PMID- 27894314
OWN - NLM
STAT- MEDLINE
DCOM- 20170822
LR  - 20201209
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 13
IP  - 1
DP  - 2016 Nov 29
TI  - Self-assembly of hexahistidine-tagged tobacco etch virus capsid protein into 
      microfilaments that induce IgG2-specific response against a soluble porcine 
      reproductive and respiratory syndrome virus chimeric protein.
PG  - 196
LID - 196
AB  - BACKGROUND: Assembly of recombinant capsid proteins into virus-like particles 
      (VLPs) still represents an interesting challenge in virus-based nanotechnologies. 
      The structure of VLPs has gained importance for the development and design of new 
      adjuvants and antigen carriers. The potential of Tobacco etch virus capsid 
      protein (TEV CP) as adjuvant has not been evaluated to date. FINDINGS: Two 
      constructs for TEV CP expression in Escherichia coli were generated: a wild-type 
      version (TEV-CP) and a C-terminal hexahistidine (His)-tagged version 
      (His-TEV-CP). Although both versions were expressed in the soluble fraction of E. 
      coli lysates, only His-TEV-CP self-assembled into micrometric flexuous 
      filamentous VLPs. In addition, the His-tag enabled high yields and facilitated 
      purification of TEV VLPs. These TEV VLPs elicited broader IgG2-specific antibody 
      response against a novel porcine reproductive and respiratory syndrome virus 
      (PRRSV) protein when compared to the potent IgG1 response induced by the protein 
      alone. CONCLUSIONS: His-TEV CP was purified by immobilized metal affinity 
      chromatography and assembled into VLPs, some of them reaching 2-mum length. TEV 
      VLPs administered along with PRRSV chimeric protein changed the IgG2/IgG1 ratio 
      against the chimeric protein, suggesting that TEV CP can modulate the immune 
      response against a soluble antigen.
FAU - Manuel-Cabrera, Carlos Alberto
AU  - Manuel-Cabrera CA
AD  - Unidad de Biotecnologia Medica y Farmaceutica, Centro de Investigacion y 
      Asistencia en Tecnologia y Diseno del Estado de Jalisco A.C., Normalistas 800, 
      Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico.
FAU - Vallejo-Cardona, Alba Adriana
AU  - Vallejo-Cardona AA
AD  - Unidad de Biotecnologia Medica y Farmaceutica, Centro de Investigacion y 
      Asistencia en Tecnologia y Diseno del Estado de Jalisco A.C., Normalistas 800, 
      Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico.
FAU - Padilla-Camberos, Eduardo
AU  - Padilla-Camberos E
AD  - Unidad de Biotecnologia Medica y Farmaceutica, Centro de Investigacion y 
      Asistencia en Tecnologia y Diseno del Estado de Jalisco A.C., Normalistas 800, 
      Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico.
FAU - Hernandez-Gutierrez, Rodolfo
AU  - Hernandez-Gutierrez R
AD  - Unidad de Biotecnologia Medica y Farmaceutica, Centro de Investigacion y 
      Asistencia en Tecnologia y Diseno del Estado de Jalisco A.C., Normalistas 800, 
      Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico.
FAU - Herrera-Rodriguez, Sara Elisa
AU  - Herrera-Rodriguez SE
AD  - Unidad de Biotecnologia Medica y Farmaceutica, Centro de Investigacion y 
      Asistencia en Tecnologia y Diseno del Estado de Jalisco A.C., Normalistas 800, 
      Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico.
FAU - Gutierrez-Ortega, Abel
AU  - Gutierrez-Ortega A
AUID- ORCID: 0000-0002-5772-3950
AD  - Unidad de Biotecnologia Medica y Farmaceutica, Centro de Investigacion y 
      Asistencia en Tecnologia y Diseno del Estado de Jalisco A.C., Normalistas 800, 
      Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico. aortega@ciatej.mx.
LA  - eng
PT  - Journal Article
DEP - 20161129
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Capsid Proteins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (Virosomes)
SB  - IM
MH  - Actin Cytoskeleton/metabolism
MH  - Adjuvants, Immunologic/*administration & dosage/metabolism
MH  - Antibodies, Viral/*blood
MH  - Capsid Proteins/*administration & dosage/metabolism
MH  - Escherichia coli/genetics/metabolism
MH  - Gene Expression
MH  - Immunoglobulin G/blood
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Potyvirus/*immunology
MH  - Protein Multimerization
MH  - Recombinant Proteins/genetics/immunology/metabolism
MH  - Viral Vaccines/*immunology
MH  - Virosomes/*administration & dosage/metabolism
PMC - PMC5126848
OTO - NOTNLM
OT  - Adjuvant
OT  - Capsid protein
OT  - Chimeric protein
OT  - Hexahistidine tag
OT  - Tobacco etch virus
OT  - Virus-like particles
EDAT- 2016/11/30 06:00
MHDA- 2017/08/23 06:00
PMCR- 2016/11/29
CRDT- 2016/11/30 06:00
PHST- 2016/07/05 00:00 [received]
PHST- 2016/11/17 00:00 [accepted]
PHST- 2016/11/30 06:00 [entrez]
PHST- 2016/11/30 06:00 [pubmed]
PHST- 2017/08/23 06:00 [medline]
PHST- 2016/11/29 00:00 [pmc-release]
AID - 10.1186/s12985-016-0651-y [pii]
AID - 651 [pii]
AID - 10.1186/s12985-016-0651-y [doi]
PST - epublish
SO  - Virol J. 2016 Nov 29;13(1):196. doi: 10.1186/s12985-016-0651-y.