PMID- 27895621
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1664-2392 (Print)
IS  - 1664-2392 (Electronic)
IS  - 1664-2392 (Linking)
VI  - 7
DP  - 2016
TI  - Immune Modulatory Effects of Human Chorionic Gonadotropin on Dendritic Cells 
      Supporting Fetal Survival in Murine Pregnancy.
PG  - 146
LID - 146
AB  - Dendritic cells (DCs) are critically involved in the determination of immunity 
      vs. tolerance. Hence, DCs are key regulators of immune responses either favoring 
      or disfavoring fetal survival. Several factors were proposed to modulate DC 
      phenotype and function during pregnancy. Here, we studied whether the pregnancy 
      hormone human chorionic gonadotropin (hCG) is involved in DC regulation. In 
      vitro, bone marrow-derived DCs (BMDCs) were stimulated in the presence or absence 
      of urine-purified or recombinant hCG (rhCG) preparations. Subsequently, BMDC 
      maturation was assessed. Cytokine secretion of activated BMDCs and their 
      capability to enforce TH1, TH2, TH17, or Treg cell differentiation was determined 
      after rhCG treatment. Moreover, the in vivo potential of hCG-modulated BMDCs to 
      influence pregnancy outcome, Treg cell number, and local cytokine expression was 
      evaluated after adoptive transfer in a murine abortion-prone model before and 
      after conception. Both hCG preparations impaired the maturation process of BMDCs. 
      rhCG treatment did neither alter cytokine secretion by BMDCs nor their ability to 
      drive TH1, TH2, or TH17 differentiation. rhCG-treated BMDCs augmented the number 
      of Treg cells within the T cell population. Adoptive transfer of rhCG-treated 
      BMDCs after conception did not influence pregnancy outcome. However, transfer of 
      hCG-treated BMDCs prior to mating had a protective effect on pregnancy. This 
      positive effect was accompanied by increased Treg cell numbers and decidual IL-10 
      and TGF-beta expression. Our results unveil the importance of hCG in retaining DCs 
      in a tolerogenic state, thereby promoting Treg cell increment and supporting 
      fetal survival.
FAU - Dauven, Dominique
AU  - Dauven D
AD  - Department of Experimental Obstetrics and Gynecology, Medical Faculty, 
      Otto-von-Guericke University , Magdeburg , Germany.
FAU - Ehrentraut, Stefanie
AU  - Ehrentraut S
AD  - Department of Experimental Obstetrics and Gynecology, Medical Faculty, 
      Otto-von-Guericke University , Magdeburg , Germany.
FAU - Langwisch, Stefanie
AU  - Langwisch S
AD  - Department of Experimental Obstetrics and Gynecology, Medical Faculty, 
      Otto-von-Guericke University , Magdeburg , Germany.
FAU - Zenclussen, Ana Claudia
AU  - Zenclussen AC
AD  - Department of Experimental Obstetrics and Gynecology, Medical Faculty, 
      Otto-von-Guericke University , Magdeburg , Germany.
FAU - Schumacher, Anne
AU  - Schumacher A
AD  - Department of Experimental Obstetrics and Gynecology, Medical Faculty, 
      Otto-von-Guericke University , Magdeburg , Germany.
LA  - eng
PT  - Journal Article
DEP - 20161115
PL  - Switzerland
TA  - Front Endocrinol (Lausanne)
JT  - Frontiers in endocrinology
JID - 101555782
PMC - PMC5108759
OTO - NOTNLM
OT  - dendritic cells
OT  - fetal tolerance
OT  - human chorionic gonadotropin
OT  - pregnancy
OT  - regulatory T cells
EDAT- 2016/11/30 06:00
MHDA- 2016/11/30 06:01
PMCR- 2016/01/01
CRDT- 2016/11/30 06:00
PHST- 2016/09/09 00:00 [received]
PHST- 2016/11/01 00:00 [accepted]
PHST- 2016/11/30 06:00 [entrez]
PHST- 2016/11/30 06:00 [pubmed]
PHST- 2016/11/30 06:01 [medline]
PHST- 2016/01/01 00:00 [pmc-release]
AID - 10.3389/fendo.2016.00146 [doi]
PST - epublish
SO  - Front Endocrinol (Lausanne). 2016 Nov 15;7:146. doi: 10.3389/fendo.2016.00146. 
      eCollection 2016.