PMID- 27902903
OWN - NLM
STAT- MEDLINE
DCOM- 20170728
LR  - 20220408
IS  - 1938-4351 (Electronic)
IS  - 0005-2086 (Linking)
VI  - 60
IP  - 4
DP  - 2016 Dec
TI  - Characterization of Thai Mycoplasma synoviae Isolates by Sequence Analysis of 
      Partial vlhA Gene.
PG  - 810-816
AB  - Mycoplasma synoviae (MS), a remarkable pathogen in poultry, causes subclinical 
      infection of the upper respiratory tract and an infectious synovitis, especially 
      in the tendon sheaths and synovial membranes of joints. Because the specific 
      detection of MS 16S rRNA gene-based PCR was unsuitable for strain 
      differentiation, vlhA gene-based PCR was designed to differentiate the MS 
      strains. The vlhA gene of MS encodes for hemagglutinin and other immunodominant 
      membrane proteins involved in colonization, antigenic variations, and virulence. 
      Sequence analysis of the vlhA gene based on the nucleotide insertion/deletion of 
      the proline-rich repeat (PRR) region and the nucleotide polymorphisms of the RIII 
      region in vlhA gene fragments was useful for typing and subtyping of MS strains. 
      This study aimed to characterize the Thai MS field isolates and to differentiate 
      the field and vaccine strains in Thailand by using sequence analysis of the 
      partial vlhA gene. In total, 20 MS field isolates submitted from registered 
      chicken farms in Thailand during 2015 were identified as Type C1 (n = 1), C2 (n = 
      4), E1 (n = 9), E2 (n = 1), and L (n = 5). The results revealed that six of the 
      nine isolates resulting in respiratory signs were Type E1. In addition, four 
      isolates from lame chickens showing joint swelling were identified as Type L, 
      with a length of 105 nucleotides. This study provides the first molecular data of 
      Thai MS isolates and the first evidence of Type L for being an arthropathic 
      strain that differs from a previous study demonstrating that only MS Type B, with 
      a longer PRR of 135 nucleotides, could be highly invasive strains and associated 
      with infectious synovitis in chickens. Furthermore, one farm showed coinfection 
      of MS Types E and L, but most of the farms were affected by only one type of MS. 
      The results indicated that sequence analysis of the partial vlhA gene can be used 
      as a tool for tracing MS characterization.
FAU - Limpavithayakul, Kriengwich
AU  - Limpavithayakul K
AD  - Avian Health Research Unit, Department of Veterinary Medicine, Faculty of 
      Veterinary Science, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand.
FAU - Sasipreeyajan, Jiroj
AU  - Sasipreeyajan J
AD  - Avian Health Research Unit, Department of Veterinary Medicine, Faculty of 
      Veterinary Science, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand.
FAU - Pakpinyo, Somsak
AU  - Pakpinyo S
AD  - Avian Health Research Unit, Department of Veterinary Medicine, Faculty of 
      Veterinary Science, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Avian Dis
JT  - Avian diseases
JID - 0370617
RN  - 0 (Bacterial Proteins)
RN  - 0 (Lectins)
RN  - 0 (VlhA protein, Mycoplasma synoviae)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Bacterial Proteins/chemistry/*genetics/metabolism
MH  - Chickens
MH  - Lectins/chemistry/*genetics/metabolism
MH  - Mycoplasma Infections/microbiology/*veterinary
MH  - Mycoplasma synoviae/chemistry/*genetics/metabolism
MH  - Poultry Diseases/*microbiology
MH  - Sequence Alignment
MH  - Thailand
OTO - NOTNLM
OT  - Mycoplasma synoviae isolates
OT  - PCR
OT  - chickens
OT  - typing
OT  - vlhA gene
EDAT- 2016/12/03 06:00
MHDA- 2017/07/29 06:00
CRDT- 2016/12/01 06:00
PHST- 2016/12/01 06:00 [entrez]
PHST- 2016/12/03 06:00 [pubmed]
PHST- 2017/07/29 06:00 [medline]
AID - 10.1637/11450-061216-Reg [doi]
PST - ppublish
SO  - Avian Dis. 2016 Dec;60(4):810-816. doi: 10.1637/11450-061216-Reg.