PMID- 27900536
OWN - NLM
STAT- MEDLINE
DCOM- 20180102
LR  - 20181113
IS  - 1573-9368 (Electronic)
IS  - 0962-8819 (Linking)
VI  - 26
IP  - 1
DP  - 2017 Feb
TI  - Tbeta4-overexpression based on the piggyBac transposon system in cashmere goats 
      alters hair fiber characteristics.
PG  - 77-85
LID - 10.1007/s11248-016-9988-7 [doi]
AB  - Increasing cashmere yield is one of the vital aims of cashmere goats breeding. 
      Compared to traditional breeding methods, transgenic technology is more efficient 
      and the piggyBac (PB) transposon system has been widely applied to generate 
      transgenic animals. For the present study, donor fibroblasts were stably 
      transfected via a PB donor vector containing the coding sequence of cashmere goat 
      thymosin beta-4 (Tbeta4) and driven by a hair follicle-specific promoter, the 
      keratin-associated protein 6.1 (KAP6.1) promoter. To obtain genetically modified 
      cells as nuclear donors, we co-transfected donor vectors into fetal fibroblasts 
      of cashmere goats. Five transgenic cashmere goats were generated following 
      somatic cell nuclear transfer (SCNT). Via determination of the copy numbers and 
      integration sites, the Tbeta4 gene was successfully inserted into the goat genome. 
      Histological examination of skin tissue revealed that Tbeta4-overexpressing, 
      transgenic goats had a higher secondary to primary hair follicle (S/P) ratio 
      compared to wild type goats. This indicates that Tbeta4-overexpressing goats possess 
      increased numbers of secondary hair follicles (SHF). Our results indicate that 
      Tbeta4-overexpression in cashmere goats could be a feasible strategy to increase 
      cashmere yield.
FAU - Shi, Bingbo
AU  - Shi B
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      712100, China.
FAU - Ding, Qiang
AU  - Ding Q
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      712100, China.
FAU - He, Xiaolin
AU  - He X
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      712100, China.
FAU - Zhu, Haijing
AU  - Zhu H
AD  - Shaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, 
      Yulin, 719000, China.
AD  - Life Science Research Center, Yulin University, Yulin, 719000, China.
FAU - Niu, Yiyuan
AU  - Niu Y
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      712100, China.
FAU - Cai, Bei
AU  - Cai B
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      712100, China.
FAU - Cai, Jiao
AU  - Cai J
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, 
      China.
FAU - Lei, Anming
AU  - Lei A
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, 
      China.
FAU - Kang, Danju
AU  - Kang D
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      712100, China.
FAU - Yan, Hailong
AU  - Yan H
AD  - Shaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, 
      Yulin, 719000, China.
AD  - Life Science Research Center, Yulin University, Yulin, 719000, China.
FAU - Ma, Baohua
AU  - Ma B
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, 
      China.
FAU - Wang, Xiaolong
AU  - Wang X
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      712100, China.
FAU - Qu, Lei
AU  - Qu L
AD  - Shaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, 
      Yulin, 719000, China. ylqulei@126.com.
AD  - Life Science Research Center, Yulin University, Yulin, 719000, China. 
      ylqulei@126.com.
FAU - Chen, Yulin
AU  - Chen Y
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      712100, China. chenyulin@nwafu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161129
PL  - Netherlands
TA  - Transgenic Res
JT  - Transgenic research
JID - 9209120
RN  - 0 (DNA Transposable Elements)
RN  - 549LM7U24W (thymosin beta(4))
RN  - 61512-21-8 (Thymosin)
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified/*genetics/growth & development
MH  - DNA Transposable Elements/genetics
MH  - Fibroblasts/metabolism
MH  - Gene Expression Regulation
MH  - Goats/*genetics/growth & development
MH  - Hair Follicle/growth & development/metabolism
MH  - Keratins/*genetics/metabolism
MH  - Nuclear Transfer Techniques
MH  - Skin/growth & development/metabolism
MH  - Thymosin/*genetics/metabolism
OTO - NOTNLM
OT  - Cashmere goat
OT  - Hair follicle
OT  - SCNT
OT  - Tbeta4-overexpression
OT  - piggyBac transposon
EDAT- 2016/12/03 06:00
MHDA- 2018/01/03 06:00
CRDT- 2016/12/01 06:00
PHST- 2016/04/20 00:00 [received]
PHST- 2016/09/29 00:00 [accepted]
PHST- 2016/12/03 06:00 [pubmed]
PHST- 2018/01/03 06:00 [medline]
PHST- 2016/12/01 06:00 [entrez]
AID - 10.1007/s11248-016-9988-7 [pii]
AID - 10.1007/s11248-016-9988-7 [doi]
PST - ppublish
SO  - Transgenic Res. 2017 Feb;26(1):77-85. doi: 10.1007/s11248-016-9988-7. Epub 2016 
      Nov 29.