PMID- 27898957
OWN - NLM
STAT- MEDLINE
DCOM- 20170420
LR  - 20181202
IS  - 1525-3163 (Electronic)
IS  - 0021-8812 (Linking)
VI  - 94
IP  - 11
DP  - 2016 Nov
TI  - Supplementation of corn dried distillers' grains plus solubles to gestating beef 
      cows fed low-quality forage: II. Impacts on uterine blood flow, circulating 
      estradiol-17beta and progesterone, and hepatic steroid metabolizing enzyme activity.
PG  - 4619-4628
LID - 10.2527/jas.2016-0400 [doi]
AB  - The objective of this study was to investigate the effects of supplementing dried 
      distillers' grains plus solubles (DDGS) during late gestation on uterine blood 
      flow (BF), circulating steroid hormones and hepatic steroid metabolizing enzymes, 
      and calf and placental weights. Multiparous beef cows were randomly divided into 
      a control group (CON; = 15) consuming a diet containing 90% corn stover and 10% 
      corn silage (DM basis) for ad libitum intake and a treatment group (SUP; = 12) 
      consuming the same diet and DDGS (0.3% of BW). Corn silage inclusion was 
      increased to 30% as gestation progressed to meet increasing caloric requirements. 
      Ipsilateral and contralateral uterine BF and cross-sectional area (CSA) of each 
      uterine artery were measured by Doppler ultrasonography on d 180, 216, and 246 of 
      pregnancy. Contralateral BF and CSA increased ( < 0.01) as gestation advanced. 
      Ipsilateral BF and CSA was affected by a treatment x day of gestation interaction 
      ( < 0.05). A main effect of treatment ( = 0.02) and day ( < 0.01) was observed 
      for total BF; BF increased over time and SUP cows had greater BF than CON cows. 
      Circulating concentrations of both progesterone (P4) and estradiol-17beta (E2) were 
      affected by an interaction of treatment and day ( < 0.01). Concentrations of 
      circulating E2 steadily increased throughout the study and were greater in CON 
      cows than in SUP cows by d 242. Concentrations of P4 also increased over time; P4 
      of CON cows was greater than that of SUP cows by d 242. Uridine 
      5'-diphospho-glucuronosyltransferase (UGT) and cytochrome P450 1A (CYP1A) 
      activity increased with advancing gestation ( < 0.01). There was greater UGT 
      activity ( < 0.05) and a trend for greater CYP1A activity ( = 0.06) in SUP cows 
      than in CON cows. Activity of cytochrome P450 3A was greater ( < 0.01) in SUP 
      cows and decreased ( < 0.05) with advancing gestation. Supplementing DDGS to cows 
      fed low-quality forage during late gestation increased uterine BF but decreased 
      circulating E2 and P4 concentrations and altered hepatic steroid metabolizing 
      enzyme activity. It was anticipated that enzyme activity would reflect 
      circulating hormone levels; however, our data suggests the observed increases in 
      BF are not driven by alterations in hormone concentration. Therefore, further 
      research is warranted to elucidate the underlying mechanisms.
FAU - Kennedy, V C
AU  - Kennedy VC
FAU - Mordhorst, B R
AU  - Mordhorst BR
FAU - Gaspers, J J
AU  - Gaspers JJ
FAU - Bauer, M L
AU  - Bauer ML
FAU - Swanson, K C
AU  - Swanson KC
FAU - Lemley, C O
AU  - Lemley CO
FAU - Vonnahme, K A
AU  - Vonnahme KA
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PL  - United States
TA  - J Anim Sci
JT  - Journal of animal science
JID - 8003002
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
SB  - IM
MH  - Animal Feed/*analysis
MH  - Animal Nutritional Physiological Phenomena
MH  - Animals
MH  - Cattle/*physiology
MH  - Cytochrome P-450 Enzyme System/genetics/metabolism
MH  - Diet/*veterinary
MH  - Dietary Supplements
MH  - Estradiol/*blood
MH  - Feeding Behavior
MH  - Female
MH  - Gene Expression Regulation, Enzymologic/drug effects
MH  - Lipid Metabolism
MH  - Liver/enzymology
MH  - Nutritive Value
MH  - Pregnancy
MH  - Progesterone/*blood
MH  - *Zea mays
EDAT- 2016/11/30 06:00
MHDA- 2017/04/21 06:00
CRDT- 2016/11/30 06:00
PHST- 2016/11/30 06:00 [entrez]
PHST- 2016/11/30 06:00 [pubmed]
PHST- 2017/04/21 06:00 [medline]
AID - 10.2527/jas.2016-0400 [doi]
PST - ppublish
SO  - J Anim Sci. 2016 Nov;94(11):4619-4628. doi: 10.2527/jas.2016-0400.