PMID- 2790039
OWN - NLM
STAT- MEDLINE
DCOM- 19891107
LR  - 20190609
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1013
IP  - 1
DP  - 1989 Sep 4
TI  - Phorbol esters and chemotactic factor induce distinct changes in cytoplasmic Ca2+ 
      and pH in granulocytic like HL60 cells.
PG  - 55-9
AB  - Differentiation of HL60 cells into neutrophil-like cells after exposure to 
      dimethylsulfoxide is accompanied by an increase in intracellular pH (pHi) which 
      results from an increased activity of the Na+/H+ antiporter at physiological pHi 
      value, but not at acidic pHi values. The functional responses of differentiated 
      HL60 cells to the chemotactic peptide, N-formylmethionylleucylphenylalanine 
      (fMLP), and to phorbol myristate acetate (PMA) were studied. In differentiated 
      cells fMLP produced a large increase in cytosolic Ca2+ levels and a small 
      biphasic change in pHi, whereas PMA produced cellular acidification, which was 
      potentiated by ethylisopropylamiloride and no change in [Ca2+]i. In 
      undifferentiated HL60 cells, PMA produced the opposite effect on pHi, i.e., a 
      cellular alkalinization. The extent of the acidification produced by PMA in 
      differentiated HL60 cells correlated with the production of reactive oxygen 
      species.
FAU - Ladoux, A
AU  - Ladoux A
AD  - Inserm U 204, Hopital Saint Louis, Paris, France.
FAU - Krawice, I
AU  - Krawice I
FAU - Damais, C
AU  - Damais C
FAU - Frelin, C
AU  - Frelin C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 7DZO8EB0Z3 (Amiloride)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SY7Q814VUP (Calcium)
RN  - VW50CE070T (ethylisopropylamiloride)
RN  - YOW8V9698H (Dimethyl Sulfoxide)
SB  - IM
MH  - Amiloride/analogs & derivatives/pharmacology
MH  - Calcium/*metabolism
MH  - Cell Differentiation/*drug effects
MH  - Cell Line
MH  - Dimethyl Sulfoxide/pharmacology
MH  - Granulocytes/drug effects/*metabolism
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Kinetics
MH  - Leukemia, Promyelocytic, Acute
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Tetradecanoylphorbol Acetate/*pharmacology
MH  - Tumor Cells, Cultured/drug effects/*metabolism
EDAT- 1989/09/04 00:00
MHDA- 1989/09/04 00:01
CRDT- 1989/09/04 00:00
PHST- 1989/09/04 00:00 [pubmed]
PHST- 1989/09/04 00:01 [medline]
PHST- 1989/09/04 00:00 [entrez]
AID - 0167-4889(89)90127-4 [pii]
AID - 10.1016/0167-4889(89)90127-4 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1989 Sep 4;1013(1):55-9. doi: 10.1016/0167-4889(89)90127-4.