PMID- 27892877
OWN - NLM
STAT- MEDLINE
DCOM- 20170221
LR  - 20181202
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 109
DP  - 2016 Dec
TI  - Detection of porcine reproductive and respiratory syndrome virus (PRRSV) and 
      influenza A virus (IAV) in oral fluid of pigs.
PG  - 74-80
LID - S0034-5288(16)30365-4 [pii]
LID - 10.1016/j.rvsc.2016.09.014 [doi]
AB  - Recently oral fluid has become a novel sample type for pathogen nucleic acid and 
      antibody detection, as it is easy to obtain with non-invasive procedures. The 
      objective of the study was to analyze porcine reproductive and respiratory 
      syndrome virus (PRRSV) and influenza A virus (IAV) circulation in growing pigs 
      from three Polish production farms, using Real Time PCR and ELISA testing of oral 
      fluid and serum. Oral fluids were collected every 2weeks, in the same 3-4 pens of 
      pigs aged between 5 and 17weeks. Additionally, blood samples were collected every 
      4weeks from 4 pigs corresponding to the same pens as oral fluid and tested for 
      the presence of PRRSV nucleic acid (pooled by 4) and antibodies. In farm A no 
      PRRSV circulation was detected and only maternal antibodies were present. In farm 
      B and farm C antibodies to PRRSV in serum and oral fluid were detected in most 
      samples. In farm B PRRSV Type 1 was detected in 80.9% of oral fluid samples and 
      in 58.3% of serum pools, and in farm C in 92.8% of oral fluid samples and 75% 
      serum pools. Striking differences were observed between different pens in PRRSV 
      detection patterns. In farms B and C ORF5 sequence analysis showed the presence 
      of wild type strains which were about 84-85% identical to the modified live 
      vaccine used. In all three farms two waves of IAV shedding with oral fluid were 
      detected, in weaners and fatteners.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Biernacka, Kinga
AU  - Biernacka K
AD  - Warsaw University of Life Sciences - SGGW, Faculty of Veterinary Medicine, 
      Department of Pathology and Veterinary Diagnostics, Nowoursynowska 159c, 02-776 
      Warsaw, Poland.
FAU - Karbowiak, Pawel
AU  - Karbowiak P
AD  - Vet-Com L.L.C., Jagiellonska 71, 10-237 Olsztyn, Poland.
FAU - Wrobel, Pawel
AU  - Wrobel P
AD  - Swine Vet Consulting L.L.C., Bolewskiego 40, 63-700 Krotoszyn, Poland.
FAU - Chareza, Tomasz
AU  - Chareza T
AD  - Poldanor SA, Dworcowa 25, 77-320 Przechlewo, Poland.
FAU - Czopowicz, Michal
AU  - Czopowicz M
AD  - Warsaw University of Life Sciences - SGGW, Faculty of Veterinary Medicine, 
      Laboratory of Veterinary Epidemiology and Economics, Nowoursynowska 159c, 02-776 
      Warsaw, Poland.
FAU - Balka, Gyula
AU  - Balka G
AD  - University of Veterinary Medicine, Department of Pathology, Istvan u. 2, H-1078 
      Budapest, Hungary.
FAU - Goodell, Christa
AU  - Goodell C
AD  - IDEXX Laboratories, Inc., Westbrook, ME, USA.
FAU - Rauh, Rolf
AU  - Rauh R
AD  - Tetracore Inc, 9901 Belward Campus Drive Suite 300, Rockville, MD 20850, USA.
FAU - Stadejek, Tomasz
AU  - Stadejek T
AD  - Warsaw University of Life Sciences - SGGW, Faculty of Veterinary Medicine, 
      Department of Pathology and Veterinary Diagnostics, Nowoursynowska 159c, 02-776 
      Warsaw, Poland. Electronic address: tomasz_stadejek@sggw.pl.
LA  - eng
PT  - Journal Article
DEP - 20160921
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/analysis
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Epidemiological Monitoring/*veterinary
MH  - Female
MH  - Influenza A virus/*isolation & purification
MH  - Orthomyxoviridae Infections/diagnosis/*veterinary
MH  - Phylogeny
MH  - Poland/epidemiology
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - Real-Time Polymerase Chain Reaction/veterinary
MH  - Saliva/virology
MH  - Sensitivity and Specificity
MH  - Sequence Analysis, DNA/veterinary
MH  - Swine
MH  - Swine Diseases/*diagnosis
MH  - Virus Shedding
OTO - NOTNLM
OT  - ELISA
OT  - IAV
OT  - Oral fluid
OT  - PRRSV
OT  - Real time PCR
EDAT- 2016/11/29 06:00
MHDA- 2017/02/22 06:00
CRDT- 2016/11/29 06:00
PHST- 2016/04/25 00:00 [received]
PHST- 2016/09/15 00:00 [revised]
PHST- 2016/09/20 00:00 [accepted]
PHST- 2016/11/29 06:00 [pubmed]
PHST- 2017/02/22 06:00 [medline]
PHST- 2016/11/29 06:00 [entrez]
AID - S0034-5288(16)30365-4 [pii]
AID - 10.1016/j.rvsc.2016.09.014 [doi]
PST - ppublish
SO  - Res Vet Sci. 2016 Dec;109:74-80. doi: 10.1016/j.rvsc.2016.09.014. Epub 2016 Sep 
      21.