PMID- 27901381
OWN - NLM
STAT- MEDLINE
DCOM- 20170502
LR  - 20211204
IS  - 1205-7541 (Electronic)
IS  - 0008-4212 (Linking)
VI  - 95
IP  - 1
DP  - 2017 Jan
TI  - Effects of fisetin on hyperhomocysteinemia-induced experimental endothelial 
      dysfunction and vascular dementia.
PG  - 32-42
LID - 10.1139/cjpp-2016-0147 [doi]
AB  - This study was designed to investigate the effects of fisetin (FST) on 
      hyperhomocysteinemia (HHcy)-induced experimental endothelial dysfunction (ED) and 
      vascular dementia (VaD) in rats. Wistar rats were randomly divided into 8 groups: 
      control, vehicle control, l-methionine, FST (5, 10, and 25 mg/kg, p.o.), FST-per 
      se (25 mg/kg, p.o.), and donepezil (0.1 mg/kg, p.o.). l-Methionine administration 
      (1.7 g/kg, p.o.) for 32 days induced HHcy. ED and VaD induced by HHcy were 
      determined by vascular reactivity measurements, behavioral analysis using Morris 
      water maze and Y-maze, along with a biochemical and histological evaluation of 
      thoracic aorta and brain tissues. Administration of l-methionine developed 
      behavioral deficits; triggered brain lipid peroxidation (LPO); compromised brain 
      acetylcholinesterase activity (AChE); and reduced the levels of brain superoxide 
      dismutase (SOD), brain catalase (CAT), brain reduced glutathione (GSH), and serum 
      nitrite; and increased serum homocysteine and cholesterol levels. These effects 
      were accompanied by decreased vascular NO bioavailability, marked intimal 
      thickening of the aorta, and multiple necrotic foci in brain cortex. HHcy-induced 
      alterations in the activities of SOD, CAT, GSH, AChE, LPO, behavioral deficits, 
      ED, and histological aberrations were significantly attenuated by treatment with 
      fisetin in a dose-dependent manner. Collectively, our results indicate that 
      fisetin exerts endothelial and neuroprotective effects against HHcy-induced ED 
      and VaD.
FAU - Hemanth Kumar, Boyina
AU  - Hemanth Kumar B
AD  - a Department of Pharmacology, Anurag Group of Institutions (Formerly Lalitha 
      College of Pharmacy), Hyderabad, Telangana, India.
AD  - b Center for Pharmaceutical Sciences and Research and Development Cell, 
      Jawaharlal Nehru Technological University, Hyderabad, Telangana, India.
FAU - Arun Reddy, Ravula
AU  - Arun Reddy R
AD  - a Department of Pharmacology, Anurag Group of Institutions (Formerly Lalitha 
      College of Pharmacy), Hyderabad, Telangana, India.
AD  - c Graduate School of Biomedical Sciences, Rowan University, Stratford, NJ 08084, 
      USA.
FAU - Mahesh Kumar, Jerald
AU  - Mahesh Kumar J
AD  - d Animal House Facility, CSIR-Centre for Cellular and Molecular Biology (CCMB), 
      Hyderabad, Telangana 500007, India.
FAU - Dinesh Kumar, B
AU  - Dinesh Kumar B
AD  - e National Institute of Nutrition (ICMR), Food & Drug Toxicology Research Centre 
      (FDTRC), Hyderabad, Telangana 500007, India.
FAU - Diwan, Prakash V
AU  - Diwan PV
AD  - a Department of Pharmacology, Anurag Group of Institutions (Formerly Lalitha 
      College of Pharmacy), Hyderabad, Telangana, India.
AD  - f Maratha Mandal Central Research Laboratory, Belagavi-590010, Karnataka, India.
LA  - eng
PT  - Journal Article
DEP - 20160811
PL  - Canada
TA  - Can J Physiol Pharmacol
JT  - Canadian journal of physiology and pharmacology
JID - 0372712
RN  - 0 (Flavonoids)
RN  - 0 (Flavonols)
RN  - 0 (Indans)
RN  - 0 (Nitrites)
RN  - 0 (Piperidines)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 8SSC91326P (Donepezil)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - AE28F7PNPL (Methionine)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 3.1.1.7 (Acetylcholinesterase)
RN  - GAN16C9B8O (Glutathione)
RN  - OO2ABO9578 (fisetin)
SB  - IM
MH  - Acetylcholinesterase/metabolism
MH  - Animals
MH  - Aorta/pathology
MH  - Brain/metabolism
MH  - Catalase/metabolism
MH  - Cholesterol/blood
MH  - Dementia, Vascular/blood/complications/*drug therapy/metabolism
MH  - Donepezil
MH  - Dose-Response Relationship, Drug
MH  - Endothelium, Vascular/*drug effects
MH  - Flavonoids/*pharmacology/*therapeutic use
MH  - Flavonols
MH  - Glutathione/metabolism
MH  - Homocysteine/blood
MH  - Hyperhomocysteinemia/chemically induced/complications/*drug therapy/metabolism
MH  - Indans/therapeutic use
MH  - Lipid Peroxidation/drug effects
MH  - Male
MH  - Maze Learning
MH  - Methionine/adverse effects
MH  - Necrosis/drug therapy/pathology
MH  - Nitric Oxide/metabolism
MH  - Nitrites/blood
MH  - Piperidines/therapeutic use
MH  - Rats
MH  - Superoxide Dismutase/metabolism
OTO - NOTNLM
OT  - Morris water maze
OT  - acetylcholinesterase
OT  - acetylcholinesterase
OT  - foyers necrotiques
OT  - l-NAME
OT  - l-methionine
OT  - l-methionine
OT  - labyrinthe aquatique de Morris
OT  - necrotic foci
EDAT- 2016/12/03 06:00
MHDA- 2017/05/04 06:00
CRDT- 2016/12/01 06:00
PHST- 2016/12/03 06:00 [pubmed]
PHST- 2017/05/04 06:00 [medline]
PHST- 2016/12/01 06:00 [entrez]
AID - 10.1139/cjpp-2016-0147 [doi]
PST - ppublish
SO  - Can J Physiol Pharmacol. 2017 Jan;95(1):32-42. doi: 10.1139/cjpp-2016-0147. Epub 
      2016 Aug 11.