PMID- 27898057
OWN - NLM
STAT- MEDLINE
DCOM- 20180524
LR  - 20220321
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Nov 29
TI  - Functional brain mapping using specific sensory-circuit stimulation and a 
      theoretical graph network analysis in mice with neuropathic allodynia.
PG  - 37802
LID - 10.1038/srep37802 [doi]
LID - 37802
AB  - Allodynia, a form of neuropathic pain, is defined as pain in response to a 
      non-nociceptive stimulus. The brain regions responsible for pain, which are not 
      normally activated, can be activated in allodynic mice by providing a suitable 
      stimulus to Abeta-fibers, which transmit signals from tactile sensory fibers. 
      Functional MRI (fMRI) can be used to objectively observe abnormal brain 
      activation. In the present study, fMRI was conducted to investigate allodynia in 
      mice; allodynia was generated by surgical injury at the L4 spinal nerve root, 
      thus selectively stimulating sensory nerve fibers. In intact mice, only the 
      primary somatosensory cortex (S1) was activated by stimulation of Abeta-fibers. 
      Meanwhile, allodynic mice showed significantly higher BOLD signals in the 
      anterior cingulate area (ACA) and thalamus. Using resting state fMRI, both degree 
      and eigenvector centrality were significantly decreased in the contralateral S1, 
      clustering coefficient and local efficiency were significantly increased in the 
      ACA, and betweenness centrality was significantly higher in the ventral 
      posterolateral nucleus of the thalamus. These results suggest that the observed 
      abnormal BOLD activation is associated with defects in Abeta-fibers when Abeta-fibers 
      in allodynic mice are selectively stimulated. The objective approach enabled by 
      fMRI can improve our understanding of pathophysiological mechanisms and 
      therapeutic efficacy.
FAU - Komaki, Yuji
AU  - Komaki Y
AD  - Department of Physiology, Keio University School of Medicine, Shinjuku-ku, Tokyo 
      160-8582, Japan.
AD  - Imaging Analysis Laboratory, Laboratory Animal Research Department, Central 
      Institute for Experimental Animals, Kawasaki-shi, Kanagawa 210-0821, Japan.
FAU - Hikishima, Keigo
AU  - Hikishima K
AD  - Department of Physiology, Keio University School of Medicine, Shinjuku-ku, Tokyo 
      160-8582, Japan.
AD  - Imaging Analysis Laboratory, Laboratory Animal Research Department, Central 
      Institute for Experimental Animals, Kawasaki-shi, Kanagawa 210-0821, Japan.
FAU - Shibata, Shinsuke
AU  - Shibata S
AD  - Department of Physiology, Keio University School of Medicine, Shinjuku-ku, Tokyo 
      160-8582, Japan.
FAU - Konomi, Tsunehiko
AU  - Konomi T
AD  - Department of Orthopaedic Surgery, Keio University School of Medicine, 
      Shinjuku-ku, Tokyo 160-8582, Japan.
AD  - Department of Orthopedic Surgery, National Hospital Organization Murayama Medical 
      Center, Musashimurayama-shi, Tokyo 208-0011, Japan.
FAU - Seki, Fumiko
AU  - Seki F
AD  - Department of Physiology, Keio University School of Medicine, Shinjuku-ku, Tokyo 
      160-8582, Japan.
AD  - Imaging Analysis Laboratory, Laboratory Animal Research Department, Central 
      Institute for Experimental Animals, Kawasaki-shi, Kanagawa 210-0821, Japan.
FAU - Yamada, Masayuki
AU  - Yamada M
AD  - Faculty of Radiological Technology, School of Health Sciences, Fujita Health 
      University, Toyoake-shi, Aichi 470-1192, Japan.
FAU - Miyasaka, Naoyuki
AU  - Miyasaka N
AD  - Department of Pediatrics, Perinatal and Maternal Medicine, Graduate School, Tokyo 
      Medical and Dental University, Bunkyo-ku Tokyo 113-8519, Japan.
FAU - Fujiyoshi, Kanehiro
AU  - Fujiyoshi K
AD  - Department of Orthopaedic Surgery, Keio University School of Medicine, 
      Shinjuku-ku, Tokyo 160-8582, Japan.
AD  - Department of Orthopedic Surgery, National Hospital Organization Murayama Medical 
      Center, Musashimurayama-shi, Tokyo 208-0011, Japan.
FAU - Okano, Hirotaka J
AU  - Okano HJ
AD  - Division of Regenerative Medicine, The Jikei University School of Medicine, 
      Minato-ku, Tokyo 105-8461, Japan.
FAU - Nakamura, Masaya
AU  - Nakamura M
AD  - Department of Orthopaedic Surgery, Keio University School of Medicine, 
      Shinjuku-ku, Tokyo 160-8582, Japan.
FAU - Okano, Hideyuki
AU  - Okano H
AD  - Department of Physiology, Keio University School of Medicine, Shinjuku-ku, Tokyo 
      160-8582, Japan.
AD  - Laboratory for Marmoset Neural Architecture, Brain Science Institute RIKEN, 2-1 
      Hirosawa, Wako, Saitama 351-0198, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161129
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - Animals
MH  - Brain/diagnostic imaging/*physiology
MH  - Disease Models, Animal
MH  - Functional Neuroimaging/*methods
MH  - Hyperalgesia/*diagnosis
MH  - Magnetic Resonance Imaging
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nerve Fibers/*physiology
MH  - Pain
MH  - Physical Stimulation
MH  - Sensory Receptor Cells/*physiology
MH  - Spinal Nerves/surgery
MH  - Touch
PMC - PMC5127182
COIS- H.O. is a paid scientific consultant for San Bio, Co., Ltd. All other authors 
      declare that they have no competing financial interests.
EDAT- 2016/11/30 06:00
MHDA- 2018/05/25 06:00
PMCR- 2016/11/29
CRDT- 2016/11/30 06:00
PHST- 2016/05/25 00:00 [received]
PHST- 2016/11/02 00:00 [accepted]
PHST- 2016/11/30 06:00 [entrez]
PHST- 2016/11/30 06:00 [pubmed]
PHST- 2018/05/25 06:00 [medline]
PHST- 2016/11/29 00:00 [pmc-release]
AID - srep37802 [pii]
AID - 10.1038/srep37802 [doi]
PST - epublish
SO  - Sci Rep. 2016 Nov 29;6:37802. doi: 10.1038/srep37802.