PMID- 27894405
OWN - NLM
STAT- MEDLINE
DCOM- 20170208
LR  - 20181023
IS  - 1165-158X (Electronic)
IS  - 0145-5680 (Linking)
VI  - 62
IP  - 12
DP  - 2016 Oct 31
TI  - Inhibition of matrix metalloproteins 9 attenuated Candida albicans induced 
      inflammation in mouse cornea.
PG  - 79-83
LID - 10.14715/cmb/2016.62.12.14 [doi]
AB  - Since the severe corneal ulceration of mouse cornea is known to occur with 
      inflammation. As one of imperative matrix metalloproteinase, the potential roles 
      of matrix metalloproteins 9 (MMP9) in corneal ulceration and keratitis are still 
      unveiled caused by fungal invasion. In this study, Candida albicans (CA) 
      inoculated wild-type KM mice cornea was used as a model pathogen in corneal 
      inflammation.  CA invasion significantly stimulated the expression of collagen IV 
      and MMP9 detected by RT-PCR, Real-time PCR and Immunofluorescent staining in 
      mouse cornea as soon as 6 hours post infection, and relatively decreased at 1 day 
      post infection. For examining the role of MMP9 in fungal keratitis, the mice 
      corneas were subconjunctivally injected MMP9 antibody or recombinant MMP9 protein 
      6 hours prior to CA inoculation, using rabbit IgG as control. Subconjunctival 
      injection of recombinant MMP9 protein prior to CA inoculation enhanced, whereas 
      MMP9 antibody attenuated corneal ulceration and inflammation, examining basement 
      membrane, fungal load, myeloperoxidase (MPO) and proinflammatory cytokines 
      including Macrophage inflammatory protein 2 (MIP2), Interleukin-1beta (IL-1beta) and 
      Tumor necrosis factor-alpha (TNF-alpha). Inhibition of MMP9 could potentially attenuate 
      Candida albicans induced inflammation in mouse cornea.
FAU - Dong, C
AU  - Dong C
AD  - College of Biological Engineering, Henan University of Technology, Zhengzhou, 
      Henan, China. chen.dong@haut.edu.cn.
FAU - Yang, M G
AU  - Yang MG
AD  - College of Biological Engineering, Henan University of Technology, Zhengzhou, 
      Henan, China.
LA  - eng
PT  - Journal Article
DEP - 20161031
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
RN  - 0 (Antibodies)
RN  - 0 (Chemokine CXCL2)
RN  - 0 (Collagen Type IV)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Animals
MH  - Antibodies/*pharmacology
MH  - Candida albicans/*enzymology
MH  - Chemokine CXCL2/analysis/genetics/metabolism
MH  - Collagen Type IV/metabolism
MH  - Cornea/*microbiology/pathology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Inflammation/*prevention & control
MH  - Interleukin-1beta/analysis/genetics/metabolism
MH  - Matrix Metalloproteinase 9/genetics/immunology/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microscopy, Fluorescence
MH  - Peroxidase/analysis/genetics/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Recombinant Proteins/biosynthesis/isolation & purification/pharmacology
MH  - Tumor Necrosis Factor-alpha/analysis/genetics/metabolism
EDAT- 2016/11/30 06:00
MHDA- 2017/02/09 06:00
CRDT- 2016/11/30 06:00
PHST- 2016/11/26 00:00 [received]
PHST- 2016/11/26 00:00 [accepted]
PHST- 2016/11/30 06:00 [entrez]
PHST- 2016/11/30 06:00 [pubmed]
PHST- 2017/02/09 06:00 [medline]
AID - 10.14715/cmb/2016.62.12.14 [doi]
PST - epublish
SO  - Cell Mol Biol (Noisy-le-grand). 2016 Oct 31;62(12):79-83. doi: 
      10.14715/cmb/2016.62.12.14.