PMID- 27894940
OWN - NLM
STAT- MEDLINE
DCOM- 20170512
LR  - 20181202
IS  - 1879-3185 (Electronic)
IS  - 0300-483X (Linking)
VI  - 375
DP  - 2017 Jan 15
TI  - Changes in the mRNA expression of structural proteins, hormone synthesis and 
      secretion from bovine placentome sections after DDT and DDE treatment.
PG  - 1-9
LID - S0300-483X(16)30295-5 [pii]
LID - 10.1016/j.tox.2016.11.016 [doi]
AB  - Disorders in the barrier function and secretory activity of the placenta can be 
      caused by xenobiotics (XB) present in the environment and their accumulation in 
      tissues of living organisms. Thus, the aim of this study was to investigate the 
      effect of 1,1,1-trichloro-2,2,-bis-4-chlorophenyl-ethane (DDT) and its metabolite 
      1,1-dichloro-2,2-bis-4-chlorophenyl-ethene (DDE) (for 24 or 48h) at doses of 1, 
      10 or 100ng/ml on the function of cow placentome sections in the second trimester 
      of pregnancy. DDT and DDE affected neither (P>0.05) the viability nor hypoxia 
      inducible factor 1 (HIF1alpha) mRNA expression of the sections. XB decreased (P<0.05) 
      connexin (Cx) 26, 32, 43 and placenta-specific 1 (PLAC-1) mRNA expression but did 
      not affect (P>0.05) keratin 8 (KRT8) mRNA expression. DDT and DDE also reduced 
      (P<0.05) prostaglandin F2alpha (PGF2alpha) synthase (PGFS) mRNA expression, while DDT 
      increased (P<0.05) prostaglandin E2 (PGE2) synthase (PGES) mRNA expression. 
      Neither cyclooxygenase 2 (COX-2) mRNA expression nor PGF2alpha and PGE2 secretion 
      were affected. Both DDT and DDE increased (P<0.05) neurophysin I/oxytocin 
      (NP1/OT) mRNA expression and oxytocin (OT), oestradiol (E2) and progesterone (P4) 
      secretion while DDT stimulated only 3beta-hydroxysteroid dehydrogenase (3betaHSD) and 
      cholesterol side-chain cleavage enzyme (CYP11A1) mRNA expression (P<0.05). In 
      summary, DDT and DDE impaired the barrier function and secretory activity of the 
      placenta. Thus, these compounds can disrupt trophoblast invasion, myometrium 
      contractility and gas/nutrient exchange throughout pregnancy in cows.
CI  - Copyright (c) 2016 Elsevier Ireland Ltd. All rights reserved.
FAU - Wojciechowska, A
AU  - Wojciechowska A
AD  - Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 
      Tuwima 10, 10-747 Olsztyn, Poland.
FAU - Mlynarczuk, J
AU  - Mlynarczuk J
AD  - Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 
      Tuwima 10, 10-747 Olsztyn, Poland. Electronic address: 
      j.mlynarczuk@pan.olsztyn.pl.
FAU - Kotwica, J
AU  - Kotwica J
AD  - Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 
      Tuwima 10, 10-747 Olsztyn, Poland.
LA  - eng
PT  - Journal Article
DEP - 20161125
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Connexins)
RN  - 0 (Insecticides)
RN  - 0 (PLAC1 protein, human)
RN  - 0 (Placental Hormones)
RN  - 0 (Pregnancy Proteins)
RN  - 0 (RNA, Messenger)
RN  - 4M7FS82U08 (Dichlorodiphenyl Dichloroethylene)
RN  - CIW5S16655 (DDT)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Connexins/biosynthesis/genetics
MH  - DDT/*toxicity
MH  - Dichlorodiphenyl Dichloroethylene/*toxicity
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - Insecticides/toxicity
MH  - Placenta/*drug effects/*metabolism
MH  - Placental Hormones/*biosynthesis/genetics/metabolism
MH  - Pregnancy
MH  - Pregnancy Proteins/biosynthesis/genetics
MH  - RNA, Messenger/*biosynthesis/genetics
OTO - NOTNLM
OT  - Connexins
OT  - DDE
OT  - DDT
OT  - Oxytocin
OT  - Placenta
OT  - Prostaglandins
EDAT- 2016/11/30 06:00
MHDA- 2017/05/13 06:00
CRDT- 2016/11/30 06:00
PHST- 2016/05/12 00:00 [received]
PHST- 2016/06/14 00:00 [revised]
PHST- 2016/11/25 00:00 [accepted]
PHST- 2016/11/30 06:00 [pubmed]
PHST- 2017/05/13 06:00 [medline]
PHST- 2016/11/30 06:00 [entrez]
AID - S0300-483X(16)30295-5 [pii]
AID - 10.1016/j.tox.2016.11.016 [doi]
PST - ppublish
SO  - Toxicology. 2017 Jan 15;375:1-9. doi: 10.1016/j.tox.2016.11.016. Epub 2016 Nov 
      25.