  - United States
TA  - Cell Mol Neurobiol
JT  - Cellular and molecular neurobiology
JID - 8200709
RN  - 0 (Neurotrophin 3)
RN  - EC 2.7.10.1 (Receptor, trkC)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*physiology
MH  - Cell Survival/physiology
MH  - Cells, Cultured
MH  - Gene Expression
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Neurons/*metabolism
MH  - Neurotrophin 3/*biosynthesis/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkC/*biosynthesis/genetics
OTO - NOTNLM
OT  - Apoptosis
OT  - Bone marrow stromal cells
OT  - Cell viability
OT  - NT-3
OT  - Neural differentiation
OT  - TrkC
EDAT- 2016/11/29 06:00
MHDA- 2018/07/14 06:00
CRDT- 2016/11/29 06:00
PHST- 2016/01/20 00:00 [received]
PHST- 2016/05/04 00:00 [accepted]
PHST- 2016/11/29 06:00 [pubmed]
PHST- 2018/07/14 06:00 [medline]
PHST- 2016/11/29 06:00 [entrez]
AID - 10.1007/s10571-016-0448-y [pii]
AID - 10.1007/s10571-016-0448-y [doi]
PST - ppublish
SO  - Cell Mol Neurobiol. 2017 Oct;37(7):1187-1194. doi: 10.1007/s10571-016-0448-y. 
      Epub 2016 Nov 28.