PMID- 27901503
OWN - NLM
STAT- MEDLINE
DCOM- 20170622
LR  - 20170622
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 122
IP  - 1
DP  - 2016 Nov 22
TI  - In situ hybridization and histopathological observations during ostreid 
      herpesvirus-1-associated mortalities in Pacific oysters Crassostrea gigas.
PG  - 43-55
AB  - In a previous longitudinal study conducted during a mortality investigation 
      associated with ostreid herpesvirus-1 (OsHV-1) microvariant in New Zealand 
      Pacific oysters in 2010-2011, temporality of OsHV-1 nucleic acid detection by 
      real-time PCR assay and onset of Pacific oyster mortality was observed. The 
      present study further elucidated the role of OsHV-1 using an in situ 
      hybridization (ISH) assay on sections of Pacific oysters collected from the same 
      longitudinal study. Hybridization of the labelled probe with the target region of 
      the OsHV-1 genome in infected cells was detected colorimetrically using nitro 
      blue tetrazolium (NBT). OsHV-1 presence and distribution in spat indicated by the 
      ISH signal was then compared with the existence of pathological changes in oyster 
      tissues. Dark blue to purplish black NBT cell labelling was seen predominantly in 
      the stroma of the mantle and gills at Day 5 post introduction to the farm. The 
      distribution and location of ISH signals indicated the extent of OsHV-1-infected 
      cells in multiple tissues. Histopathological abnormalities were mostly 
      non-specific; however, a progressive pattern of increasingly widespread 
      haemocytosis coincided with the appearance of OsHV-1-infected cells in spat 
      collected at different time-points. The visualisation of an increasing number of 
      OsHV-1-positive cells in spat prior to a marked increase in mortality indicated 
      the strong likelihood of an on-going and active viral infection in some oysters. 
      Further studies are recommended to elucidate OsHV-1 pathogenesis in Pacific 
      oysters in association with other potentially causal variables, such as elevated 
      temperature and interaction with Vibrio spp. bacteria.
FAU - Bueno, Rudolfo
AU  - Bueno R
AD  - Animal Health Laboratory, Investigation, Diagnostic Centres and 
      Response-Wallaceville, Ministry for Primary Industries, 66 Ward St, PO Box 40742, 
      Upper Hutt 5018, New Zealand.
FAU - Perrott, Matthew
AU  - Perrott M
FAU - Dunowska, Magda
AU  - Dunowska M
FAU - Brosnahan, Cara
AU  - Brosnahan C
FAU - Johnston, Colin
AU  - Johnston C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Crassostrea/*virology
MH  - DNA, Viral/genetics
MH  - Herpesviridae/isolation & purification/*physiology
MH  - Host-Pathogen Interactions
MH  - In Situ Hybridization
MH  - Real-Time Polymerase Chain Reaction
OTO - NOTNLM
OT  - Crassostrea gigas
OT  - In situ hybridization
OT  - Microvariant
OT  - Ostreid herpesvirus-1
OT  - Pacific oysters
OT  - muVar
EDAT- 2016/12/03 06:00
MHDA- 2017/06/24 06:00
CRDT- 2016/12/01 06:00
PHST- 2016/12/01 06:00 [entrez]
PHST- 2016/12/03 06:00 [pubmed]
PHST- 2017/06/24 06:00 [medline]
AID - 10.3354/dao03062 [doi]
PST - ppublish
SO  - Dis Aquat Organ. 2016 Nov 22;122(1):43-55. doi: 10.3354/dao03062.